Roman Wölfel
A5036528913- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- SARS-CoV-2 detection and testing
- Viral Infections and Outbreaks Research
- COVID-19 Clinical Research Studies
- Vector-Borne Animal Diseases
- COVID-19 epidemiological studies
- Bacteriophages and microbial interactions
- Bacillus and Francisella bacterial research
- Vector-borne infectious diseases
- Biosensors and Analytical Detection
- Animal Virus Infections Studies
- Fire effects on ecosystems
- Disaster Response and Management
- Yersinia bacterium, plague, ectoparasites research
- Mosquito-borne diseases and control
- Herpesvirus Infections and Treatments
- Poxvirus research and outbreaks
- Viral gastroenteritis research and epidemiology
- vaccines and immunoinformatics approaches
- Virus-based gene therapy research
- Microbial infections and disease research
- Zoonotic diseases and public health
- Hepatitis B Virus Studies
- Advanced biosensing and bioanalysis techniques
German Center for Infection Research
2013-2025
Czech Academy of Sciences, Institute of Microbiology
2006-2024
Institut für Mikrobiologie der Bundeswehr
2015-2024
Universität der Bundeswehr München
2015-2024
Military Hospital of Tunis
2021
Mali-Folkecenter
2019
European Molecular Biology Laboratory
2014-2019
Bernhard Nocht Institute for Tropical Medicine
2007-2016
Bundeswehr Medical Service Academy
2012-2015
Walter Reed Army Institute of Research
2014
In December, 2019, the newly identified severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in Wuhan, China, causing COVID-19, a disease presenting with fever, cough, and often pneumonia. WHO has set strategic objective to interrupt spread of SARS-CoV-2 worldwide. An outbreak Bavaria, Germany, starting at end January, 2020, provided opportunity study transmission events, incubation period, secondary attack rates.
Ebola virus disease (EVD) is a highly lethal condition for which no specific treatment has proven efficacy. In September 2014, while the outbreak was at its peak, World Health Organization released short list of drugs suitable EVD research. Favipiravir, an antiviral developed severe influenza, one these. late conditions starting randomized trial were not fulfilled two reasons. One perception that, given high number patients presenting simultaneously and very mortality rate disease, it...
Coronavirus disease 2019 (COVID-19) is an acute respiratory tract infection that emerged in late 1,2 . Initial outbreaks China involved 13.8% cases with severe-, and 6.1% critical courses 3 This severe presentation corresponds to the usage of a virus receptor expressed predominantly lung 2,4 By causing early onset symptoms, this same tropism thought have determined pathogenicity but also aided control syndrome (SARS) 2003 5 However, there are reports COVID-19 mild upper suggesting potential...
Abstract Crimean-Congo hemorrhagic fever (CCHF) is a highly contagious viral tick-borne disease with case-fatality rates as high 50%. We describe collaborative evaluation of the characteristics, performance, and on-site applicability serologic molecular assays for diagnosis CCHF. evaluated ELISA, immunofluorescence, quantitative reverse transcription PCR, low-density macroarray detection CCHF virus using precharacterized archived patient serum samples. Compared results local, in-house...
Abstract Background Monkeypox is a zoonotic orthopoxvirus infection endemic in central and western Africa. In May 2022, human monkeypox infections including human-to-human transmission were reported multi-country outbreak Europe North America. Case presentations Here we present the first two cases of humans diagnosed Germany. We clinical virological findings, detection virus DNA blood semen. The presentation medical history our patients suggest close physical contact during sexual...
To end the largest known outbreak of Ebola virus disease (EVD) in West Africa and to prevent new transmissions, rapid epidemiological tracing cases contacts was required. The ability quickly identify unknown sources chains transmission is key ending EVD epidemic even greater importance context recent reports (EBOV) persistence survivors. Phylogenetic analysis complete EBOV genomes can provide important information on source any infection. A local deep sequencing facility established at...
Summary The global emergence of SARS-CoV-2 urgently requires an in-depth understanding molecular functions viral proteins and their interactions with the host proteome. Several individual omics studies have extended our knowledge COVID-19 pathophysiology 1–10 . Integration such datasets to obtain a holistic view virus-host define pathogenic properties is limited by heterogeneity experimental systems. We therefore conducted concurrent multi-omics study SARS-CoV. Using state-of-the-art...
By January, 2016, all known transmission chains of the Ebola virus disease (EVD) outbreak in west Africa had been stopped. However, there is concern about persistence reproductive tract men who have survived EVD. We aimed to use biostatistical modelling describe dynamics RNA load seminal fluid, including clearance parameters.In this longitudinal study, we recruited discharged from three treatment units Guinea between January and July, 2015. Participants provided samples fluid at follow-up...
Quantitative serological assays detecting response to SARS-CoV-2 are needed quantify immunity. This study analyzed the performance and correlation of two quantitative anti-S1 in oligo-/asymptomatic individuals from a population-based cohort.In total, 362 plasma samples (108 with reverse transcription-polymerase chain reaction [RT-PCR]-positive pharyngeal swabs, 111 negative controls, 143 positive serology without confirmation by RT-PCR) were tested (Euroimmun Anti-SARS-CoV-2 QuantiVac...
Given the large number of mild or asymptomatic SARS-CoV-2 cases, only population-based studies can provide reliable estimates magnitude pandemic. We therefore aimed to assess sero-prevalence in Munich general population after first wave For this purpose, we drew a representative sample 2994 private households and invited household members 14 years older complete questionnaires blood samples. seropositivity was defined as Roche N pan-Ig ≥ 0.4218. adjusted prevalence for sampling design,...
Neutrophils are key players during host defense and sterile inflammation. Neutrophil dysfunction is a characteristic feature of the acquired immunodeficiency kidney disease. We speculated that impaired renal clearance intrinsic purine metabolite soluble uric acid (sUA) may account for neutrophil dysfunction. Indeed, hyperuricemia (HU, serum UA 9-12 mg/dL) related or unrelated to significantly diminished adhesion extravasation in mice with crystal- coronavirus-related inflammation using...
SARS-CoV-2 infection is accompanied by elevated liver enzymes, and patients with pre-existing conditions experience more severe disease. While it was known that infects human hepatocytes, our study determines the mechanism of infection, demonstrates viral replication spread, highlights direct hepatocyte damage. Viral readily detectable upon primary hepatocytes hepatoma cells ancestral SARS-CoV-2, Delta, Omicron variants. Hepatocytes express receptor ACE2 host cell protease TMPRSS2, knocking...
Abstract We developed a real-time reverse transcription–-PCR that detected 1,164 copies/mL of Crimean-Congo hemorrhagic fever virus per milliliter serum at 95% probability (probit analysis) and was 100% concordant with nested PCR on 63 samples from 31 patients confirmed infection. Infected who died appeared to have higher viral loads; low loads correlated IgG detection.
Background Whole genome sequencing allowed the development of a number high resolution sequence based typing tools for Yersinia (Y.) pestis. The application these methods on isolates from most known foci worldwide and in particular China Former Soviet Union has dramatically improved our understanding population structure this species. In current view, Y. pestis including non or moderate human pathogen subspecies microtus emerged pseudotuberculosis about 2,600 to 28,600 years ago central...
Binary file ES_Abstracts_Final_ECDC.txt matches
Containment strategies and clinical management of coronavirus disease (COVID-19) patients during the current pandemic depend on reliable diagnostic PCR assays for severe acute respiratory syndrome 2 (SARS-CoV-2). Here, we compare 11 different RT-PCR test systems used in seven laboratories Germany March 2020. While most performed well, identified detection problems a commonly assay that may have resulted false-negative results first weeks pandemic.
Abstract Prolonged shedding of infectious SARS-CoV-2 has recently been reported in a number immunosuppressed individuals with COVID-19. Here, we describe the detection high levels replication-competent specimens taken from respiratory tract B-cell depleted patient up to 154 days after initial COVID-19 diagnosis concomitant development mutation rate. In this patient, total 11 nonsynonymous mutations were detected addition Y144 deletion spike protein SARS-CoV-2. Virus evolution studies...