A5078853219- HIV/AIDS Research and Interventions
- Cytomegalovirus and herpesvirus research
- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Herpesvirus Infections and Treatments
- Adolescent Sexual and Reproductive Health
- Reproductive tract infections research
- Viral-associated cancers and disorders
- Parvovirus B19 Infection Studies
- Antifungal resistance and susceptibility
- Respiratory viral infections research
- Bacterial Identification and Susceptibility Testing
- Polyomavirus and related diseases
- Biosensors and Analytical Detection
- Fungal Infections and Studies
- SARS-CoV-2 detection and testing
- Syphilis Diagnosis and Treatment
- Viral Infections and Immunology Research
- Hepatitis C virus research
- Molecular Biology Techniques and Applications
- HIV, Drug Use, Sexual Risk
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 and COVID-19 Research
- Hepatitis B Virus Studies
- Food Security and Health in Diverse Populations
Brown University
2016-2025
John Brown University
2022
Rhode Island Hospital
2014-2018
Providence College
2014-2018
Emory University
2005-2014
Miriam Hospital
1997-2014
Texas Tech University
2014
University of South Carolina
2014
Johns Hopkins University Center for AIDS Research
2006-2013
Vanderbilt University
2013
Invasive fungal diseases (IFDs) remain important causes of morbidity and mortality. The consensus definitions the Infectious Diseases Group European Organization for Research Treatment Cancer Mycoses Study have been immense value to researchers who conduct clinical trials antifungals, assess diagnostic tests, undertake epidemiologic studies. However, their utility has not extended beyond patients with cancer or recipients stem cell solid organ transplants. With newer techniques available, it...
Virus-specific CD4 + T helper lymphocytes are critical to the maintenance of effective immunity in a number chronic viral infections, but characteristically undetectable human immunodeficiency virus–type 1 (HIV-1) infection. In individuals who control viremia absence antiviral therapy, polyclonal, persistent, and vigorous HIV-1–specific cell proliferative responses were present, resulting elaboration interferon-γ β chemokines. persons with infection, p24 inversely related load. Strong also...
Despite recent advances, cytomegalovirus (CMV) infections remain one of the most common complications affecting solid organ transplant recipients, conveying higher risks complications, graft loss, morbidity, and mortality. Research in field development prior consensus guidelines supported by The Transplantation Society has allowed a more standardized approach to CMV management. An international multidisciplinary panel experts was convened expand revise evidence expert opinion-based on...
Cytomegalovirus (CMV) continues to be one of the most common infections after solid-organ transplantation, resulting in significant morbidity, graft loss, and adverse outcomes. Management CMV varies considerably among transplant centers but has been become more standardized by publication consensus guidelines Infectious Diseases Section The Transplantation Society. An international panel experts was reconvened October 2012 revise expand evidence expert opinion-based on management, including...
Quantitative real-time PCR (QRT-PCR) has been widely implemented for clinical viral load testing, but a lack of standardization and relatively poor precision have hindered its usefulness. Digital offers highly precise, direct quantification without requiring calibration curve. Performance characteristics were compared to those droplet digital (ddPCR) cytomegalovirus (CMV) testing. Tenfold serial dilutions the World Health Organization (WHO) National Institute Standards Technology (NIST) CMV...
Abstract IDSA Disclaimer As of the time this publication, updates have been made to IDSA’s Guidelines on Diagnosis COVID-19. For most updated version these guidelines, please go https://www.idsociety.org/covid19guidelines. Background Accurate molecular diagnostic tests are necessary for confirming a diagnosis coronavirus disease 2019 (COVID-19). Direct detection severe acute respiratory syndrome 2 (SARS-CoV-2) nucleic acids in tract specimens informs patient, healthcare institution, and...
BackgroundThe availability of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) serologic testing has rapidly increased. Current assays use a variety technologies, measure different classes immunoglobulin or combinations, and detect antibodies directed against portions the virus. The overall accuracy these tests, however, not been well defined. Infectious Diseases Society America (IDSA) convened an expert panel to perform systematic review disease 2019 (COVID-19) serology...
Abstract Background Accurate molecular diagnostic tests are necessary for confirming a diagnosis of coronavirus disease 2019 (COVID-19). Direct detection severe acute respiratory syndrome 2 (SARS-CoV-2) nucleic acids in tract specimens informs patient, healthcare institution and public health level decision-making. The numbers available SARS-CoV-2 acid rapidly increasing, as is the COVID-19 literature. Thus, Infectious Diseases Society America (IDSA) recognized significant need frequently...
To assess interlaboratory variability in qualitative and quantitative cytomegalovirus (CMV) viral load (VL) testing, we distributed a panel of samples to 33 laboratories the USA, Canada Europe who performed testing using commercial reagents (n = 17) or laboratory-developed assays 18). The included two negatives, seven constructed from purified CMV nucleocapsids plasma (2.0-6.0 log(10) copies/mL) three clinical samples. Interlaboratory variation was observed both actual (range, 2.0-4.0...
Background. The early detection of cytomegalovirus (CMV) after liver transplantation may form the basis a preemptive strategy for prevention active CMV disease. Methods. We prospectively analyzed clinical use weekly quantitative polymerase chain reaction-(PCR) based plasma viral load determinations and antigenemia assay predicting development disease in 97 consecutive transplant recipients. Results. occurred 21/97 patients. Using positive cut-off >400 copies/ml plasma, PCR had sensitivity...
<h3>Objectives</h3> To evaluate the efficacy of an intervention to reduce incident sexually transmitted disease (STD) and enhance STD/human immunodeficiency virus (HIV)–preventive behaviors psychosocial mediators. <h3>Design</h3> A randomized controlled trial HIV prevention program. <h3>Setting</h3> Clinic-based sample in Atlanta, Georgia. <h3>Participants</h3> African American adolescent females (N = 715), aged 15 21 years, seeking sexual health services. Participants completed audio...
In a prospective cross-sectional study we quantified HIV viral load within the alveolar macrophage in cohort of healthy HIV-infected subjects who did not have medical comorbidities or smoke cigarettes to determine if proviral DNA was associated with phagocytic immune dysfunction. We enrolled 23 underwent bronchoscopy and bronchoalveolar lavage. Alveolar macrophages were isolated HIV-1 RNA cells using Abbott RealTime Assay. Proviral qualitatively measured modified version assay. Phagocytosis...
Postnatal cytomegalovirus (CMV) infection can cause serious morbidity and mortality in very low-birth-weight (VLBW) infants. The primary sources of postnatal CMV this population are breast milk blood transfusion. current risks attributable to these vectors, as well the efficacy approaches prevent transmission, poorly characterized. To estimate risk transmission from 2 sources: (1) transfusion CMV-seronegative leukoreduced (2) maternal milk. A prospective, multicenter birth-cohort study was...
While viral load testing has gained widespread acceptance, a primary limitation remains the variability of results, particularly between different laboratories. some work demonstrated importance standardized quantitative control material in reducing this variability, little been done to explore other important factors molecular amplification process. Results 185 laboratories enrolled College American Pathologists (CAP) 2009 proficiency (PT) survey (VLS) were examined. This included 165 labs...
Background. Interassay harmonization of cytomegalovirus (CMV) DNA measurement is important for infection management. Uncertainty exists regarding the result achievable in patient plasma samples using quantitative polymerase chain reaction (qPCR) assays with calibrators now traceable to First World Health Organization International Standard (IS) CMV DNA. Method. Serial dilutions IS and a blinded panel 40 genotyped DNA–positive pooled 10 negative were tested by 6 laboratories qPCR calibrated...
Cytomegalovirus (CMV) is an important cause of morbidity and mortality in transplant patients, typically monitored using laboratory-developed quantitative molecular assays. Clinicians who use CMV DNA testing should be aware a number aspects that will aid decision making while managing disease their patients. These include (1) the specimen type used (whole blood or plasma), (2) limit detection quantification chosen by clinical laboratory, (3) linear range assay, (4) reproducibility assay...
Blood cultures are approximately 50% sensitive for diagnosing invasive candidiasis. The T2Candida nanodiagnostic panel uses T2 magnetic resonance and a dedicated instrument to detect Candida directly within whole blood samples. Patients with albicans, glabrata, parapsilosis, tropicalis, or krusei candidemia were identified at 14 centers using diagnostic (dBCs). Follow-up samples collected concurrently testing by companion (cBCs). results reported qualitatively C. albicans/C. glabrata/C....
The clinical signs and symptoms of acute respiratory tract infections (RTIs) are not pathogen specific. Highly sensitive specific nucleic acid amplification tests have become the diagnostic reference standard for viruses, translation bacterial assays from basic research to routine practice represents an exciting advance in medicine. Most recently, molecular diagnostics played essential role global health response novel coronavirus pandemic. How best use newer RTI combination with judgment...