Silvia Meschi
A5046686203- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Viral Infections and Outbreaks Research
- Animal Virus Infections Studies
- SARS-CoV-2 detection and testing
- Poxvirus research and outbreaks
- Bacillus and Francisella bacterial research
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- Herpesvirus Infections and Treatments
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- Influenza Virus Research Studies
- Hepatitis B Virus Studies
- Malaria Research and Control
- Long-Term Effects of COVID-19
- Hepatitis C virus research
- Multiple Sclerosis Research Studies
- vaccines and immunoinformatics approaches
- COVID-19 epidemiological studies
- Hepatitis Viruses Studies and Epidemiology
- Disaster Response and Management
Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani
2015-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2010-2025
State Key Laboratory of Virology
2021
Office of Infectious Diseases
2021
Translational Research Institute
2021
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes
2021
Center for Prevention and Treatment of Infections
2021
Aix-Marseille Université
2020
Inserm
2020
Méditerranée Infection Foundation
2020
Analysis of 179 new Ebola virus sequences from patient samples collected in Guinea between March 2014 and January 2015 shows how different lineages evolved spread West Africa. Miles Carroll colleagues report describe the genetic evolution circulating Africa, based on 2015. Their analysis Africa Sierra Leone, Liberia. is currently witnessing most extensive (EBOV) outbreak so far recorded1,2,3. Until now, there have been 27,013 reported cases 11,134 deaths. The origin thought to a zoonotic...
Data on SARS-CoV-2 vaccine immunogenicity in PLWH are currently limited. Aim of the study was to investigate according current CD4 T-cell count.
The pathogenesis of SARS-CoV-2 remains to be completely understood, and detailed cellular cytopathic effects requires definition. We performed a comparative ultrastructural study SARS-CoV-1 infection in Vero E6 cells lungs from deceased COVID-19 patients. induces rapid death associated with profound changes cells. Type II pneumocytes lung tissue showed prominent altered features numerous vacuoles swollen mitochondria presence abundant lipid droplets. accumulation lipids was the most striking...
In order to investigate safety and immunogenicity of SARS-CoV-2 vaccine third dose in people living with HIV (PLWH), we analyze anti-RBD, microneutralization assay IFN-γ production 216 PLWH on ART advanced disease (CD4 count <200 cell/mm
Objective To assess in rheumatoid arthritis (RA) patients, treated with different immunosuppressive therapies, the induction of SARS-CoV-2-specific immune response after vaccination terms anti-region-binding-domain (RBD)-antibody- and T-cell-specific responses against spike, vaccine safety clinical impact on disease activity. Methods Health care workers (HCWs) RA having completed BNT162b2-mRNA last 2 weeks, were enrolled. Serological was evaluated by quantifying anti-RBD antibodies, while...
Specific memory B cells and antibodies are a reliable read-out of vaccine efficacy. We analysed these biomarkers after one two doses BNT162b2 vaccine. The second dose significantly increases the level highly specific antibodies. Two months dose, antibody levels decline, but continue to increase, thus predicting sustained protection from COVID-19. show that although mucosal IgA is not induced by vaccination, migrate in response inflammation secrete at sites. first may lead an insufficient...
SARS-CoV-2 serum neutralization assay represents the gold standard for assessing antibody-mediated protection in naturally infected and vaccinated individuals. In present study, 662 samples collected from February 2020 to January 2021 acute convalescent COVID-19 patients were tested determine neutralizing antibody (NAb) titers using a microneutralization test (MNT) live SARS-CoV-2. Moreover, anti-SARS-CoV-2 IgG, IgA, IgM directed against different viral antigens measured by high-throughput...
Vaccination is the main public health measure to reduce SARS-CoV-2 transmission and hospitalization, a massive worldwide scientific effort resulted in rapid development of effective vaccines. This work aimed define dynamics humoral cell-mediated immune response cohort care workers (HCWs) who received two-dose BNT162b2-mRNA vaccination. The serological was evaluated by quantifying anti-RBD neutralizing antibodies. performed whole blood test Th1 cytokines (IFN-γ, TNF-α, IL-2), produced spike...
Abstract Objectives Simple and standardized methods to establish correlates vaccine-elicited SARS-CoV-2 protection are needed. Methods An observational study on antibody response a mRNA vaccine (Comirnaty) was performed health care workers (V, n=120). Recovered COVID-19 patients (N, n=94) were used for comparison. Antibody evaluated by quantitative anti-receptor binding domain IgG (anti-RBD) commercial assay virus microneutralization test (MNT), in order threshold of anti-RBD units (BAU)...
Objective To assess the kinetics of humoral and cell-mediated responses after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccination in rheumatoid arthritis (RA) patients treated with different immunosuppressive therapies. Methods Following vaccine completed schedule, health care workers (HCWs, n = 49) RA (n 35) were enrolled at 5 weeks (T1) 6 months (T6) first dose BNT162b2-mRNA vaccination. Serological response was assessed by quantifying anti-receptor-binding domain...
We report the follow-up laboratory investigation of three MPXV cases infected in May-June 2022 from diagnosis to disease resolution, monitoring viral shedding different body fluids and antibody kinetics. Out 138 non-lesion samples, DNA was found 92.3% saliva, 85.7% semen, 86.2% oropharyngeal swabs, 51.7% plasma, 46.1% stool, 9.5% urine samples. Viral load quantified by digital PCR widely varied, but tend be higher stool. Replication competent virus recovered four out seventeen including 1...
The coronavirus disease 2019 (COVID-19) pandemic caused by the emergent severe acute respiratory syndrome 2 (SARS-CoV-2) threatens global public health, and there is an urgent need to develop safe effective vaccines. Here, we report generation preclinical evaluation of a novel replication-defective gorilla adenovirus-vectored vaccine encoding pre-fusion stabilized Spike (S) protein SARS-CoV-2. We show that our candidate, GRAd-COV2, highly immunogenic both in mice macaques, eliciting...
Limited data are available on risks and benefits of anti-SARS-CoV2 vaccination in solid organ transplant recipients, weaker responses have been described. At the Italian National Institute for Infectious Diseases, 61 liver recipients underwent testing to describe dynamics humoral cell-mediated immune response after two doses mRNA vaccines compared with 51 healthy controls. Humoral was measured by quantifying both anti-spike neutralizing antibodies; PBMC proliferation assay IFN-γ IL-2...
Vaccine is the main public health measure to reduce SARS-CoV-2 transmission and hospitalization, a massive scientific effort worldwide resulted in rapid development of effective vaccines. This work aimed define dynamics persistence humoral cell-mediated immune response Health Care Workers who received two-dose BNT162b2-mRNA vaccination. Serological was evaluated by quantifying anti-RBD neutralizing antibodies while performed whole blood test Th1 cytokines (IFN-γ, TNF-α, IL-2) produced Spike...
ObjectivesTo characterize the kinetics of humoral and T-cell responses in rheumatoid arthritis (RA)-patients followed up to 4-6 weeks (T3) after SARS-CoV-2 vaccine booster dose.MethodsHealth care workers (HCWs, n = 38) patients with RA (n 52) completing messenger RNA vaccination schedule were enrolled at T3. In each cohort, 25 subjects sampled 5 (T1) 6 months (T2) from first dose. The response was assessed by measuring anti-receptor-binding domain (RBD) neutralizing antibodies,...
ObjectivesWe assessed vaccination-induced antibody and cellular responses against spike from the ancestral strain delta (δ) SARS-CoV-2 variant in patients with immune-mediated inflammatory diseases (IMIDs) on immunosuppressive therapy comparison immunocompetent subjects.MethodsWe enrolled IMID subjects who completed vaccination schedule within 4–6 months first dose. The interferon (IFN)-γ-response to peptides that were derived δ measured by ELISA. Anti-Receptor Binding Domain IgG antibodies...
The new Omicron variant of SARS-CoV-2, first identified in November 2021, is rapidly spreading all around the world. has become dominant SARS-CoV-2. There are many ongoing studies evaluating effectiveness existing vaccines. Studies on neutralizing activity vaccinated sera against currently being carried out laboratories. In this study, we have shown SARS-CoV-2 compared to reference Wuhan D614G individuals with two doses Sputnik V up 6 months after vaccination and who experienced infection...