A5078608594- HIV Research and Treatment
- Hepatitis C virus research
- Hepatitis B Virus Studies
- SARS-CoV-2 and COVID-19 Research
- HIV/AIDS drug development and treatment
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Mosquito-borne diseases and control
- Immune Cell Function and Interaction
- HIV/AIDS Research and Interventions
- COVID-19 Clinical Research Studies
- Viral gastroenteritis research and epidemiology
- Liver Disease Diagnosis and Treatment
- Respiratory viral infections research
- Cytomegalovirus and herpesvirus research
- SARS-CoV-2 detection and testing
- Viral Infections and Immunology Research
- Influenza Virus Research Studies
- Herpesvirus Infections and Treatments
- Hepatitis Viruses Studies and Epidemiology
- Malaria Research and Control
- T-cell and B-cell Immunology
- Disaster Response and Management
- Systemic Lupus Erythematosus Research
- COVID-19 epidemiological studies
Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani
2015-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2015-2024
Ospedale Sacro Cuore Don Calabria
2022-2024
Saint Camillus International University of Health and Medical Sciences
2021-2023
University of Siena
2022
Office of Infectious Diseases
2021
State Key Laboratory of Virology
2004-2021
Translational Research Institute
2021
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes
2021
National Tsunami Warning Center
2021
Analysis of 179 new Ebola virus sequences from patient samples collected in Guinea between March 2014 and January 2015 shows how different lineages evolved spread West Africa. Miles Carroll colleagues report describe the genetic evolution circulating Africa, based on 2015. Their analysis Africa Sierra Leone, Liberia. is currently witnessing most extensive (EBOV) outbreak so far recorded1,2,3. Until now, there have been 27,013 reported cases 11,134 deaths. The origin thought to a zoonotic...
A man in his early 30s reported January 2016 a history of fever, asthenia and erythematous rash during stay Haiti. On return to Italy, ZIKV RNA was detected urine saliva 91 days after symptom onset, semen on day 188, six months onset. Our findings support the possibility sexual transmission highlight importance continuing investigate non-vector-borne infection.
Abstract The possible existence of yet undiscovered human tumorigenic viruses is still under scrutiny. development large-scale sequencing technologies, coupled with bioinformatics techniques for the characterization metagenomic sequences, have provided an invaluable tool detection unknown, infectious, agents, as demonstrated by several recent studies. However, discoveries novel possibly associated tumorigenesis are scarce at best. Here, we apply a rigorous workflow to investigate in depth...
Focal segmental glomerulosclerosis is a kidney disease that manifested as the nephrotic syndrome. It often resistant to glucocorticoid therapy and progresses end-stage renal in 50 70% of patients. Genetic studies have shown familial focal podocytes, which are major components glomerular filtration barrier. However, molecular cause over half cases primary unknown, effective treatments been elusive.
SARS-CoV-2 is associated with a 3.4% mortality rate in patients severe disease. The pathogenesis of cases remains unknown. We performed an in-depth prospective analysis immune and inflammation markers two COVID-19 disease from presentation to convalescence. Peripheral blood 18 SARS-CoV-2-infected patients, 9 mild disease, was obtained at admission analyzed for T-cell activation profile, myeloid-derived suppressor cells (MDSCs) cytokine profiles. MDSC functionality tested vitro. In four...
Chronic liver damage leads to pathological accumulation of ECM proteins (liver fibrosis). Comprehensive characterization the human molecular composition is essential for gaining insights into mechanisms disease. To date, studies remodeling in diseases have been hampered by unavailability purified ECM. Here, we developed a decellularization method purify scaffolds from tissues. Histological and electron microscopy analyses demonstrated that scaffolds, devoid plasma cellular components,...
Whole genome sequences of SARS-CoV-2 obtained from two patients, a Chinese tourist visiting Rome and an Italian, were compared with Europe elsewhere. In phylogenetic tree, the Italian patient's sequence clustered Germany while tourist's other European sequences. Some additional in tree segregated outside clusters containing patients' This suggests multiple introductions or virus evolution during circulation.
A novel coronavirus (SARS-CoV-2) has been identified as the causative pathogen of an ongoing outbreak respiratory disease, now named COVID-19. Most cases and sustained transmission occurred in China, but travel-associated have reported other countries, including Europe Italy. Since symptoms are similar to infections, differential diagnosis travellers arriving from countries with wide-spread COVID-19 must include more common infections such influenza tract diseases.
The pathogenesis of SARS-CoV-2 remains to be completely understood, and detailed cellular cytopathic effects requires definition. We performed a comparative ultrastructural study SARS-CoV-1 infection in Vero E6 cells lungs from deceased COVID-19 patients. induces rapid death associated with profound changes cells. Type II pneumocytes lung tissue showed prominent altered features numerous vacuoles swollen mitochondria presence abundant lipid droplets. accumulation lipids was the most striking...
Severe acute respiratory syndrome (SARS) is a newly emerging, highly transmissible and fatal disease caused by previously unknown coronavirus (SARS-CoV). Existing in non-identified animal reservoirs, SARS-CoV continues to represent threat humans because there no effective specific antiviral therapy for infections. Objectives Starting from the observation that cyclopentenone cyclooxygenase (COX) metabolites are active against several RNA viruses, we investigated effect of COX inhibitor...
The spontaneous induced release of interferon gamma (IFN gamma) by cultured intestinal lamina propria lymphocytes was investigated in patients with Crohn's disease. In contrast to normal lymphocytes, from these spontaneously released IFN and seemed contain their cytoplasm. Autologous peripheral did not gamma. When stimulated inducers disease tissue showed an increase 24 hours after induction no appreciable further over the next two days culture, while control cells, either or intestinal,...
Direct population sequencing and reverse hybridization (line probe assay [LiPA])-based methods are the most common for detecting hepatitis B virus (HBV) drug resistance mutations, although only mutations present in viral quasispecies with a prevalence of > or =20% can be detected by sequencing, known LiPA. Massively parallel ultradeep pyrosequencing (UDPS; GS FLX platform) was used to analyze HBV transcriptase (RT) S antigen (HBsAg) from five drug-naive patients eight drug-resistant...
On 10 September 2011, a patient in his 50s was admitted to hospital Ancona, Italy, after six days of high fever and no response antibiotics. West Nile virus (WNV) infection suspected tests determine the aetiology were inconclusive. 20 September, WNV-specific IgM IgG antibodies detected patient’s serum. Genomic sequencing viral isolate showed that belonged WNV lineage 2.
Epidemiological, virological and pathogenetic characteristics of SARS-CoV-2 infection are under evaluation. A better understanding the pathophysiology associated with COVID-19 is crucial to improve treatment modalities develop effective prevention strategies. Transcriptomic proteomic data on host response against still have anecdotic character; currently available from other coronavirus infections therefore a key source information.We investigated selected molecular aspects three human...
Variability in the performance of nucleic acid amplification technology (NAT)-based assays presents a significant problem diagnosis and management human cytomegalovirus (HCMV) infections. Here we describe collaborative study to evaluate suitability candidate reference materials harmonize HCMV viral load measurements wide range NAT assays. Candidate comprised lyophilized Merlin virus, liquid AD169 purified DNA cloned into bacterial artificial chromosome. laboratory mean concentrations...
Abstract The immunological mechanisms underlying the clinical presentation of SARS-CoV-2 infection and those influencing disease outcome remain to be defined. Myeloid-derived suppressor cells (MDSC) have been described highly increased during COVID-19, however, their role remains elusive. We performed an in depth analysis MDSC 128 infected patients. Polymorphonuclear (PMN)-MDSC expanded particular patients who required intensive care treatments, correlated with IL-1β, IL-6, IL-8, TNF-α...