A5009869157- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Nanoplatforms for cancer theranostics
- Microfluidic and Bio-sensing Technologies
- Acute Myeloid Leukemia Research
- Cancer Cells and Metastasis
- Virus-based gene therapy research
- Cancer, Hypoxia, and Metabolism
- CAR-T cell therapy research
- Single-cell and spatial transcriptomics
- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- Chronic Myeloid Leukemia Treatments
- Acute Lymphoblastic Leukemia research
- Immune Cell Function and Interaction
- Cancer Genomics and Diagnostics
- Cancer Research and Treatments
- Telomeres, Telomerase, and Senescence
- Eosinophilic Disorders and Syndromes
- Phagocytosis and Immune Regulation
- Advanced Nanomaterials in Catalysis
- Monoclonal and Polyclonal Antibodies Research
- Cancer, Stress, Anesthesia, and Immune Response
Nanjing University of Chinese Medicine
2019-2025
Zhejiang Chinese Medical University
2024
The University of Texas MD Anderson Cancer Center
2010-2024
Houston Methodist
2014-2023
Chinese University of Hong Kong, Shenzhen
2023
Hebei Medical University
2022-2023
Zhengzhou University
2023
Second Hospital of Hebei Medical University
2022
Cornell University
2014-2019
Wenzhou Medical University
2017-2019
The CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) nuclease system represents an efficient tool for genome editing and gene function analysis. It consists of two components: single-guide RNA (sgRNA) the enzyme Cas9. Typical sgRNA Cas9 intracellular delivery techniques are limited by their reliance on cell type exogenous materials as well toxic effects cells (for example, electroporation). We introduce optimize a microfluidic membrane deformation...
As the key effector in Hippo pathway, YAP was identified as an oncoprotein whose expression is elevated various human cancers. However, development of potentially therapeutic compounds targeting has been slow and limited. Here, we find that tankyrase inhibitors suppress activity. This effect mediated by anigomotin (AMOT) family proteins. Tankyrases associate with AMOT proteins promote their degradation through E3 ligase RNF146. By antagonizing activity, stabilize proteins, thereby...
Near-infrared (NIR)-light-triggered photothermal therapy (PTT) is usually associated with undesirable damage to healthy organs nearby due the high temperatures (>50 °C) available for tumor ablation. Low-temperature PTT would therefore have tremendous value clinical application. Here, we construct a hypoxia-responsive gold nanorods (AuNRs)-based nanocomposite of CRISPR-Cas9 mild-photothermal via tumor-targeted gene editing. AuNRs are modified azobenzene-4,4'-dicarboxylic acid (p-AZO) achieve...
EGFR tyrosine kinase inhibitors (EGFR-TKIs) have garnered substantial clinical success in treating nonsmall cell lung cancer (NSCLC) harboring epidermal growth factor receptor (EGFR) mutations. However, the inevitable emergence of drug resistance, frequently attributed to activation, mutation, or deletion multiple signaling pathways, poses a significant challenge. Notably, loss PTEN protein expression has emerged as pivotal mechanism fostering resistance mutant cancers. Consequently,...
Abstract BACKGROUND. Invasive aspergillosis (IA) is a leading cause of death in patients with leukemia and those who have undergone hematopoietic stem cell transplantation. Laboratory studies demonstrated that iron essential for Aspergillus growth virulence. METHODS. In the current study, authors retrospectively evaluated bone marrow stores (BMIS) as well recipients allogeneic transplantation IA (n = 33) without fungal infections 33). The first available biopsy specimens prior to diagnosis...
Methods that can detect and quantify single nucleotide variations (SNVs)/single polymorphisms (SNPs) are greatly needed in the bioanalytical measurement of gene mutations polymorphisms. Herein a visual instrument-free SNV quantification platform is developed. Platinum nanoparticles tethered to magnetic beads by single-stranded DNAs designed as quantitative readout reporters for CRISPR-Cas12a nucleic acid detection system. The integration platinum nanoreporter CRISPR-Cas system with...
PTEN [phosphatidylinositol (3,4,5)-trisphosphate phosphatase and tensin homolog deleted from chromosome 10], a critical tumor suppressor, is regulated by numerous post-translational modifications, including phosphorylation, ubiquitination, acetylation, SUMOylation, which affect localization protein stability. Here we report ADP-ribosylation as new modification of PTEN. We identified novel substrate tankyrases, are members the poly(ADP-ribose) polymerases (PARPs). showed that tankyrases...
Manipulation of CRISPR delivery for stimuli-responsive gene editing is crucial cancer therapeutics through maximizing efficacy and minimizing side-effects. However, realizing controlled synergistic combination therapy remains a key challenge. Here, near-infrared (NIR) light-triggered thermo-responsive copper sulfide (CuS) multifunctional nanotherapeutic platform constructed to achieve release CRISPR-Cas9 ribonucleoprotein (RNP) doxorubicin tumor involving in therapy, mild-photothermal (PTT),...
Pesticide residues pose a significant threat to food safety and human health, necessitating the development of novel detection tools. Pesticides can inhibit activity certain biological enzymes, so enzyme inhibition is one methods pesticide detection. In this study, we developed near-infrared fluorescent probe named
Abstract Purpose: Diffuse large B-cell lymphoma (DLBCL) is molecularly and clinically heterogeneous, can be subtyped according to genetic alterations, cell-of-origin, or microenvironmental signatures using high-throughput genomic data at the DNA RNA level. Although proteomic profiling has not been available for DLBCL subtyping, MYC/BCL2 protein double expression (DE) an established prognostic biomarker in DLBCL. The purpose of this study reveal relative roles genetic, phenotypic, biomarkers....
Telomere maintenance is essential for organisms with linear chromosomes and carried out by telomerase during cell cycle. The precise mechanism which cycle controls telomeric access of telomere elongation in mammals remains largely unknown. Previous work has established oligonucleotide/oligosaccharide binding (OB) fold-containing protein TPP1, formerly known as TINT1, PTOP, PIP1, a key factor that regulates recruitment activity. However, the role TPP1 cycle-dependent unclear. Here, we report...
Abstract The extensive phenotypic and functional heterogeneity of cancer cells plays an important role in tumor progression therapeutic resistance. Characterizing this identifying invasive phenotype may provide possibility to improve chemotherapy treatment. By mimicking cell perfusion through circulatory system metastasis, we develop a unique microfluidic cytometry (MC) platform separate at high throughput further derive physical parameter ‘transportability’ characterize the ability pass...
Successful single-cell isolation is a primary step for subsequent chemical and biological analyses of single cells. Conventional methods often encounter operational complexity, limited efficiency, deterioration cell viability, incompetence in the into nanoliter liquid, and/or inability to select adherent cells with specific phenotypes. Here, we develop hand-held pipet (hSCP) that rapid, operationally simple, highly efficient, inexpensive unbiased viable suspended directly from submicroliter...