A5072006481- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Glycosylation and Glycoproteins Research
- RNA and protein synthesis mechanisms
- Vitamin C and Antioxidants Research
- Trace Elements in Health
- Monoclonal and Polyclonal Antibodies Research
- Cancer Research and Treatments
- Microbial Metabolism and Applications
- Salmonella and Campylobacter epidemiology
- Atherosclerosis and Cardiovascular Diseases
- Viral Infections and Outbreaks Research
- Bioactive Compounds and Antitumor Agents
- Mycobacterium research and diagnosis
- Cassava research and cyanide
- Plant nutrient uptake and metabolism
- Legume Nitrogen Fixing Symbiosis
- Folate and B Vitamins Research
- Protein Structure and Dynamics
- Computational Drug Discovery Methods
- Tuberculosis Research and Epidemiology
- Plant responses to water stress
- Enzyme Production and Characterization
- Morinda citrifolia extract uses
International Centre for Genetic Engineering and Biotechnology
2011-2024
GTx (United States)
2023
University of Arkansas for Medical Sciences
2017
International Centre for Genetic Engineering and Biotechnology
2009-2014
All India Institute of Medical Sciences
2014
Texas A&M University
2003-2011
Madurai Kamaraj University
1995-2004
Howard Hughes Medical Institute
2003
In bacteria, the majority of exported proteins are translocated by Sec system, which recognizes signal sequence a preprotein and uses ATP proton motive force to mediate protein translocation across cytoplasmic membrane. SecA is an essential component this containing molecular motor that facilitates translocation. Here we report three-dimensional structure Mycobacterium tuberculosis . Each subunit homodimer contains “motor” domain domain. The predicts can interact with SecYEG pore function as...
The P1 lysozyme Lyz is secreted to the periplasm of Escherichia coli and accumulates in an inactive membrane-tethered form. Genetic biochemical experiments show that, when released from bilayer, activated by intramolecular thiol-disulfide isomerization, which requires a cysteine its N-terminal SAR (signal-arrest-release) domain. Crystal structures confirm alternative disulfide linkages two forms reveal dramatic conformational differences catalytic Thus, exported endolysin kept three levels...
Zn 2+ binds to the active site of SARS-CoV-2 main protease (Mpro), and inhibits enzyme activity viral replication in vitro .
Abstract The sugar will eventually be exported transporter (SWEET) members in Arabidopsis, AtSWEET11 and AtSWEET12 are the important sucrose efflux transporters that act synergistically to perform distinct physiological roles. These two involved apoplasmic phloem loading, seed filling, level alteration at site of pathogen infection. Here, we performed structural analysis binding pocket using molecular docking followed by rigorous dynamics (MD) simulations. We observed molecule binds inside...
Abstract Supplementary information: http://gene.tn.nic.in/sk/profiles/ Contact: krishna@mrna.tn.nic.in To whom correspondence should be addressed.
In eukaryotes, calcium-binding proteins play a pivotal role in diverse cellular processes, and recent findings suggest similar roles for bacterial at different stages their life cycle. Here, we report the crystal structure of calcium dodecin, Rv0379, from Mycobacterium tuberculosis with dodecameric oligomeric assembly unique motif. Structure sequence analysis were used to identify orthologs Rv0379 ligand-binding specificity.
Abstract Homology based 3D structural model of the immunodominant major surface antigen OmpC from Salmonella typhi, an obligatory human pathogen, was built to understand possible unique conformational features its antigenic loops with respect other immunologically cross reacting porins. The homology on known crystal structures E. coli porins OmpF and PhoE. Structure sequence alignment helped define structurally conserved regions (SCRs). SCR were modelled using coordinates corresponding...
Abstract Sugar will eventually be exported transporters (SWEETs) have been identified as a unique class of sugar efflux in all biological kingdoms. AtSWEET11 and AtSWEET12 Arabidopsis act synergistically to perform distinct physiological roles, particularly apoplasmic phloem loading, seed filling, level alteration at the site pathogen infection. Plasma membrane-localized exclusively facilitate sucrose transport along concentration gradient. This article examines binding pocket using docking...
Abstract The lytic enzyme, endolysin, is encoded by bacteriophages (phages) to destroy the peptidoglycan layer of host bacterial cells. release phage progenies start new infection cycle dependent on cell lysis event. Endolysin DLP12 cryptic prophage a SAR endolysin which retained bacterium presumably due benefit it confers. structure (Id: 4ZPU) determined at 2.4 Å resolution presented here. shows modular nature and organized into distinct structural regions. One monomers has loops active...