A5031317823- Pneumonia and Respiratory Infections
- Bacterial Infections and Vaccines
- Streptococcal Infections and Treatments
- Influenza Virus Research Studies
- Neonatal and Maternal Infections
- Monoclonal and Polyclonal Antibodies Research
- Blood groups and transfusion
- Antimicrobial Resistance in Staphylococcus
- Hemoglobinopathies and Related Disorders
- Blood disorders and treatments
- Inhalation and Respiratory Drug Delivery
- Toxin Mechanisms and Immunotoxins
- Respiratory viral infections research
- Advanced Drug Delivery Systems
- Immunodeficiency and Autoimmune Disorders
- Advancements in Transdermal Drug Delivery
- Glycosylation and Glycoproteins Research
- Botulinum Toxin and Related Neurological Disorders
- Protein purification and stability
- Platelet Disorders and Treatments
- Helicobacter pylori-related gastroenterology studies
- Microbial infections and disease research
- Immune Cell Function and Interaction
- Transgenic Plants and Applications
- Escherichia coli research studies
National Institute for Biological Standards and Control
2008-2022
Government of the United Kingdom
2015
University of Hertfordshire
2002
Imperial College London
1996-2000
St Mary's Hospital
1993-1994
Abstract Group A Streptococcus (Strep A) is a human-exclusive bacterial pathogen killing annually more than 500,000 patients, and no current licensed vaccine exists. Strep bacteria are highly diverse, but all produce an essential, abundant, conserved surface carbohydrate, the Carbohydrate, which contains rhamnose polysaccharide (RhaPS) backbone. RhaPS validated universal candidate in glycoconjugate prepared by chemical conjugation of native carbohydrate to carrier protein. We engineered...
ABSTRACT Oligosaccharides (OSs) related to the pneumococcal type 14 capsular polysaccharide (Pn14PS) were studied for their ability inhibit binding between anti-PS14 antisera and native PS14. A synthetic tetrasaccharide corresponding repeating unit of Pn14PS, a hexasaccharide mimic, an octasaccharide fragment obtained by Pn14PS depolymerization good inhibitors. CRM197 conjugates mimic prepared using either adipic acid diester or diethyl squarate linkers. The conjugate with chains induced...
Mucosal immunity is considered important for protection against Clostridium difficile infection (CDI). We show that in hamsters immunized with Bacillus subtilis spores expressing a carboxy-terminal segment (TcdA26-39) of C. toxin A, no colonization occurs protected animals when challenged strain 630. In contrast, toxoids showed and remained fully colonized. Along neutralizing toxins, antibodies to TcdA26-39 (but not toxoids), whether raised the recombinant protein or expressed on B. spore...
Group A
ABSTRACT Strep A is a human-exclusive bacterial pathogen killing annually more than 500,000 patients, and no current licensed vaccine exists. bacteria are highly diverse, but all produce an essential, abundant, conserved surface carbohydrate, the Group Carbohydrate, which contains rhamnose polysaccharide (RhaPS) backbone. RhaPS validated universal candidate in glycoconjugate prepared by chemical conjugation of native carbohydrate to carrier protein. We engineered Carbohydratte biosynthesis...
Measurement of IgG antibodies against group B streptococcus (GBS) capsular polysaccharide (CPS) by use a standardized and internationally accepted multiplex immunoassay is important for the evaluation candidate maternal GBS vaccines in order to compare results across studies. A assay also required if serocorrelates protection invasive disease are be established infant sera six predominant serotypes since it would permit comparison serotypes. We undertook an interlaboratory study five...
Polysaccharide-protein conjugates as vaccines have proven to be very effective in preventing Haemophilus influenzae type b infections industrialized countries. However, cost-effective technologies need developed for increasing the availability of anti-H. countries from developing world. Consequently, vaccine production with partially synthetic antigens is a desirable goal many reasons. They may rigidly controlled purity and effectiveness while at same time being cheap enough that they made...
Summary We have recently provided evidence that IgG antibodies play a role in the destruction of red cells thalassaemia syndromes. In order further to delineate factors involved clearance thalassaemic cells, monocytes 30 Thai patients with β°‐thal/HbE (17 non‐splenectomized and 13 splenectomized) 16 normal controls were examined for their ability bind phagocytose coated anti‐Rh(D). β°‐thal/HbE. mean number attached was approximately 3‐fold greater than ingested 30% higher. Among patients, by...
Serum levels of tumor necrosis factor-alpha (TNF-alpha), interleukin-1alpha (IL-1alpha), and interferon-gamma (IFN-gamma) were estimated by conventional ELISA kits in 60, 42, 58 Thai patients, respectively, with beta-thalassemia HbE found to be above the normal range 13%, 21%, 33% respectively. Using high-sensitivity systems, an additional 10 beta-thal/HbE patients compared 9 controls for concentrations circulating TNF-alpha IL-1beta , 5 but only 1 none controls, showed values ranges. In...
We examined the antibody responses elicited in rats after transcutaneous immunization (TCI) with Haemophilus influenzae type b (Hib)-cross-reacting material (CRM(197)) glycoconjugate vaccine coadministered cholera toxin or mutants of heat-labile enterotoxin Escherichia coli (LTK63 and LTR72) as adjuvants. The was immunogenic, eliciting high to capsular polysaccharide Hib diphtheria toxin. Passively transferred immune serum protected infant against challenge Eagan strain exhibited strong...
Abstract: Serum levels of M–CSF were determined by an ELISA method in 29 and 34 patients with HbH disease (α 1 /α 2 or α /HbCS) β 0 ‐thal/HbE, respectively, 28 haematologically normal subjects five anaemia due to iron deficiency myelodysplasia. In concentrations significantly higher than those the [986 ± 138 1385 133, vs. 500 33 pg/ml (mean SEM); p <0.01, <0.001, respectively]. By contrast, deficiency, within range. correlated inversely mean basal Hb values ( r = –0.39, 0.05 –0.60,...
Haemophilus influenzae type b (Hib) is a major cause of invasive bacterial infection in children that can be prevented by vaccine, but there still uncertainty about its relative importance Asia. This study investigated the age-specific prevalence Hib carriage and molecular epidemiology disease Nepal. Oropharyngeal swabs were collected from Kathmandu, Nepal, 3 different settings: hospital outpatient department (OPD), schools, children's homes. was isolated using antiserum agar plates,...
In 1997, The Gambia introduced three primary doses of Haemophilus influenzae type b (Hib) conjugate vaccine without a booster in its infant immunisation programme along with establishment population-based surveillance on Hib meningitis the West Coast Region (WCR). This was stopped 2002 reported elimination disease. re-established 2008 but again 2010. We aimed to re-establish WCR and continue Basse Health Demographic Surveillance System (BHDSS) east country assess any shifts epidemiology...