A5071968852- Viral Infections and Vectors
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- Mosquito-borne diseases and control
- Animal Virus Infections Studies
- Plant Virus Research Studies
- Respiratory viral infections research
- Virology and Viral Diseases
- Genomics and Phylogenetic Studies
- Molecular Biology Techniques and Applications
- Animal Disease Management and Epidemiology
- Diabetes and associated disorders
- Chromosomal and Genetic Variations
- Malaria Research and Control
- Cervical Cancer and HPV Research
- interferon and immune responses
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- Insect symbiosis and bacterial influences
- Viral gastroenteritis research and epidemiology
- Genetic diversity and population structure
- Viral Infections and Immunology Research
- HIV Research and Treatment
Rega Institute for Medical Research
2016-2023
KU Leuven
2016-2023
National and Kapodistrian University of Athens
2019-2023
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2019
University of Arizona
2019
Humboldt-Universität zu Berlin
2019
Freie Universität Berlin
2019
University of Thessaly
2015
In the past decade, many new paramyxoviruses that do not belong to any of seven established genera in family Paramyxoviridae have been discovered. Amongst them are J-virus (JPV), Beilong virus (BeiPV) and Tailam (TlmPV), three paramyxovirus species found rodents. Based on their similarities, it has suggested these viruses should compose a genus, tentatively called 'Jeilongvirus'.Here we present complete genomes newly discovered paramyxoviruses, one bank vole (Myodes glareolus) from Slovenia...
The hepatitis C virus (HCV) is a major human pathogen. Genetically related viruses in animals suggest zoonotic origin of HCV. closest relative HCV found horses (termed equine hepacivirus [EqHV]). However, low EqHV genetic diversity implies relatively recent acquisition by horses, making derivation from unlikely. To unravel the evolutionary history within equid sister species, we analyzed 829 donkeys and 53 mules sampled nine European, Asian, African, American countries molecular serologic...
The need to estimate divergence times in evolutionary histories the presence of various sources substitution rate variation has stimulated a rich development relaxed molecular clock models. Viral studies frequently adopt an uncorrelated model as generic process, but this may impose considerable estimation bias if discrete exists among clades or lineages. For HIV-1 group M, subtypes been shown result inconsistencies time most recent common ancestor estimation. Although calls into question...
As viral genomic imprints in host genomes, endogenous elements (EVEs) shed light on the deep evolutionary history of viruses, ancestral ranges, and ancient viral-host interactions. In addition, they may provide crucial information for calibrating timescales. this study, we conducted a comprehensive silico screening large data set available mammalian genomes EVEs deriving from members family Flaviviridae, an important group viruses including well-known human pathogens, such as Zika, dengue,...
High-throughput sequencing technologies have advanced RNA virus genomics, but recovering viral genomes from mammalian tissues remains challenging due to the predominance of host RNA. We evaluated two metatranscriptomic workflows address these challenges. Our results demonstrate that methods differed significantly in performance, with Method B achieving a 5-fold increase yield and improved integrity over A. These differences resulted recovery 4 complete hepacivirus compared fragmented or...
Here, we identified a novel class of compounds which demonstrated good antiviral activity against dengue and Zika virus infection. These derivatives constitute intermediates in the synthesis indole (ervatamine-silicine) alkaloids share tetracyclic structure, with an piperidine fused to seven-membered carbocyclic ring. Structure-activity relationship studies indicated importance substituent at position C-6 especially presence benzyl ester for cytotoxicity molecules. In addition,...
We report here the complete genome sequence of ruloma virus, a novel paramyxovirus detected in Machangu’s brush-furred rat from Tanzania. Ruloma virus has longest orthoparamyxovirus reported to date and forms sister clade all currently known members genus Jeilongvirus .
In the search for natural reservoirs of hepatitis C virus (HCV), a broad diversity non-human viruses within Hepacivirus genus has been uncovered. However, evolutionary dynamics that shaped and timescale hepaciviruses evolution remain elusive. To gain further insights into origins this genus, we screened large dataset wild mammal samples (n = 1,672) from Africa Asia, generated 34 full-length hepacivirus genomes. Phylogenetic analysis these data together with publicly available genomes...
Abstract Hepatitis C virus (HCV; genus Hepacivirus) represents a major public health problem, infecting about three per cent of the human population. Because no animal reservoir carrying closely related hepaciviruses has been identified, zoonotic origins HCV still remain unresolved. Motivated by recent findings divergent in rodents and plausible African origin genotypes, we have screened large collection small mammals samples from seven sub-Saharan countries. Out 4,303 screened, eighty were...
Recent years have witnessed the discovery of several new viruses belonging to family Arteriviridae, expanding known diversity and host range this group complex RNA viruses. Although pathological relevance these is not always clear, well-studied members Arteriviridae are be important animal pathogens. Here, we report complete genome sequences four arterivirus variants, two putative novel species. These arteriviruses were discovered in African rodents given names Lopma virus Praja virus. Their...
This protocol is used for successful NGS library preparation from total RNA of animal tissue samples. With this method we obtained full-length paramyxovirus genomes using the Illumina sequencing platform. It works most efficiently on viruses possessing a polyA tail, but it can also be any other viral sequences originating
Abstract As viral genomic imprints in host genomes, endogenous elements (EVEs) shed light on the deep evolutionary history of viruses, ancestral ranges, and ancient viral-host interactions. In addition, they may provide crucial information for calibrating timescales. this study, we conducted a comprehensive silico screening large dataset available mammalian genomes EVEs deriving from members family Flaviviridae , an important group viruses including well-known human pathogens. We identified...
Viruses are the cause of a considerable burden to human, animal and plant health, while on other hand playing an important role in regulating entire ecosystems. The power new sequencing technologies combined with tools for processing "Big Data" offers unprecedented opportunities answer fundamental questions virology. Virologists have urgent need virus-specific bioinformatics tools. These developments led formation European Virus Bioinformatics Center, network experts virology who joining...
This protocol can be used for total RNA purification from tissue samples. It includes an intermediate on-column DNase treatment, which results in higher yield and purity. method is mainly optimised to obtain hepacivirus extracts whole-genome sequencing, but it also any viral samples with lower loads.
Abstract Hepatitis C virus (HCV; genus Hepacivirus ) represents a major public health problem, infecting about 3 % of the human population ( ± 185,000,000 people). Because no plausible animal reservoir carrying closely related hepaciviruses has been identified, zoonotic origins HCV still remain elusive. Motivated by recent findings divergent in rodents and African origin genotypes, we have screened comprehensive collection small mammals samples from seven sub-Saharan countries. Out 4,303...
Abstract Phylogenetic studies have contributed to our understanding of the early epidemic onset HIV-1 in Democratic Republic Congo (DRC); however, factors driving its emergence and establishment human populations still remain unresolved. In order determine key aspects successful spread, complete genome data are required from samples representative viral diversity DRC. this study, we established a universal PCR-assay that uses seven different panels primers produce overlapping amplicons...