A5038511455- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Infective Endocarditis Diagnosis and Management
- COVID-19 Clinical Research Studies
- Reproductive tract infections research
- Genomics and Phylogenetic Studies
- Yersinia bacterium, plague, ectoparasites research
- COVID-19 epidemiological studies
- Sinusitis and nasal conditions
- COVID-19 diagnosis using AI
- Viral gastroenteritis research and epidemiology
- Orthopedic Infections and Treatments
- Viral Infections and Outbreaks Research
- Animal Virus Infections Studies
- Mycobacterium research and diagnosis
- Microbial infections and disease research
- Census and Population Estimation
- Peptidase Inhibition and Analysis
- Immune responses and vaccinations
- Sirtuins and Resveratrol in Medicine
- Plant Virus Research Studies
- Biomarkers in Disease Mechanisms
- Syphilis Diagnosis and Treatment
Istituto Superiore di Sanità
2018-2025
During 2022, Omicron became the dominant SARS-CoV-2 variant in Europe. This study aims to assess impact of such on severe disease from compared with Delta Italy.Using surveillance data, we assessed risk developing COVID-19 infection individuals aged ≥12 years using a multilevel negative binomial model adjusting for sex, age, vaccination status, occupation, previous infection, weekly incidence, and geographical area. We also analyzed interaction between sequenced variant, status.We included...
Background The SARS-CoV-2 variant of concern Omicron was first detected in Italy November 2021. Aim To comprehensively describe spread the 2 subsequent months and its impact on overall circulation at population level. Methods We analyse data from four genomic surveys conducted across country between December 2021 January 2022. Combining sequencing results with epidemiological records collated by National Integrated Surveillance System, reproductive number exponential growth rate are...
In this work, we studied the selective pressure and evolutionary analysis on SARS-CoV-2 BF.7 BQ.1.1 lineages circulating in Italy from July to December 2022. Two different datasets were constructed: first comprised 694 lineage sequences second 734 sequences, available Italian COVID-19 Genomic (I-Co-Gen) platform GISAID (last access date 15 2022). Alignments performed with MAFFT v.7 under Galaxy platform. The HYPHY software was used study pressure. Four positively selected sites (two nsp3 two...
The continuous emergence of SARS-CoV-2 variants and subvariants poses significant public health challenges. latest designated subvariant JN.1, with all its descendants, shows more than 30 mutations in the spike gene. JN.1 has raised concerns due to genomic diversity potential enhance transmissibility immune evasion. This study aims analyse molecular characteristics JN.1-related lineages (JN.1*) identified Italy from October 2023 April 2024 evaluate neutralization activity against a subsample...
The aim of this study was to evaluate the antimicrobial susceptibilities 866 Neisseria meningitidis invasive strains during 11 years surveillance in Italy. Two and six were resistant ciprofloxacin rifampin, respectively. Forty-five percent penicillin intermediate, associated with hypervirulent serogroup C clonal complex 11. All susceptible cephalosporins.
Polyphenols have been widely studied for their antiviral effect against respiratory virus infections. Among these, resveratrol (RV) has demonstrated to inhibit influenza replication and more recently, it tested together with pterostilbene severe acute syndrome coronavirus 2 (SARS-CoV-2) infection. In the present work, we evaluated activity of polydatin, an RV precursor, a mixture polyphenols other micronutrients, named A5+, SARS-CoV-2 To this end, infected Vero E6 cells analyzed both viruses...
Abstract The SARS-CoV-2 variant of concern Omicron was first detected in Italy November 2021. Data from three genomic surveys conducted between December 2021 and January 2022 suggest that became dominant less than one month (prevalence on 3: 78.6%-83.8%) with a doubling time 2.7–3.1 days. mean net reproduction number rose about 1.15 absence to peak 1.83 for symptomatic cases 1.33 hospitalized cases, while it remained stable critical cases.
The aim was to investigate an outbreak of invasive meningococcal disease (IMD) in Southern Sardinia.Epidemiological and microbiological investigations were performed. latter included antimicrobial susceptibility testing whole-genome sequencing (WGS).Seven individuals with severe IMD found be infected serogroup B (MenB) Neisseria meningitidis the first quarter 2018. Five seven cases (five males; mean age 19 years; range 18-21 CFR 40%) due a unique strain B:P1.5-1,10-8:F3-6:ST-11(cc11),...
The four-component meningococcal serogroup B vaccine (4CMenB) contains antigens present in the majority of meningococci causing invasive disease (IMD) and may potentially offer protection against strains belonging to non-B serogroups.This study aimed evaluate ability 4CMenB-induced antibodies kill, a human serum bactericidal assay (hSBA), main genotypes responsible for IMD Italy.Meningococci, collected between 2015 2017, was characterized PorA, FetA sequence type, clonal complex. Twenty...
Neisseria meningitidis is one of the few commensal bacteria that can even cause large epidemics invasive meningococcal disease (IMD). N. meningitis serogroup C belonging to hypervirulent clonal complex 11 (cc11) represents an important public health threat worldwide. We reconstructed dispersal patterns strains C:cc11 by phylogenomic time trees.
Enhanced laboratory-based surveillance of invasive meningococcal disease (IMD) in Italy was only assessed indirectly by numerically comparing data cases with hospital discharge records (HDR). In this study, we evaluated the completeness, timeliness and sensitivity IMD from 2015 to 2018. Completeness were described at national subnational level. A capture-recapture analysis conducted evaluate positive predictive value (PPV) using HDR as external source a combination deterministic...
The emergence of Neisseria gonorrhoeae isolates displaying resistance to antimicrobials, in particular ceftriaxone monotherapy or plus azithromycin, represents a global public health concern. This study aimed analyze the trend antimicrobial 7-year isolate collection retrospective analysis Italy. Molecular typing on subsample gonococci was also included. A total 1,810 culture-positive gonorrhea cases, collected from 2013 2019, were investigated by susceptibility, using gradient diffusion...
The SARS-CoV-2 Delta variant of concern (VOC) was often associated with serious clinical course the COVID-19 disease. Herein, we investigated selective pressure, gene flow and evaluation on frequencies mutations causing amino acid substitutions in three Italian regions. A total 1500 genomes, collected Italy from April to October 2021 were investigated, including a subset 596 pressure frequency prediction their possible impact stability proteins investigated. dataset, this study, identified...
Background In 2015–2016, a cross-sectional carriage survey was performed in Tuscany Region, Italy, during an outbreak of invasive meningococcal disease due to Neisseria meningitidis serogroup C clonal complex 11 (MenC:cc11). This study aims evaluate the genomic profile isolates collected survey. Methods Whole-genome sequencing (WGS) using Illumina MiSeq on 85 cultivated received at Dept. Infectious Disease, National Institute Health (Istituto Superiore di Sanità, ISS), as Reference...
In Italy, serogroup C meningococci of the clonal complex cc11 (MenC/cc11) have caused several outbreaks invasive meningococcal disease (IMD) during past 20 years. Between December 2019 and January 2020, an outbreak six cases IMD infected with MenC/cc11 was identified in a limited area northern part Italy. All presented severe clinical picture, two them were fatal. This report is focused on microbiological molecular analysis isolates aim to reconstruct chain transmission. It further presents...
Background In Italy the introduction of meningococcal C conjugate vaccine in 2005 has led to a significant reduction invasive disease (IMD) caused by Neisseria meningitidis serogroup (MenC). However, this is still responsible sporadic cases, clusters and local outbreaks. The study aims investigate genotype antimicrobial susceptibility profile MenC isolates collected from 2000 2020. Methods Bacterial biological samples (blood or cerebrospinal fluid) cases are characterized at National...
A COVID-19 outbreak occurred at the end of October 2021 among pilgrims returning from Medjugorje (Bosnia and Herzegovina). Whole genome sequencing (WGS) SARS-CoV-2, epidemiological data, phylogenetic analysis were used to reconstruct dynamics. The results suggest that only in one case, associated with SARS-CoV-2 sub-lineage AY.9.2, it is possible trace back place contagion Medjugorje, while other cases likely be acquired country origin. combined use data derived WGS, allowed us study...
The "Investigating and translating genomic evidence for public health response to SARS-CoV-2 (INSIDE SARS-CoV-2)" project is part of the initiative "Joint science technology cooperation call joint proposals years 2021-2023" promoted by Italian Ministry Foreign Affairs International Cooperation (MAECI) Republic India. To start activities, pandemic epidemiological situation in Italy India, together with surveillance strategies virus two countries, are here described.
We investigated the impact of social distancing measures, used to contain or mitigate SARS-CoV-2 spread, on transmission invasive meningococcal disease (IMD) in Italy. To this end, temporal correlation between implementation lockdown measures 2020 and IMD incidence was evaluated. A dramatic decline observed, suggesting that applied Italy affected other infectious diseases transmitted through direct contact droplets, at least early phase COVID-19 pandemic.
Background Neisseria meningitidis (meningococcus) is the causative agent of invasive meningococcal disease (IMD). Meningococcus serogroup B (MenB) one main causing IMD. MenB strains may be prevented by vaccines. In particular, vaccines with Factor H-binding protein (FHbp), classified into two subfamilies (A or B) in three variants (v1, v2 v3), are those available. The objective study was to investigate phylogenetic relationships FHbp A and (variants v1, v3) genes proteins, together their...
Objectives To evaluate and characterise meningococcal carriage among healthy men who have sex with (MSM) within a screening programme for Neisseria gonorrhoeae infection at the San Gallicano Dermatological Institute, Italy. Methods A total of 441 MSM attending STI/HIV Centre Rome, Italy, in 2016 were routinely screened N. by pharyngeal rectal swabs. meningitidis isolates evaluated antibiotic susceptibility characterised whole genome sequencing. Genetic relationships determined using core...