A5061760126- Escherichia coli research studies
- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Plant tissue culture and regeneration
- Vibrio bacteria research studies
- Tuberculosis Research and Epidemiology
- Bacteriophages and microbial interactions
- Extracellular vesicles in disease
- Mycobacterium research and diagnosis
- Legume Nitrogen Fixing Symbiosis
- Bacterial Infections and Vaccines
- CRISPR and Genetic Engineering
- Plant-Microbe Interactions and Immunity
- Transgenic Plants and Applications
- Sepsis Diagnosis and Treatment
- Neonatal and Maternal Infections
- Plant Pathogenic Bacteria Studies
- Peptidase Inhibition and Analysis
- Bacterial biofilms and quorum sensing
- Yersinia bacterium, plague, ectoparasites research
- Oral microbiology and periodontitis research
- Sinusitis and nasal conditions
- RNA and protein synthesis mechanisms
- Insect symbiosis and bacterial influences
- Urinary Tract Infections Management
Translational Health Science and Technology Institute
2017-2024
The University of Texas at Austin
2014
The University of Texas Health Science Center at Houston
2002-2013
Harvard University
2008-2010
Madurai Kamaraj University
2001
Bacteria use type IV secretion systems (T4SS) to translocate DNA (T-DNA) and protein substrates across the cell envelope. By transfer immunoprecipitation (TrIP), we recently showed that T-DNA translocates through Agrobacterium tumefaciens VirB/D4 T4SS by forming close contacts sequentially with VirD4 receptor, VirB11 ATPase, inner membrane subunits VirB6 VirB8 and, finally, VirB2 pilin VirB9. Here, TrIP, show nucleoside triphosphate binding site (Walker A motif) mutations do not disrupt...
Summary Agrobacterium tumefaciens transfers oncogenic DNA and effector proteins to plant cells during the course of infection. Substrate translocation across bacterial cell envelope is mediated by a type IV secretion (TFS) system composed VirB proteins, as well VirD4, member large family inner membrane implicated in coupling transfer intermediates machine. In this study, we demonstrate with novel cytological screens – two‐hybrid (C2H) assay bimolecular fluorescence complementation (BiFC)...
Mycobacterium tuberculosis (Mtb) requires the ESX1 specialized protein secretion system for virulence, triggering cytosolic immune surveillance pathways, and priming an optimal CD8+ T cell response. This suggests that might act primarily by destabilizing phagosomal membrane surrounds bacterium. However, identifying primary function of has been difficult because deletion any substrate inhibits all known substrates, thereby abolishing activity. Here we demonstrate EspA forms a disulfide bonded...
The bitopic membrane protein VirB10 of the Agrobacterium VirB/VirD4 type IV secretion system (T4SS) undergoes a structural transition in response to sensing ATP binding or hydrolysis by channel ATPases VirD4 and VirB11. This transition, detectable as change protease susceptibility, is required for DNA substrate passage through translocation channel. Here, we present evidence that engagement with VirB11 also activation VirB10. Several substrates (oncogenic T-DNA plasmids RSF1010 pCloDF13)...
Microbes evolve rapidly by modifying their genomes through mutations or the horizontal acquisition of mobile genetic elements (MGEs) linked with fitness traits such as antimicrobial resistance (AMR), virulence, and metabolic functions. We conducted a multicentric study in India collected different clinical samples for decoding genome sequences bacterial pathogens associated sepsis, urinary tract infections, respiratory infections to understand functional potency AMR its dynamics. Genomic...
Background: Diagnosing sepsis in preterm neonates is a significant challenge, underscoring the urgent need for timely and accurate methods. Serum inflammatory protein signatures show promising potential early precise disease diagnosis. Methods: In this study, cohort of (n=50, 25-35 weeks gestation, female 40%) at time suspicion follow-up were enrolled along with healthy neonates. Based on clinical presentation blood culture or MALDI results, cases categorized into culture-positive...
DivIVA of Bacillus subtilis and FtsZ Escherichia coli were used to target heterologous protein complexes cell division sites E. Agrobacterium tumefaciens. that fused the dimerizing leucine zipper (LZ) domain yeast transcription activator GCN4 directed green fluorescent (GFP) was an LZ sites, resulting in fluorescence patterns identical those observed with DivIVA::GFP FtsZ::GFP. These proteins also targeted VirE1 chaperone VirE2 secretion substrate complex A. Coproduction native or inhibited...
Agrobacterium tumefaciens uses a type IV secretion (T4S) system composed of VirB proteins and VirD4 to deliver oncogenic DNA (T-DNA) protein substrates susceptible plant cells during the course infection. Here, by use Transfer ImmunoPrecipitation (TrIP) assay, we present evidence that mobilizable plasmid RSF1010 (IncQ) follows same translocation pathway through VirB/D4 channel as described previously for T-DNA. The transfer intermediate Osa pSa (IncW), related in sequence FiwA fertility...
To survive and establish its niche, Mycobacterium tuberculosis (Mtb) engages in a steady battle against an array of host defenses barrage antibiotics. Here, we demonstrate that Mtb employs HupB, nucleoid-associated protein (NAP) as key player to simultaneously these two stress-inducing fronts. Typically, NAPs are bacterial survival under wide environmental or host-mediated stresses. report for different macrophage-induced assaults including acidic pH, nutrient depletion, oxidative...
The vir genes of octopine, nopaline, and L,L-succinamopine Ti plasmids exhibit structural functional similarities. However, we observed differences in the interactions between octopine nopaline components. induction an virE(A6)::lacZ fusion (pSM358cd) was 2.3-fold higher strain (A348) than a (C58). Supplementation virG(A6) with pSM358 did not completely restore virE(A6) induction. addition virA(A6) to above increased level almost comparable that strains. In reciprocal analysis,...
The 5th Global Forum on TB Vaccines was held in New Delhi, India from 20 to 23 February 2018. This the largest date with nearly 350 participants more than 30 countries. program included over 60 speakers 12 special, plenary and breakout sessions 72 posters. brought a great sense of momentum excitement field. vaccines are clinical trials, new routes delivery being tested, novel assays biomarker signatures developed, results first prevention infection trial H4:IC31 vaccine candidate BCG...
Extracellular vesicles (EVs) have emerged into a novel vaccine platform, biomarker and nano-carrier for approved drugs. Their accurate detection visualization are central to their utility in varied biomedical fields. Owing the limitations of fluorescent dyes antibodies, here, we describe DNA aptamer as promising tool visualizing mycobacterial EVs vitro. Employing SELEX from large library, identified best-performing that is highly specific binds at nanomolar affinity derived three diverse...
Mycobacterium tuberculosis (Mtb) secretes proteases and peptidases to subjugate its host. Out of sixty plus proteases, atleast three are reported reach host macrophages. In this study, we show that Mtb also delivers a lysyl alanine aminopeptidase, PepN (Rv2467) into macrophage cytosol. Our comparative in silico analysis shows PepNMtb highly conserved across all pathogenic mycobacteria. Non-pathogenic mycobacteria including M. smegmatis (Msm) encode pepN. protein levels both (pathogenic) Msm...
An erratum was issued for: Enrichment of Native and Recombinant Extracellular Vesicles Mycobacteria. The Authors section updated from:
Background: The burden of antimicrobial resistance (AMR) continues to rise at an alarming rate, posing a significant challenge its containment, especially in developing countries, owing the rapid emergenceof bacterial pathogens.Methods: Clinical samples collected different hospitals were inoculated selective media for pathogen isolation and characterization by 16S rRNA gene sequencing. Whole genome sequencing clinical strains (n=203) was performed using MiSeq platform. whole-cell proteome...
Most bacteria, including mycobacteria, generate extracellular vesicles (EVs). Since bacterial EVs (bEVs) contain a subset of cellular components, metabolites, lipids, proteins, and nucleic acids, several groups have evaluated either the native or recombinant versions bEVs for their protective potency as subunit vaccine candidates. Unlike EVs, are molecularly engineered to one more immunogens interest. Over last decade, different explored diverse approaches generating bEVs. However, here, we...