A5062568632- Photosynthetic Processes and Mechanisms
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Glycosylation and Glycoproteins Research
- Photoreceptor and optogenetics research
- DNA Repair Mechanisms
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- MicroRNA in disease regulation
- Protein Structure and Dynamics
- Biofuel production and bioconversion
- Advanced Fluorescence Microscopy Techniques
- Pluripotent Stem Cells Research
- DNA and Nucleic Acid Chemistry
- Enzyme Production and Characterization
- Microbial Metabolic Engineering and Bioproduction
- Light effects on plants
- Algal biology and biofuel production
- RNA Research and Splicing
- Microbial Metabolism and Applications
- Enzyme-mediated dye degradation
- Carbohydrate Chemistry and Synthesis
- Biochemical and Molecular Research
- Plant Molecular Biology Research
- Cancer-related molecular mechanisms research
Jamia Millia Islamia
2019-2024
Bhabha Atomic Research Centre
2023
International Centre for Genetic Engineering and Biotechnology
2022
University of Gothenburg
2013-2020
Abeona Therapeutics (United States)
2018
Wilson College
2018
University of Mumbai
2018
Energy and Resources Institute
2017
Lund University
2017
Shri Venkateshwara University
2016
IL-10 is a key regulator of the immune system that critically determines health and disease. Its expression finely tuned both at transcriptional posttranscriptional levels. Although importance regulation has been previously shown, understanding underlying mechanisms still in its infancy. In this study, using combination bioinformatics molecular approaches, we report microRNA (hsa-miR-106a) regulates expression. The hsa-miR-106a binding site 3' UTR IL10 identified by site-directed mutagenesis...
High-affinity, high-selectivity protein-protein interactions that are critical for cell survival present an evolutionary paradox: How does selectivity evolve when acquired mutations risk a lethal loss of high-affinity binding? A detailed understanding in such complexes requires structural information on weak, noncognate which can be difficult to obtain due their transient and dynamic nature. Using NMR-based docking as guide, we deployed disulfide-trapping strategy complex between the colicin...
MicroRNAs (miRs) regulate immunological pathways in health and disease, a number of miRs have been shown to be altered mouse models asthma. The secretion interleukin-10 (IL-10), an anti-inflammatory cytokine, has defective many inflammatory diseases including We recently demonstrated that miR-106a inhibits IL-10 post-transcriptional manner. In this study, we investigated the effect inhibition mmu-miR106a asthmatic condition find its possible role as therapeutic target. Our vitro experiments...
Abstract Serial femtosecond crystallography is an X-ray free-electron-laser-based method with considerable potential to have impact on challenging problems in structural biology. Here we present diffraction data recorded from microcrystals of the Blastochloris viridis photosynthetic reaction centre 2.8 Å resolution and determine its serial structure 3.5 resolution. Although every microcrystal exposed a dose 33 MGy, no signs X-ray-induced radiation damage are visible this integral membrane...
Neurological disorders have been linked to abnormal excitatory neurotransmission. Perturbations in glutamate cycling can profound impacts on normal activity, lead excitotoxicity and neuroinflammation, induce and/or exacerbate impairments these diseases. Astrocytes play a key role signaling as they both clear from the synaptic cleft house enzymes responsible for conversion glutamine. However, mechanisms regulation of cycling, including main astrocytic transporter amino acid 2 (EAAT2 or GLT-1...
The Y-family DNA polymerase IV or PolIV ( Escherichia coli ) is the founding member of DinB family and known to play an important role in stress-induced mutagenesis. We have determined four crystal structures this enzyme its pre-catalytic state complex with substrate presenting possible template nucleotides that are paired corresponding incoming nucleotide triphosphates. In all structures, Ser42 residue active site forms interactions base moieties incipient Watson–Crick pair. This located...
The development of sensitive and specific diagnostic tools for hepatitis B virus (HBV) C (HCV) remains crucial effective disease management control. In this study, we utilized CRISPR‐Cas12 CRISPR‐Cas13 systems the detection HBV (DNA virus) HCV (RNA virus), respectively. We designed tested multiple guide RNAs (gRNAs) targeting both viruses, confirming successful cleavage target sequences through gel electrophoresis a fluorescent reporter assay. Using optimized gRNAs, developed lateral flow...
Chromatin remodelers are ATP-dependent machines that dynamically alter the chromatin packaging of eukaryotic genomes by assembling, sliding, and displacing nucleosomes. The Chd1 remodeler possesses a C-terminal DNA-binding domain is required for efficient nucleosome sliding believed to be essential sensing length DNA flanking core. structure was recently shown consist SANT SLIDE domain, analogous ISWI family, yet details how recognized were not known. Here we present crystal Saccharomyces...
Recognition of bacterial promoters is regulated by two distinct classes sequence-specific sigma factors, σ(70) or σ(54), that differ both in their primary sequence and the requirement latter for activation via enhancer-bound upstream activators. The σ(54) version controls gene expression response to stress, often mediating pathogenicity. Its activator proteins are members AAA+ superfamily use adenosine triphosphate (ATP) hydrolysis remodel initially auto-inhibited holoenzyme promoter...
Error-prone DNA synthesis in prokaryotes imparts plasticity to the genome allow for evolution unfavorable environmental conditions, and this phenomenon is termed adaptive mutagenesis. At a molecular level, mutagenesis mediated by upregulating expression of specialized error-prone polymerases that generally belong Y-family, such as polypeptide product dinB gene case E. coli. However, unlike coli, it has been seen homologs Mycobacterium tuberculosis are not upregulated under conditions stress....
The kinetic and thermodynamic consequences of intrinsic disorder in protein-protein recognition are controversial. We address this by inducing one partner the high-affinity colicin E3 rRNase domain-Im3 complex (K(d) ≈ 10(-12) M) to become an intrinsically disordered protein (IDP). Through a variety biophysical measurements, we show that single alanine mutation at Tyr507 within hydrophobic core isolated domain causes enzyme IDP (E3 rRNase(IDP)). rRNase(IDP) binds stoichiometrically Im3 forms...