A5074284350- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Viral Infections and Vectors
- Virology and Viral Diseases
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Mycotoxins in Agriculture and Food
- Autophagy in Disease and Therapy
- Viral Infections and Outbreaks Research
- interferon and immune responses
- Spine and Intervertebral Disc Pathology
- Medical Imaging and Analysis
- Nanoplatforms for cancer theranostics
- Photoacoustic and Ultrasonic Imaging
- Antimicrobial Peptides and Activities
- Enzyme Catalysis and Immobilization
- Wheat and Barley Genetics and Pathology
- Ferroelectric and Negative Capacitance Devices
- Traumatic Brain Injury and Neurovascular Disturbances
- Phagocytosis and Immune Regulation
- Respiratory viral infections research
- Cervical and Thoracic Myelopathy
Institute of Geochemistry
2025
Chinese Academy of Sciences
2017-2025
Nanjing Medical University
2025
Jiangsu Province Hospital
2025
Shaanxi Provincial People's Hospital
2023-2024
Shanxi Medical University
2023-2024
Harbin Normal University
2022-2024
Sichuan University
2023
West China Hospital of Sichuan University
2023
Southwest Forestry University
2010-2023
Autophagy is an essential component of host innate and adaptive immunity. Viruses have developed diverse strategies for evading or utilizing autophagy survival. The response the pathways to virus invasion poorly documented. Here, we report on induction initiated by pathogen receptor HSP90AA1 (heat shock protein 90 kDa α [cytosolic], class A member 1) via AKT-MTOR (mechanistic target rapamycin)-dependent pathway. Transmission electron microscopy confocal revealed that intracellular...
To end the largest known outbreak of Ebola virus disease (EVD) in West Africa and to prevent new transmissions, rapid epidemiological tracing cases contacts was required. The ability quickly identify unknown sources chains transmission is key ending EVD epidemic even greater importance context recent reports (EBOV) persistence survivors. Phylogenetic analysis complete EBOV genomes can provide important information on source any infection. A local deep sequencing facility established at...
Interleukin-2 (IL-2) has an essential role in the expansion and function of CD4+ regulatory T cells (Tregs). Tregs reduce tissue damage by limiting immune response following infection regulate autoreactive effector (Teffs) to prevent autoimmune diseases, such as type 1 diabetes (T1D). Genetic susceptibility T1D causes alterations IL-2 pathway, a finding that supports cellular therapeutic target. Aldesleukin (Proleukin; recombinant human IL-2), which is administered at high doses activate...
Viruses depend on the host cells they infect to provide machinery and substrates for replication. Host are highly dynamic systems that can alter their intracellular environment metabolic behavior, which may be helpful or inhibitory an infecting virus. In this study, we show macrophages, a target cell of murine norovirus (MNV), increase glycolysis upon viral infection, is important early steps in MNV infection. Human noroviruses (hNoV) major cause gastroenteritis globally, causing enormous...
The biodegradation path and mechanism of wood varies depending on diverse fungi tree species, as possess selectivity in degradation versatile components. This paper aims to clarify the actual precise white brown rot effects different species. Softwood (Pinus yunnanensis Cunninghamia lanceolata) hardwood (Populus Hevea brasiliensis) were subjected a biopretreating process by fungus Trametes versicolor, Gloeophyllum trabeum Rhodonia placenta with various conversion periods. results showed that...
Abstract Murine norovirus (MNV) is a positive‐sense, plus‐stranded RNA virus in the Caliciviridae family. It most common pathogen biomedical research colonies. MNV also related to human noroviruses, which cause majority of nonbacterial gastroenteritis worldwide. Like an enteric that replicates intestine and transmitted by fecal‐oral route. murine macrophages dendritic cells culture host. This often used study mechanisms biology, because noroviruses are refractory growth cell culture....
ABSTRACT The Norovirus genus contains important human pathogens, but the role of host pathways in norovirus replication is largely unknown. Murine noroviruses provide opportunity to study cell culture and small animals. nonstructural protein NS1/2 interacts with VAMP-associated A (VAPA), significance NS1/2-VAPA interaction unexplored. Here we report decreased murine VAPA- VAPB-deficient cells. We characterized VAPA detail. was required for efficiency a step(s) viral cycle after entry RNA...
Knowledge of the host factors required for norovirus replication has been hindered by challenges associated with culturing human noroviruses. We have combined proteomic analysis viral translation and complexes a CRISPR screen, to identify infection. The core stress granule component G3BP1 was identified as factor essential efficient murine infection, demonstrating conserved function across Norovirus genus. Furthermore, we show that functions in novel paradigm VPg-dependent initiation,...
Phototherapy and sonodynamic therapy (SDT) are widely used for the synergistic treatment of tumors have received considerable attention. However, an inappropriate tumor microenvironment, including pH, H
ABSTRACT Chicken MDA5 (chMDA5), the sole known pattern recognition receptor for cytoplasmic viral RNA in chickens, initiates type I interferon (IFN) production. Infectious bursal disease virus (IBDV) evades host innate immunity, but mechanism is unclear. We report here that IBDV inhibited antiviral immunity via chMDA5-dependent signaling pathway. infection did not induce efficient production antagonized activity of beta (IFN-β) DF-1 cells pretreated with IFN-α/β. Dual-luciferase assays and...
Viral infections impose major stress on the host cell. In response, pathways can rapidly deploy defence mechanisms by shutting off protein synthesis machinery and triggering accumulation of mRNAs into granules to limit use energy nutrients. Because this threatens viral gene expression, viruses need evade these propagate. Human norovirus is responsible for gastroenteritis outbreaks worldwide. Here we examined how interacts with eIF2α signaling axis controlling translation granules. While...
The gene encoding pyrethroid-hydrolyzing esterase (EstP) from Klebsiella sp. strain ZD112 was cloned into Escherichia coli and sequenced. A sequence analysis of the DNA responsible for estP revealed an open reading frame 1914 bp a protein 637 amino acid residues. No similarities were found by database homology search using nucleotide deduced sequences esterases lipases. EstP heterologously expressed in E. purified. molecular mass native enzyme approximately 73 kDa as determined gel...
Precise investigation and manipulation of dynamic biological processes often requires molecular modulation in a controlled inducible manner. The clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR associated protein 9 (Cas9) has emerged as versatile tool for targeted gene editing transcriptional programming. Here, we designed vigorously optimized series Hybrid drug Inducible CRISPR/Cas9 Technologies (HIT) activation by grafting mutated human estrogen receptor (ERT2)...
Non-point source pollution poses a significant threat to global water security, and risk assessment key area (CSA) identification are critical for its management. While source–sink landscape models widely used non-point evaluation, their application in karst regions is challenged by ecological fragility, shallow soil layers, severe erosion, limiting effectiveness accurately identifying risks CSAs. This study focuses on the Caohai Lake basin southwestern China; it integrates...
To explore the mechanisms of TLR9 from macrophages on mitochondrial apoptosis in cardiomyocytes at early stage sepsis. The vivo and vitro sepsis mice were bone marrow transplantation (BMT) with wild type (WT) or (toll-like receptor 9) knockout (-/- KO) myeloid cells then constructed by cecum ligation puncture (CLP) as experiment cocultured WT TLR9-deficient treated LPS experiment, respectively. Sepsis model performed CLP. expression levels exosome, PI3K/AKT, ERK1/2, inflammatory factors,...
The gene bgaP encoding cold-active beta-galactosidase from a psychrotrophic and halotolerant Planococcus sp. L4 was cloned into Escherichia coli sequenced. A sequence analysis of the DNA responsible for BgaP revealed an open reading frame 2031 bp protein 677 amino acid residues. heterologously expressed in E. purified followed by Ni2+ affinity chromatography. molecular mass native enzyme approximately 156 kDa as determined gel filtration. results sodium dodecyl sulfate-polyacrylamide...
The CRISPR/Cas9 enabled efficient gene editing in an easy and programmable manner. Controlling its activity greater precision is desired for biomedical research potential therapeutic translation. Here, we engrafted the system with a mutated human estrogen receptor (ERT2), which renders it 4-hydroxytamoxifen (4-OHT) inducible access of genome, nuclear export signal (NES), lowers background activity. Tight drug-inducible genome was achieved across several cell types, including embryonic stem...