A5046391214- HIV/AIDS drug development and treatment
- HIV Research and Treatment
- Hepatitis C virus research
- HIV/AIDS Research and Interventions
- HIV-related health complications and treatments
- Pharmacological Effects and Toxicity Studies
- Poxvirus research and outbreaks
- COVID-19 Clinical Research Studies
- Herpesvirus Infections and Treatments
- Antibiotics Pharmacokinetics and Efficacy
- Pneumocystis jirovecii pneumonia detection and treatment
- Drug Transport and Resistance Mechanisms
- Immune Cell Function and Interaction
- Renal Transplantation Outcomes and Treatments
- Chronic Lymphocytic Leukemia Research
- T-cell and B-cell Immunology
- Bacillus and Francisella bacterial research
- Cytomegalovirus and herpesvirus research
- Diabetes Treatment and Management
- Pharmacogenetics and Drug Metabolism
- Pregnancy and Medication Impact
- Muscle and Compartmental Disorders
- Analytical Chemistry and Chromatography
- Animal Disease Management and Epidemiology
- Influenza Virus Research Studies
Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani
2014-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2011-2022
Center for Prevention and Treatment of Infections
2010-2015
National Institutes of Health
2013
Abstract The immunological mechanisms underlying the clinical presentation of SARS-CoV-2 infection and those influencing disease outcome remain to be defined. Myeloid-derived suppressor cells (MDSC) have been described highly increased during COVID-19, however, their role remains elusive. We performed an in depth analysis MDSC 128 infected patients. Polymorphonuclear (PMN)-MDSC expanded particular patients who required intensive care treatments, correlated with IL-1β, IL-6, IL-8, TNF-α...
Abstract Background Remdesivir is a prodrug of the nucleoside analogue GS-441524 and under evaluation for treatment SARS-CoV-2-infected patients. Objectives To evaluate pharmacokinetics remdesivir in plasma, bronchoalveolar aspirate (BAS) CSF two critically ill COVID-19 Methods was administered at 200 mg loading dose on first day followed by 12 days 100 Blood samples were collected immediately after (C0) 1 (C1) 24 h (C24) intravenous administration 3 until 9. BAS Days 4, 7 9 from both...
The first evidence that Orthopoxvirus induced the expansion in vivo and recall of effector innate Vδ2 T-cells was described a macaque model. Although, an engagement αβ specific response patients infected with human monkeypox (Mpox) demonstrated, little is known about role γδ during Mpox infection. IFN-γ-producing resistance to poxviruses may key inducing protective type 1 memory immunity. We analyzed kinetics T-cell from acute phase up three months after Fourteen MSM subjects (5 PWH, 35.7%)...
Tuberculosis (TB) is still the principal cause of death caused by a single infectious agent, and balance between bacillus host defense mechanisms reflects different manifestations pathology. The aim this work was to study role myeloid-derived suppressor cells (MDSCs) during active pulmonary tuberculosis at site infection. We observed an expansion MDSCs in lung blood patients with TB, which are correlated enhanced amount nitric oxide plasma. also found that these have remarkable ability...
Avian influenza virus (H5N1) can be transmitted to humans, resulting in a severe or fatal disease. The aim of this study was evaluate the immune cross-reactivity between human and avian strains healthy donors vaccinated for seasonal A (H1N1)/(H3N2). small frequency CD4 T cells specific subtype H5N1 detected several persons at baseline, vaccine administration enhanced such reactive cells. We also observed that vaccination is able raise neutralizing immunity against large number donors. No...
Objective: γδ T cells bearing the Vγ9Vδ2 T-cell receptor exert many antiviral effector functions in humans, including release of anti-HIV factors and direct cytotoxicity against virus-infected cells. Moreover, they are known to activate dendritic cells, improving antigen presentation function. After HIV infection, number reactivity rapidly affected decrease upon disease progression. Bisphosphonate drugs such as zoledronic acid (Zol), used treat bone diseases, have been shown induce vivo,...
Although it is well known that antiretroviral drugs (ARVs) across the placenta in different extents, few data are available concerning impact of transplacental passage ARVs on newborn outcome. The aim this study to evaluate diffusion and clinical assessment newborn. Mother cord lopinavir, nelfinavir, atazanavir nevirapine plasma levels were determined by high-performance liquid chromatography. Newborn gestational age, weight, Apgar score recorded. Cord-to-mother ratio (C:M) was calculated...
Drug metabolism via the cytochrome P450 (CYP450) system has emerged as an important determinant in occurrence of several drug interactions (adverse reactions, reduced pharmacological effect, toxicities). In particular, CYP3A4 and CYP3A5 (interacting with more than 60% licensed drugs) exhibit most individual variations gene expression, mostly caused by single nucleotide polymorphisms (SNPs) within regulatory region genes which might affect level enzyme production. this study, we sought to...
Abstract Therapeutic drug monitoring is pivotal to improve the management of HIV infection. Here, a new HPLC–UV method quantify simultaneously maraviroc and raltegravir levels in human plasma reported. Remarkably, this first for determination plasma. The volume sample was 600 μL. This involved automated solid‐phase extraction with Oasis HLB Cartridge 1 cc (30 mg divinylbenzene N ‐vinylpyrrolidone) evaporation water bath under nitrogen stream. extracted samples were reconstituted 200 μL 50/50...
Sildenafil is increasingly used for the therapy of pulmonary arterial hypertension (PAH) in HIV infected patients. However, concerns exist about pharmacokinetic interactions between sildenafil and protease inhibitors (PI); particular, ritonavir has been shown to increase AUC Cmax by several folds. The aim our study was determine plasma levels PI two patients with PAH treated antiretroviral including ritonavir-boosted PI. Our both experienced above 500 ng/mL; however, they did not report any...
A rapid ART initiation approach can be beneficial in people with advanced HIV disease, consideration of their high morbidity and mortality. The aim our study was to evaluate the feasibility, efficacy safety start BIC/FTC/TAF this setting.
Abstract Therapeutic drug monitoring allows to determine the best dosage regimen adapted each patient optimizing therapeutic benefits, while minimizing risk for side effects. Here, first methodological approach based on matrix‐assisted laser desorption/ionization source equipped with tandem time‐of‐flight (MALDI‐TOF/TOF) mass spectrometry determination of antituberculosis (anti‐TB) drugs ethambutol, pyrazinamide, rifampicin, and streptomycin concentration in plasma tuberculosis‐infected...
Abstract Ebola virus, an enveloped is the cause of largest and most complex virus disease (EVD) outbreak in West Africa. Blood or body fluids infected person may represent a biohazard to laboratory workers. Laboratory tests containing specimens should be conducted referral centres at biosafety level 4, but based on severity clinical symptoms, basic laboratories might required execute urgent for patients suspected EVD. The aim this work was compare analytical performances when Triton X-100,...
The case of a 32-year-old Caucasian female with multi-drug resistant HIV-1 subtype B infection treated salvage regimen including maraviroc, raltegravir, etravirine and unboosted saquinavir who started atovaquone/proguanil prophylaxis, is reported. potential interactions between these anti-retroviral drugs are investigated. Pharmacokinetic analyses documented marked increase in plasma concentrations (+55% +274%, respectively), but not raltegravir maraviroc concentrations. evidence that...
Monitoring of tacrolimus (TAC) concentrations in transplanted patients is necessary to ensure effective immunosuppression and avoid adverse side effects. The fully automated analysis TAC by the affinity column-mediated immunoassay (ACMIA), which does not require a precipitation step, may represent an efficient alternative liquid chromatography-tandem mass spectrometry (LC-MS/MS), including clinically urgent situation. aim this work was compare analytical performances ACMIA with those...
Sildenafil and bosentan are increasingly used for the treatment of pulmonary arterial hypertension (PAH) in HIV-infected patients. However, concerns exist about pharmacokinetic interactions among sildenafil, antiretroviral drugs, including protease inhibitors (PI). We describe here case an patient with PAH, who was co-administered 125 mg twice daily sildenafil 40 three times per day, together a ritonavir-boosted PI-based therapy; plasma levels bosentan, N-desmethylsildenafil, PI were...