A5107500226- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Biosensors and Analytical Detection
- HIV Research and Treatment
- Respiratory viral infections research
- HIV/AIDS drug development and treatment
- COVID-19 diagnosis using AI
- Viral gastroenteritis research and epidemiology
- Dental Research and COVID-19
- HIV/AIDS Research and Interventions
- Long-Term Effects of COVID-19
- HIV-related health complications and treatments
- Pneumonia and Respiratory Infections
- Advanced biosensing and bioanalysis techniques
- Influenza Virus Research Studies
- Poxvirus research and outbreaks
- RNA Research and Splicing
- Mosquito-borne diseases and control
- Bacillus and Francisella bacterial research
- Herpesvirus Infections and Treatments
- Extracellular vesicles in disease
- Bee Products Chemical Analysis
- Retinal and Optic Conditions
- Cardiovascular Effects of Exercise
Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani
2020-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2020-2024
Istituto Superiore di Sanità
2023
To investigate the dynamic association among binding and functional antibodies in health-care-workers receiving two doses of BNT162b2 mRNA COVID-19-vaccine, SARS-CoV-2 anti-RBD IgG, anti-Trimeric-S neutralizing (Nabs) were measured serum samples collected at 2 weeks, 3 months, 6 months from full vaccination. Despite high correlation, results for anti-Trimeric S IgG numerically different even after recalculation to BAU/mL following WHO standards indications. Moreover, a peak response levels...
Mpox, caused by the Monkeypox virus (MPV), is a global public health threat. Virus isolation gold standard to confirm MPV infection, but this process can face many challenges. As an alternative, new method was developed in vitro settings using 50 µM of propidium monoazide (PMAxx, DNA-binding agent) coupled with digital droplet PCR (ddPCR). Frozen clinical samples analyzed PMAxx-ddPCR had median 0.8 copies/µL, while untreated 29.8 copies/µL. Since substantial percentage reduction observed...
The mpox virus (MPXV) Clade IIb outbreak in 2022 was the biggest one ever to occur outside Africa, causing different types of clinical symptoms and levels disease severity. There is no currently approved treatment for mpox, but Tecovirimat has proven effective against known orthopoxviruses several animal models Vero cell cultures. Since serious complications, including lung involvement, have been reported, especially immunocompromised people, we investigated effects MPXV infection on vitro...
The global outbreak of severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) and the strategies adopted by different nations have affected altered transmission pathogens around world. We examined impact SARS-CoV-2 on spread viruses in period between 2021 2024 patients with influenza-like symptoms Lazio region using multiplex PCR tests for identification common seasonal viruses. Our data reveal a change from to 2024, sharp decline rise other viruses, especially influenza human...
Although evidence exists on the potential involvement of circular RNAs (circRNAs) in pathogenesis several viral infections, expression levels, and exact role that hsa_circ_0006459 hsa_circ_0015962 could play during Dengue virus (DENV) infection are still unclear. These two circRNAs were identified as possible biomarkers for diagnosis prognosis DENV disease peripheral blood mononuclear cells (PBMC) Dengue-positive patients. This study aimed to evaluate levels DENV-infected patients compare...
ABSTRACT Dengue, a mosquito‐borne disease caused by the dengue virus (DENV), is constantly expanding worldwide. We investigated presence and persistence of DENV RNA in bloodstream other body fluids to describe viral kinetics human host. longitudinally collected serum ( n = 118), plasma 110), whole blood 90), urine oral swabs 68), saliva 42), semen 23), vaginal 49) from 42 patients. measured for median 28 (range 1–63) days symptom onset (DSO). estimated probability detection applying...
Abstract Objectives Compared to RT-PCR, lower performance of antigen detection assays, including the Lumipulse G SARS-CoV-2 Ag assay, may depend on specific testing scenarios. Methods We tested 594 nasopharyngeal swab samples from individuals with COVID-19 (RT-PCR cycle threshold [Ct] values ≤ 40) or non-COVID-19 (Ct >40) diagnoses. RT-PCR positive were assigned diagnostic, screening, monitoring groups testing. Results With a limit 1.2 × 10 4 RNA copies/mL, showed percent agreement (PPA)...
Abstract Since August 2023, outbreaks of dengue virus (DENV) infection have occurred in Italy. We report 2 autochthonous case-patients and their extended follow-up. Despite persistent DENV detected blood by PCR, results for antigenomic RNA were negative after day 5, suggesting that a 5-day isolation period is adequate to avoid secondary cases.
Patients with severe respiratory syndrome caused by SARS-CoV-2 undergo cardiac complications due to hyper-inflammatory conditions. Although the presence of virus has been detected in myocardium infected patients, and infection induced pluripotent cell-derived cardiomyocytes demonstrated, reported expression Angiotensin-Converting Enzyme-2 (ACE2) stromal cells suggests that may determine injury sustaining productive increasing inflammation.We analysed ACE2 receptor primary human derived from...
Diagnostic methods based on SARS-CoV-2 antigens detection are a promising alternative to RNA amplification. We evaluated the automated chemiluminescence-based Lumipulse® G Ag assay saliva samples, using Simplexa™ COVID-19 Direct as reference test. Analytical performance was established pool of healthy donors’ samples spiked with 2019-nCoV/Italy-INMI1 isolate, whereas clinical assessed fresh specimens collected from hospitalized patients suspect or confirmed diagnosis. The limit (LOD) 0.65...
Except for specific vaccines and monoclonal antibodies, effective prophylactic or post-exposure therapeutic treatments are currently limited COVID-19. Propolis, a honeybee's product, has been suggested as potential candidate treatment of COVID-19 its immunomodulatory properties powerful activity against various types viruses, including common coronaviruses. However, direct evidence regarding the antiviral activities this product still remains poorly documented. VERO E6 CALU3 cell lines were...
The pathogenesis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection remains unclear. We report the detection viral RNA from different anatomical districts and antibody profile in first COVID-19 cases diagnosed Italy. tested for SARS-CoV-2 clinical samples, either nonrespiratory (ie, saliva, serum, urine, vomit, rectal, ocular, cutaneous, cervico-vaginal swabs), longitudinally collected both patients throughout hospitalization. Serological analysis was carried out on...
RT-PCR on nasopharyngeal (NPS)/oropharyngeal swabs is the gold standard for diagnosis of SARS-CoV-2 infection and viral load monitoring. Oral fluid (OF) an alternate clinical sample, easy safer to collect could be useful COVID-19 diagnosis, monitoring shedding.Optimal assay conditions analytical sensitivity were established commercial Simplexa™ Direct adapted OF matrix. The was used test 337 NPS specimens collected in parallel from 164 hospitalized patients; 50 bronchoalveolar lavage (BAL) a...
To compare the detection of SARS-CoV-2 Omicron variant in nasopharyngeal-swab (NPS) and oral saliva samples. 255 samples were obtained from 85 Omicron-infected patients. load was measured NPS by using Simplexa™ COVID-19 direct Alinity m AMP assays. Results with two diagnostic platforms showed very good inter-assay concordance (91.4 82.4% for samples, respectively) a significant correlation among cycle threshold (Ct) values. Both revealed highly Ct matrices. Although median value lower than...
A descriptive analysis of common respiratory pathogens (CRPs) detected in nasopharyngeal swabs (NPSs) from hospitalized patients with influenza-like illness during the fall seasons past three years, 2019-2021, Lazio region, Italy, was conducted to assess whether or not CRP circulation changed because COVID-19 season. The results observed a total 633 NPSs subjected molecular diagnosis for CRPs by multiplex PCR assay autumn (exactly week 41 50) were compared each other. In 2019, 144 NPSs, more...
SARS-CoV-2 is a highly infectious virus responsible for the COVID-19 pandemic. Therefore, it important to assess risk of infection, especially in persistently positive patients. Rapid discrimination between and non-infectious viruses aids determining whether prevention, control, treatment measures are necessary. For this purpose, method was developed utilized involving pre-treatment with 50 µM propidium monoazide (PMAxx, DNA intercalant) combined digital droplet PCR (ddPCR). The ddPCR...
The 2022 global mpox outbreak was driven by human-to-human transmission, but modes of transmission sexual relationship versus contact remain unclear. We evaluated using monkeypox virus (MPXV) G2R-mRNA as a marker ongoing viral replication through in vitro experiments. analyzed clinical samples 15 MPXV-positive patients Italy from different biological regions the setup method. presence MPXV DNA, G2R-mRNA, or both all lesion swab samples, independent load, confirmed higher infectivity risk...
New methods of HIV-1 RNA quantification based on dual-target detection are increasingly used in HIV viral load monitoring, but clinical implications and impact infection management not established. Aptima Quant Dx assay is a last generation method, that uses pol LTR as simultaneous target, providing quantitative results mainly while target to report the when signal absent. In our laboratory, about 6% all tests performed with this platform one year period resulted from signal. Interestingly,...