A5053527974- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Vector-borne infectious diseases
- Viral Infections and Outbreaks Research
- Fire effects on ecosystems
- Vector-Borne Animal Diseases
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Poxvirus research and outbreaks
- Herpesvirus Infections and Treatments
- Bacillus and Francisella bacterial research
- Respiratory viral infections research
- COVID-19 Clinical Research Studies
- Virology and Viral Diseases
- Biosensors and Analytical Detection
- Long-Term Effects of COVID-19
- COVID-19 epidemiological studies
- Bacteriophages and microbial interactions
- Pneumonia and Respiratory Infections
- Malaria Research and Control
- Insect and Pesticide Research
- Autoimmune and Inflammatory Disorders Research
- Optimism, Hope, and Well-being
- Advanced biosensing and bioanalysis techniques
- Leptospirosis research and findings
University of Ljubljana
2016-2025
Institute of Oncology Ljubljana
2009-2024
Czech Academy of Sciences, Institute of Microbiology
2016-2024
Sydney Local Health District
2023
University of Helsinki
2023
Helsinki University Hospital
2023
University Medical Center Hamburg-Eppendorf
2023
Universität Hamburg
2023
University of California, Irvine
2022
Ljubljana University Medical Centre
2013-2022
A widespread epidemic of Zika virus (ZIKV) infection was reported in 2015 South and Central America the Caribbean. major concern associated with this is apparent increased incidence microcephaly fetuses born to mothers infected ZIKV. In report, we describe case an expectant mother who had a febrile illness rash at end first trimester pregnancy while she living Brazil. Ultrasonography performed 29 weeks gestation revealed calcifications fetal brain placenta. After requested termination...
Analysis of 179 new Ebola virus sequences from patient samples collected in Guinea between March 2014 and January 2015 shows how different lineages evolved spread West Africa. Miles Carroll colleagues report describe the genetic evolution circulating Africa, based on 2015. Their analysis Africa Sierra Leone, Liberia. is currently witnessing most extensive (EBOV) outbreak so far recorded1,2,3. Until now, there have been 27,013 reported cases 11,134 deaths. The origin thought to a zoonotic...
Abstract Crimean-Congo hemorrhagic fever (CCHF) is a highly contagious viral tick-borne disease with case-fatality rates as high 50%. We describe collaborative evaluation of the characteristics, performance, and on-site applicability serologic molecular assays for diagnosis CCHF. evaluated ELISA, immunofluorescence, quantitative reverse transcription PCR, low-density macroarray detection CCHF virus using precharacterized archived patient serum samples. Compared results local, in-house...
Laboratories are currently witnessing extraordinary demand globally for sampling devices, reagents, consumables, and diagnostic instruments needed timely diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. To meet needs as the pandemic grows, U.S. Food Drug Administration (FDA) recently granted several commercial SARS-CoV-2 tests Emergency Use Authorization (EUA), but manufacturer-independent evaluation data scarce. We performed first fully automated...
Neutralization tests (NT) are the gold standard for detecting and quantifying anti-SARS-CoV-2 neutralizing antibodies (NAb), but their complexity restricts them to research settings or reference laboratories. Antibodies against S protein receptor binding domain (RBD) have been shown confer a activity SARS-CoV-2. Assays quantitatively measuring anti-S1-RBD-SARS-CoV-2 could be of great value NAb screening potential donors convalescent-phase plasma therapy, assessing natural vaccine-induced...
Tick-borne encephalitis (TBE) developed in 3 persons Slovenia who drank raw milk; a fourth person, had been vaccinated against TBE, remained healthy. TBE virus RNA was detected serum and milk of the source goat. Persons TBE-endemic areas should be encouraged to drink only boiled/pasteurized vaccinated.
Tick-borne encephalitis virus (TBEV) is the most important causative agent of arboviral infection in Europe, causing neurologic symptoms. The incidence disease has greatly increased over past decades, and meantime, some changes spatial distribution TBE cases have been observed. Therefore, it to recognize endemic areas, use preventive measures successfully. In this study, rodents from all Slovenia were evaluated as suitable sentinels for TBEV distribution. Rodents four species (Myodes...
Tick-borne encephalitis virus (TBEV) causes one of the most dangerous human neuroinfections in Europe and Asia. To infect neurons it must cross blood-brain-barrier (BBB), presumably also cells adjacent to BBB, such as astrocytes, abundant glial cell type. However, knowledge about viral infection is fragmental. Here we studied whether TBEV infects rat astrocytes. Rats belong an animal group serving a amplifying host. We employed high resolution quantitative fluorescence microscopy investigate...
Abstract Malformations of the fetal CNS, known as microcephaly, have been linked to Zika virus (ZIKV) infection. Here, responses mammalian and mosquito cell lines, in addition primary human astrocytes neurons were studied following infection by ZIKV strains Brazil 2016 (ZIKV-BR), French Polynesia 2013 (ZIKV-FP), Uganda #976 1947 (ZIKV-UG). Viral production, viability, infectivity rate, mobility endocytotic ZIKV-laden vesicles compared. All types (SK-N-SH, Vero E6, C6/36, neurons) released...
Monkeypox virus (MPXV), originally endemic in West Africa (Clade II) and Central I), has recently emerged worldwide reinforced the need for rapid accurate MPXV diagnostics. This review presents critically discusses range of virological methods laboratory diagnosis characterization as well related lessons learned practical experience gained from 2022 Mpox global outbreak. Real-time PCR is currently considered diagnostic gold standard ensures timely confirmation suspected cases based on...
To asses the role of virus load in pathogenesis hemorrhagic fever with renal syndrome, serum Dobrava RNA 46 patients was measured a novel quantitative real-time reverse-transcriptase polymerase chain reacion assay and compared to disease severity. The level viremia, detected 26 patients, ranged from 102−108 copies/mL serum. severe had, on average, higher viral loads than milder course (6.15 vs. 4.67 log10 copies/mL; P = .053). These results suggest that might be associated severity disease.
Hantaviruses, Leptospira spp., and Babesia spp. are rodent-borne pathogens present worldwide. We studied multiple co-infections of small rodents in Croatia with all three pathogens. Twenty-eight Apodemus flavicollis 16 Myodes glareolus were tested for the presence hantavirus RNA by real-time RT-PCR, strains renoculture method DNA PCR. Anti-hantavirus antibodies anti-Leptospira detected serological methods. Very high infection rates each pathogen found A. flavicollis: 20 28 (71%) infected...
Crimean-Congo hemorrhagic fever (CCHF) is an acute, tick borne disease often associated with presentations and high case fatality rate. Kosovo a highly endemic area for CCHF, significant The aim of our study was to determine the prevalence CCHF in Kosovo. We tested 1105 serum samples from healthy population both non-endemic areas country. Our results revealed seroprevalence 4.0% (range 0–9.3%) which comparable other countries. show that correlated incidence each studied municipality. also...
In the past decade, many new paramyxoviruses that do not belong to any of seven established genera in family Paramyxoviridae have been discovered. Amongst them are J-virus (JPV), Beilong virus (BeiPV) and Tailam (TlmPV), three paramyxovirus species found rodents. Based on their similarities, it has suggested these viruses should compose a genus, tentatively called 'Jeilongvirus'.Here we present complete genomes newly discovered paramyxoviruses, one bank vole (Myodes glareolus) from Slovenia...
The tick-borne encephalitis complex contains a number of flaviviruses that share close genetic homology, and are responsible for significant human morbidity mortality with widespread geographical range. Although many members this have been recognised decades, licenced vaccines broad availability only available virus. While virus demonstrated to induce protective immunity, as determined by virus-neutralisation titres, vaccine breakthrough (clinical infection following complete vaccination),...
We report a case of natural infection with severe acute respiratory syndrome coronavirus 2 transmitted from an owner to pet ferret in the same household Slovenia. The had onset gastroenteritis dehydration. Whole-genome sequencing viruses isolated and revealed 2-nt difference.