A5054924307- SARS-CoV-2 and COVID-19 Research
- Viral Infectious Diseases and Gene Expression in Insects
- RNA Interference and Gene Delivery
- Viral Infections and Vectors
- Reproductive tract infections research
- RNA and protein synthesis mechanisms
- Viral Infections and Immunology Research
- Bacteriophages and microbial interactions
- Virus-based gene therapy research
- SARS-CoV-2 detection and testing
- Viral gastroenteritis research and epidemiology
- Influenza Virus Research Studies
- Viral Infections and Outbreaks Research
- COVID-19 epidemiological studies
- Immunotherapy and Immune Responses
- Atherosclerosis and Cardiovascular Diseases
- CRISPR and Genetic Engineering
- Vaccine Coverage and Hesitancy
- Animal Disease Management and Epidemiology
- Animal Virus Infections Studies
- Advanced biosensing and bioanalysis techniques
- Cytomegalovirus and herpesvirus research
- Virology and Viral Diseases
- Protein purification and stability
- Vector-Borne Animal Diseases
University of Sheffield
2021-2025
Imperial College London
2016-2025
National Public Health and Medical Officer Service
2018-2024
Semmelweis University
2001-2024
Association des Operateurs Postaux Publics Europeens
2024
Health Center
2024
Orszagos Kornyezetegeszsegugyi Intezet
2020-2021
Centers for Disease Control and Prevention
2013-2020
German Cancer Research Center
2017-2018
Heidelberg University
2017-2018
Analysis of 179 new Ebola virus sequences from patient samples collected in Guinea between March 2014 and January 2015 shows how different lineages evolved spread West Africa. Miles Carroll colleagues report describe the genetic evolution circulating Africa, based on 2015. Their analysis Africa Sierra Leone, Liberia. is currently witnessing most extensive (EBOV) outbreak so far recorded1,2,3. Until now, there have been 27,013 reported cases 11,134 deaths. The origin thought to a zoonotic...
To overcome pandemics, such as COVID-19, vaccines are urgently needed at very high volumes. Here we assess the techno-economic feasibility of producing RNA for demand associated with a global vaccination campaign. Production process performance is assessed three messenger (mRNA) and one self-amplifying (saRNA) vaccines, all currently under clinical development, well hypothetical next-generation saRNA vaccine. The impact key design operation uncertainties on production was assessed. vaccine...
Abstract Overcoming pandemics, such as the current Covid‐19 outbreak, requires manufacture of several billion doses vaccines within months. This is an extremely challenging task given constraints in small‐scale manufacturing for clinical trials, testing timelines involving multiple phases and large‐scale drug substance product manufacturing. To tackle these challenges, regulatory processes are fast‐tracked, rapid‐response platform technologies used. Here, we evaluate progress, challenges...
Lipid nanoparticles (LNPs) are the leading technology for RNA delivery, given success of Pfizer/BioNTech and Moderna COVID-19 mRNA (mRNA) vaccines, small interfering (siRNA) therapies (patisiran). However, optimization LNP process parameters compositions larger payloads such as self-amplifying (saRNA), which can have complex secondary structures, not been carried out. Furthermore, interactions between parameters, critical quality attributes (CQAs), function, protein expression cellular...
Abstract Rapid-response vaccine production platform technologies, including RNA vaccines, are being developed to combat viral epidemics and pandemics. A key enabler of rapid response is having quality-oriented disease-agnostic manufacturing protocols ready ahead outbreaks. We the first apply Quality by Design (QbD) framework enhance rapid-response against known future pathogens. This QbD aims support development consistent safe efficacious integrating a novel qualitative methodology...
mRNA technology has recently demonstrated the ability to significantly change timeline for developing and delivering a new vaccine from years months. The potential of rapid development been highlighted by successful approval two vaccines COVID-19. Importantly, this RNA-based approach holds promise treatments beyond infectious diseases, e.g., cancer, metabolic disorders, cardiovascular conditions, autoimmune diseases. There is currently significant demand improved manufacturing processes...
SARS-CoV-2, the virus responsible for current COVID-19 pandemic, displays a corona-shaped layer of spikes which play fundamental role in infection process. Recent structural data suggest that possess orientational freedom and ribonucleoproteins segregate into basketlike structures. How these features regulate dynamic mechanical behavior native virion are yet unknown. By imaging mechanically manipulating individual, SARS-CoV-2 virions with atomic force microscopy, here, we show their surface...
The transition to energy systems with a high share of renewable depends on the availability technologies that can connect physical distances or bridge time differences between supply and demand points. This study focuses storage due their expected role in liberating sector from fossil fuels facilitating penetration intermittent sources. performance 27 alternatives is compared considering sustainability aspects by means data envelopment analysis. To this end, are first classified into two...
Background —Studies have suggested that the prevalence of antibodies against heat-shock proteins (HSPs), Chlamydia pneumoniae ( Cpn ), and cytomegalovirus (CMV) is associated with coronary artery disease (CAD), but independent or joint effects human (h) HSP60 these pathogens in patients not been fully elucidated. Methods Results —A total 405 subjects (276 CAD 129 control individuals) were tested for serum to hHSP60, , CMV immediate-early-1 (IE1) antigens. Patients also assessed cholesterol,...
Rapid global COVID-19 pandemic response by mass vaccination is currently limited the rate of vaccine manufacturing. This study presents a techno-economic feasibility assessment and comparison three production platform technologies deployed during pandemic: (1) adenovirus-vectored (AVV) vaccines, (2) messenger RNA (mRNA) (3) newer self-amplifying (saRNA) vaccines. Besides assessing baseline performance process, impact key design operational uncertainties on productivity cost these platforms...
Diphyllin (1) and justicidin B (2) are arylnaphthalene lignans with antiviral antiproliferative effects. Compound 1 is also known as an effective inhibitor of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). To evaluate in vitro cytotoxic potency both SARS-CoV-2 -infected cells various cancer cell lines, respectively, were isolated from underground organs Linum austriacum perenne. Two previously undescribed lignans, denominated linadiacin A (3 4), identified. In acidic...
mRNA technology has significantly changed the timeline for developing and delivering a new vaccine from years to months, as demonstrated by development approval of two highly efficacious vaccines...
Background. A unit of the European Mobile Laboratory (EMLab) consortium was deployed to Ebola virus disease (EVD) treatment in Guéckédou, Guinea, from March 2014 through 2015. Methods. The diagnosed EVD and malaria, using RealStar Filovirus Screen reverse transcription–polymerase chain reaction (RT-PCR) kit a malaria rapid diagnostic test, respectively. Results. cleaned EMLab database comprised 4719 samples 2741 cases suspected Guinea. 1231 2178 hospitalized patients (57%) 281 563 who died...
The SARS-CoV-2 pandemic, which started in December 2019, has been posing significant challenges to the health care system worldwide. As pandemic spreads with rapidly increasing number of positive cases, early diagnosis infected patients is crucial successfully limit spread virus. Although real-time reverse-transcription polymerase chain reaction (RT-qPCR) recommended laboratory method diagnose COVID-19 infection, many factors such as availability equipment, reagents and trained personnel...
(1) Background: Crimean-Congo hemorrhagic fever (CCHF) is an emerging tick-borne disease endemic in Africa, Asia, the Middle East, and Balkan Mediterranean regions of Europe. Although no human CCHF cases have been reported, based on vector presence, serological evidence among small vertebrates, general population, Hungary lies within high consensus for potential introduction future infection. Thus, aim our pilot serosurvey was to assess seropositivity cattle sheep as indicator animals virus...