A5018642569- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Clostridium difficile and Clostridium perfringens research
- RNA modifications and cancer
- Bacteriophages and microbial interactions
- Pneumonia and Respiratory Infections
- Viral gastroenteritis research and epidemiology
- Antibiotic Resistance in Bacteria
- Antimicrobial Peptides and Activities
- Endoplasmic Reticulum Stress and Disease
- Infant Nutrition and Health
- Advanced Drug Delivery Systems
- RNA Interference and Gene Delivery
- Pneumocystis jirovecii pneumonia detection and treatment
- Streptococcal Infections and Treatments
- Protein Structure and Dynamics
- Bacterial biofilms and quorum sensing
- Neonatal and Maternal Infections
- Parasitic Infections and Diagnostics
University of Lincoln
2015-2018
National Institute for Biological Standards and Control
2008-2011
Witten/Herdecke University
2008
Institute of Molecular Biology
2008
University of York
2004-2008
The cytotoxin colicin E3 targets the 30S subunit of bacterial ribosomes and specifically cleaves 16S rRNA at decoding centre, thereby inhibiting translation. Although cleavage site is well known, it not clear which step translation inhibited. We studied effects on ribosome functions by analysing individual steps protein synthesis. find that affects predominantly elongation step. inhibitory effect originates from accumulation sequential impaired events, each results in low occupancy A and,...
The kinetic and thermodynamic consequences of intrinsic disorder in protein-protein recognition are controversial. We address this by inducing one partner the high-affinity colicin E3 rRNase domain-Im3 complex (K(d) ≈ 10(-12) M) to become an intrinsically disordered protein (IDP). Through a variety biophysical measurements, we show that single alanine mutation at Tyr507 within hydrophobic core isolated domain causes enzyme IDP (E3 rRNase(IDP)). rRNase(IDP) binds stoichiometrically Im3 forms...
Abstract Colicin E3 is a cytotoxic ribonuclease that specifically cleaves 16S rRNA at the ribosomal A‐site to abolish protein synthesis in sensitive Escherichia coli cells. We have performed extensive mutagenesis of 96‐residue colicin domain (E3 rRNase), assayed mutant colicins for vivo cytotoxicity, and tested corresponding rRNase domains their ability inactivate ribosome function vitro. From 21 alanine mutants, we identified five positions where mutation resulted with no measurable...
AbstractStrains of Clostridium difficile produce toxins A and B that can cause diarrhoea pseudomembranous colitis. Currently, there is no preventative therapy for this infection but antibodies to the provide protection, therefore a toxoid-based vaccine needed. To evaluate thermal stability, lyophilized liquid formulation toxoids were stored at range temperatures 5 weeks. Changes in toxoid structures immune responses an animal model before after incubation period assessed. The structural...