A5079443631- Autophagy in Disease and Therapy
- Cytomegalovirus and herpesvirus research
- Viral-associated cancers and disorders
- Herpesvirus Infections and Treatments
- Toxoplasma gondii Research Studies
- Viral gastroenteritis research and epidemiology
- Mosquito-borne diseases and control
- Virus-based gene therapy research
- Animal Virus Infections Studies
- RNA Interference and Gene Delivery
- interferon and immune responses
- Viral Infections and Immunology Research
- Phagocytosis and Immune Regulation
- Cytokine Signaling Pathways and Interactions
- Vector-borne infectious diseases
- Immune Cell Function and Interaction
- Inflammasome and immune disorders
- SARS-CoV-2 and COVID-19 Research
- Calcium signaling and nucleotide metabolism
- Epigenetics and DNA Methylation
- Bacillus and Francisella bacterial research
- Monoclonal and Polyclonal Antibodies Research
- Extracellular vesicles in disease
- Plant responses to water stress
- RNA modifications and cancer
VIR Biotechnology (United States)
2020-2025
Broad Institute
2025
Vir Biotechnology (Switzerland)
2022-2025
University of Chicago
2014-2024
University of San Francisco
2024
University of California, San Francisco
2022
Kyungpook National University
2022
Washington University in St. Louis
2012-2013
University of California, Los Angeles
2005-2012
University of Missouri–St. Louis
2012
Melanoma is one of the most deadly and therapy-resistant cancers. Here we show that N
ABSTRACT Sotrovimab (VIR-7831) and VIR-7832 are dual action monoclonal antibodies (mAbs) targeting the spike glycoprotein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). were derived from a parent antibody (S309) isolated memory B cells 2003 (SARS-CoV) survivor. Both mAbs contain an “LS” mutation in Fc region to prolong serum half-life. In addition, encodes GAALIE that has been shown previously evoke CD8+ T-cells context vivo viral infection. neutralize wild-type variant...
The selective autophagy substrate p62 serves as a molecular link between and cancer. Suppression of causes accumulation thereby contributes to tumorigenesis. Here we demonstrate that deficiency promotes cell proliferation migration through p62-dependent stabilization the oncogenic transcription factor Twist1. binds Twist1 inhibits degradation In mice, up-regulation tumor growth metastasis in Twist1-dependent manner. Our findings is key downstream effector regulation suggest targeting...
ABSTRACT Kaposi's sarcoma (KS)-associated herpesvirus (KSHV) open reading frame 50 (ORF50) encodes a viral transcriptional activator, which binds to the KSHV promoter and stimulates transcription of early late genes, thus activating lytic cycle KSHV. We report here that ORF50 cellular proteins CREB-binding protein (CBP) histone deacetylase (HDAC) these binding events modulate ORF50-activated transcription. Binding CBP HDAC activates represses, respectively, ORF50-mediated was shown bind C/H3...
With the advent of complete genome sequences, large-scale functional analyses are generating new excitement in biology and medicine. To facilitate genomewide analyses, we developed a high-density cell array with quantitative automated readout fitness. Able to print at >×10 higher density on standard microtiter plate area than currently possible, our allows single-plate screening set Saccharomyces cerevisiae gene-deletion library significantly reduces amount small molecules other materials...
γ-Herpesviruses, Epstein–Barr virus, and Kaposi's sarcoma-associated herpesvirus are important human pathogens, because they involved in tumor development. Murine γ-herpesvirus-68 (MHV-68 or γHV-68) has emerged as a small animal model system for the study of γ-herpesvirus pathogenesis host–virus interactions. To identify genes required viral replication vitro vivo , we generated 1,152 mutants using signature-tagged transposon mutagenesis on an infectious bacterial artificial chromosome...
Abstract Murine norovirus (MNV) is a positive‐sense, plus‐stranded RNA virus in the Caliciviridae family. It most common pathogen biomedical research colonies. MNV also related to human noroviruses, which cause majority of nonbacterial gastroenteritis worldwide. Like an enteric that replicates intestine and transmitted by fecal‐oral route. murine macrophages dendritic cells culture host. This often used study mechanisms biology, because noroviruses are refractory growth cell culture....
LC3 has been used as a marker to locate autophagosomes. However, it is also well established that can localize on various membranous structures other than We recently demonstrated the conjugation system (ATG7, ATG3, and ATG12–ATG5-ATG16L1) required target IFNG (interferon, gamma)-inducible GTPases parasitophorus vacuole membrane (PVM) of protist parasite Toxoplasma gondii consequently for control T. infection. Here we show not only LC3, but its homologs (GABARAP, GABARAPL1, GABARAPL2) PVM in...
A multifunctional transcription co-activator, cAMP response element-binding protein-binding protein (CBP)interacts with a number of cellular factors and participates in cell growth, transformation, development. It is also targeted by many viral proteins for their transcriptional activity or the regulation processes. Here, we report that C/H3 region CBP latency associated nuclear antigen (LANA) Kaposi's sarcoma-associated herpesvirus (KSHV). LANA interferes interaction between c-Fos,...
Gammaherpesviruses establish life-long persistency inside the host and cause various diseases during their persistent infection. However, systemic interaction between virus in vivo has not been studied individual hosts continuously, although such information can be crucial to control infection of gammaherpesviruses. For noninvasive continuous monitoring gammaherpesvirus host, a recombinant murine 68 (MHV-68, 68) was constructed express firefly luciferase gene driven by viral M3 promoter...
γ-herpesviruses (γHVs) have developed an interaction with their hosts wherein they establish a life-long persistent infection and are associated the onset of various malignancies. One critical virulence factor involved in persistency murine γ-herpesvirus 68 (γHV68) is viral homolog Bcl-2 protein (vBcl-2), which has been implicated to counteract both host apoptotic responses autophagy pathway. However, relative significance two activities vBcl-2 yet be elucidated. Here, by characterizing...
Cytosolic bacterial pathogens must evade intracellular innate immune recognition and clearance systems such as autophagy to ensure their survival proliferation. The cycle of the bacterium Francisella tularensis is characterized by rapid phagosomal escape followed extensive proliferation in macrophage cytoplasm. replication, but not escape, requires locus FTT0369c, which encodes dipA gene (deficient replication A). Here, we show that a replication-deficient, ∆dipA mutant prototypical SchuS4...
Autophagy is a key innate immune response to intracellular parasites that promotes their delivery degradative lysosomes following detection in the cytosol or within damaged vacuoles. Like Listeria and Shigella, which use specific mechanisms avoid autophagic capture, bacterial pathogen Francisella tularensis proliferates of macrophages without demonstrable control by autophagy. To examine how evades autophagy, we screened library F. subsp. Schu S4 HimarFT transposon mutants GFP-LC3-expressing...