A5054248166- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Viral Infections and Vectors
- Plant Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Mosquito-borne diseases and control
- COVID-19 Clinical Research Studies
- vaccines and immunoinformatics approaches
- Viral Infections and Immunology Research
- HIV Research and Treatment
- Herpesvirus Infections and Treatments
- Poxvirus research and outbreaks
- Animal Virus Infections Studies
- Viral Infections and Outbreaks Research
- HIV/AIDS drug development and treatment
- COVID-19 epidemiological studies
- Virus-based gene therapy research
- Hepatitis C virus research
- Monoclonal and Polyclonal Antibodies Research
- Mycobacterium research and diagnosis
- Vector-Borne Animal Diseases
- HIV/AIDS Research and Interventions
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani
2015-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2019-2024
National Institute of Infectious Diseases
2017
Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana
2017
Università degli Studi della Tuscia
2013
Since May 2022, an outbreak of monkeypox has been ongoing in non-endemic countries. We report four cases Italy young adult men reporting condomless sexual intercourse. The patients are good clinical condition with no need for specific antiviral drugs. Biological samples from seminal fluid were positive viral DNA. For many other viruses found semen there is evidence transmission. possibility transmission virus needs to be investigated.
Since December 2019, SARS-CoV-2 infection has been still rapidly spreading, resulting in a pandemic, followed by an increasing number of cases countries throughout the world. The severity disease depends on patient's overall medical condition but no appropriate markers are available to establish prognosis patients. We performed 16S rRNA gene sequencing, revealing altered composition nasal/oropharyngeal (NOP) microbiota 21 patients affected COVID-19, paucisymptomatic or Intensive Care Unit...
COronaVIrus Disease-2019 (COVID-19) is a pandemic respiratory infection caused by new betacoronavirus, the Severe Acute Respiratory Syndrome-CoronaVirus-2 (SARS-CoV-2). Few data are reported on gut microbiota in COVID-19 patients. 16S rRNA gene sequencing was performed to reveal an altered composition of patients with pneumonia admitted intensive care unit (ICU) (i-COVID19), or infectious disease wards (w-COVID19) as compared controls (CTRL). i-COVID19 showed decrease Chao1 index CTRL and...
Between August and September 2023, three distinct autochthonous dengue virus transmission events occurred in Lazio, Italy, with the main event Rome. The involved different serotypes. No link previous imported cases was identified. Here we describe epidemiological phylogenetic analysis of first implemented control actions. multiple call for a strengthening vector strategies future research to better characterise risk countries like Italy.
We report whole-genome and intra-host variability of SARS-Cov-2 assessed by next generation sequencing (NGS) in upper (URT) lower respiratory tract (LRT) from COVID-19 patients. The aim was to identify possible tissue-specific patterns signatures variant selection for each compartment. Six patients, admitted the Intensive Care Unit, were included study. Thirteen URT LRT analyzed NGS amplicon-based approach on Ion Torrent Platform. Bioinformatic analysis performed using both realized in-house...
Abstract We report phylogenetic and mutational analysis of severe acute respiratory syndrome coronavirus 2 virus strains from the Lazio region Italy provide information about dynamics spread. Data suggest effective containment clade V strains, but subsequently, multiple waves G were circulating widely in Europe.
With numbers of COVID-19 cases having substantially increased at the end 2022 in China, some countries have started or expanded testing and genomic surveillance travellers. We report screening results Italy late December 556 flight passengers provenance from two Chinese provinces. Among these passengers, 126 (22.7%) tested SARS-CoV-2 positive. Whole genome sequencing 61 passengers’ positive samples revealed Omicron variants, notably sub-lineages BA.5.2.48, BF.7.14 BQ.1.1, line with data...
Abstract Whole‐genome sequencing (WGS) has been widely used for the genomic characterization and phylogenesis of mpox virus (MPXV) 2022 multi‐country outbreak. To date, no evidence reported on intra‐host evolution within samples collected over time from a single patient with long‐term infection. Fifty‐one were five patients at different points post‐symptom onset. All confirmed as MPXV DNA positive, amplified by multiplexed PCR amplicon, sequenced WGS. Complete genomes assembled reference...
Our objectives for this study were to understand the biological basis of meat tenderness and provide an overview gene expression profiles related quality as a tool selection. Through deep mRNA sequencing, we analyzed in muscle tissues two Italian cattle breeds: Maremmana Chianina. We uncovered several differentially expressed genes that encode proteins belonging family tripartite motif proteins, which are involved growth, cell differentiation apoptosis, such TRIM45, or play essential role...
The continuous emergence of SARS-CoV-2 variants and subvariants poses significant public health challenges. latest designated subvariant JN.1, with all its descendants, shows more than 30 mutations in the spike gene. JN.1 has raised concerns due to genomic diversity potential enhance transmissibility immune evasion. This study aims analyse molecular characteristics JN.1-related lineages (JN.1*) identified Italy from October 2023 April 2024 evaluate neutralization activity against a subsample...
In January 2015, during a 3-week period, 12 captive Tonkean macacques at sanctuary in Italy died. An orthopoxvirus infection was suspected because of negative-staining electron microscopy results. The diagnosis confirmed by histology, virus isolation, and molecular analysis performed on different organs from all animals. epidemiologic investigation unable to define the source surrounding area. Trapped rodents were negative virologic testing, but specific IgG detected 27.27% small 14.28%...
The Republic of Congo (RoC) declared a chikungunya (CHIK) outbreak on 9 February 2019. We conducted ONE-Human-Animal HEALTH epidemiological, virological and entomological investigation. Methods: collected national surveillance epidemiological data. CHIK diagnosis was based RT-PCR CHIKV-specific antibodies. Full CHIKV genome sequences were obtained by Sanger MinION approaches Bayesian tree phylogenetic analysis performed. Mosquito larvae 215 adult mosquitoes in different villages Kouilou...
Abstract Despite the higher transmissibility of Omicron Variant Concern (VOC), several reports have suggested lower risk for hospitalization and severe outcomes compared to previous variants SARS‐CoV‐2. This study, enrolling all COVID‐19 adults admitted a reference hospital who underwent both S‐gene‐target‐failure test VOC identification by Sanger sequencing, aimed describe evolving prevalence Delta compare main in‐hospital severity, during trimester (December 2021 March 2022) VOCs'...
Abstract Molnupiravir, an oral direct‐acting antiviral effective in vitro against SARS‐CoV‐2, has been largely employed during the COVID‐19 pandemic, since December 2021. After marketing and widespread usage, a progressive increase SARS‐CoV‐2 lineages characterized by higher transition/transversion ratio, characteristic signature of molnupiravir action, appeared Global Initiative on Sharing All Influenza Data (GISAID) International Nucleotide Sequence Database Collaboration (INSDC)...
Orthopoxviruses (OPVs) are diffused over the complete Eurasian continent, but previously described strains mostly from northern Europe, and few infections have been reported Italy. Here we present extended genomic characterization of OPV Abatino, a novel isolated in Italy an infected Tonkean macaque, with zoonotic potential. Phylogenetic analysis based on 102 conserved genes (core gene set) showed that Abatino is most closely related to Ectromelia virus species (ECTV), although placed...
The pathogenesis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection remains unclear. We report the detection viral RNA from different anatomical districts and antibody profile in first COVID-19 cases diagnosed Italy. tested for SARS-CoV-2 clinical samples, either nonrespiratory (ie, saliva, serum, urine, vomit, rectal, ocular, cutaneous, cervico-vaginal swabs), longitudinally collected both patients throughout hospitalization. Serological analysis was carried out on...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is characterized by fast evolution with the appearance of several variants. Next-Generation Sequencing (NGS) technology considered gold standard for monitoring known and new SARS-CoV-2 However, complexity this renders approach impracticable in laboratories located areas limited resources. We analyzed capability ThermoFisher TaqPath COVID-19 RT-PCR (TaqPath) Seegene Novaplex Variant assay (Novaplex) to detect Omicron variants;...
Abstract A new SARS-CoV-2 clade (GV) characterized by S substitution A222V, first reported from Spain in March, is rapidly spreading across Europe. To establish the A222V variant involvement infection rise Italy, all GISAID sequences Italy and those our Laboratory (Lazio) period June-October were analysed. recognized August, represents 11.2% of this period, reaching 100% autochthonous October, supporting increased GV circulation Italy.
The Crimean–Congo hemorrhagic fever virus (CCHFV) is considered to be a major emerging infectious threat, according the WHO R&D blueprint. A wide range of CCHFV molecular assays have been developed, employing varied primer/probe combinations. high genetic variability often hampers efficacy available tests and can affect their diagnostic potential. Recently, increasing numbers complete genomic sequences become available, allowing better appreciation evolution this virus. We summarized...