A5072651365- Bacterial Infections and Vaccines
- Reproductive tract infections research
- Pneumonia and Respiratory Infections
- Syphilis Diagnosis and Treatment
- Infective Endocarditis Diagnosis and Management
- Streptococcal Infections and Treatments
- Respiratory viral infections research
- Influenza Virus Research Studies
- Carbohydrate Chemistry and Synthesis
- Virology and Viral Diseases
- Bacterial Identification and Susceptibility Testing
- Polysaccharides and Plant Cell Walls
- COVID-19 Clinical Research Studies
- Sinusitis and nasal conditions
- Microbial Metabolites in Food Biotechnology
- SARS-CoV-2 and COVID-19 Research
- Immune responses and vaccinations
- Enzyme Structure and Function
- Microbial infections and disease research
- bioluminescence and chemiluminescence research
- Antibiotic Use and Resistance
- Legume Nitrogen Fixing Symbiosis
- Pelvic floor disorders treatments
- Plant-Microbe Interactions and Immunity
- SARS-CoV-2 detection and testing
Istituto Superiore di Sanità
2014-2024
University of Naples Federico II
2004-2006
BackgroundGenomic surveillance using quality-assured whole-genome sequencing (WGS) together with epidemiological and antimicrobial resistance (AMR) data is essential to characterise the circulating Neisseria gonorrhoeae lineages their association patient groups (defined by demographic factors). In 2013, European gonococcal population was characterised genomically for first time. We describe in 2018 identify emerging or vanishing associated AMR characteristics of patients, elucidate recent...
BackgroundRegular quality-assured whole-genome sequencing linked to antimicrobial resistance (AMR) and patient metadata is imperative elucidate the shifting gonorrhoea epidemiology, both nationally internationally. We aimed examine gonococcal population in European Economic Area (EEA) 2020, emerging disappearing lineages associated with AMR metadata, compare 2013 2018 data, explain changes epidemiology.MethodsIn this retrospective genomic surveillance study, we analysed consecutive isolates...
A seroprevalence study of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was conducted in a high-incidence area located northeastern Italy.All citizens above 10 years age resident five municipalities the Autonomous Province Trento, with highest incidence disease 2019 (COVID-19) cases, were invited to participate study. Among 6098 participants, 6075 sera and standardized questionnaire administered face-to-face collected between 5 May 15 2020 examined. Symptomatic individuals...
Abstract Objectives Novel antimicrobials for treatment of gonorrhoea are imperative. The first-in-class spiropyrimidinetrione zoliflodacin is promising and currently in an international Phase 3 randomized controlled clinical trial (RCT) uncomplicated gonorrhoea. We evaluated the vitro activity genetic conservation target (GyrB) other potential resistance determinants among 1209 consecutive Neisseria gonorrhoeae isolates obtained from 25 EU/European Economic Area (EEA) countries 2018 compared...
The European Gonococcal Antimicrobial Surveillance Programme (Euro-GASP) performs annual sentinel surveillance of Neisseria gonorrhoeae susceptibility to therapeutically relevant antimicrobials across the Union/European Economic Area (EU/EEA). We present Euro-GASP results from 2019 (26 countries), linked patient epidemiological data, and compared with data previous years.Agar dilution minimum inhibitory concentration (MIC) gradient strip methodologies were used determine antimicrobial (using...
ABSTRACT The meningococcal antigen typing system (MATS) sandwich enzyme-linked immunosorbent assay (ELISA) was designed to measure the immunologic cross-reactivity and quantity of antigens in target strains a pathogen. It first used factor H-binding protein (fHbp), neisserial adhesin A (NadA), heparin-binding (NHBA) content serogroup B (MenB) isolates relative reference strain, or “relative potency” (RP). With PorA genotype, RPs were then assess strain coverage by 4CMenB, multicomponent MenB...
Preliminary results suggest that pertussis infection might be considered in infants during a seasonal respiratory syncytial virus (RSV) outbreak.In order to analyze clinical features and laboratory findings with hospitalized for acute symptoms RSV outbreak, we conducted retrospective single-center study on 19 (6 boys; median age 72 days) matched controls (RSV-bronchiolitis), from October 2008 April 2010. B. were detected nasopharyngeal washes Real Time-PCR.Infants less often breastfeed than...
The sexually transmitted infection gonorrhea remains a public health concern for becoming resistant to drug treatments available. purpose of this study was evaluate the usefulness matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF MS) identify and cluster Neisseria gonorrhoeae. From current monitoring in Italy, as part European Gonococcal Antimicrobial Surveillance Programme (EURO-GASP), 93 gonococci collected from 2007 2012 susceptible (44 isolates) (49...
Onconase (ONC), a member of the RNase A superfamily extracted from oocytes Rana pipiens, is an effective cancer killer. It currently used in treatment various forms cancer. ONC antitumor properties depend on its ribonucleolytic activity that low comparison with other members superfamily. The most damaging side effect renal toxicity, which seems to be caused by unusual stability enzyme. Therefore, mutants reduced thermal and/or increased catalytic may have significant implications for human...
Two clusters of invasive meningococcal disease in the north Italy both due to serogroup C/ST-11 clonal complex are here described. The objective investigation was analyse phenotype and genotype meningococci involved two which were national relevance fatal outcome majority cases (six total 10 cases). All strains C:2a:P1.5 ST-11/ET-37 complex. pulsed field gel electrophoresis (PFGE) variable number tandem repeats (VNTR) profiles identified, one for each cluster. VNTRs different from those...
Confidence in any diagnostic and antimicrobial susceptibility testing data is provided by appropriate regular quality assurance (QA) procedures. In Europe, the European Gonococcal Antimicrobial Susceptibility Programme (Euro-GASP) has been monitoring Neisseria gonorrhoeae since 2004. Euro-GASP includes an external assessment (EQA) scheme as essential component for a quality-assured laboratory-based surveillance programme. Participation EQA enables problems with performed to be identified...
Neisseria gonorrhoeae (NG) antimicrobial susceptibility trends to azithromycin, cefixime and ceftriaxone were analyzed, from 2009 2016, monitor changing concomitant with the change in prescribing practice 2012 cefixime, or ceftriaxone, plus azithromycin. Patient characteristics predictive be infected by antibiotic resistant N. estimated. Finally, protocol for treatment of gonorrhoea, comparison international guidelines, was also evaluated.Data on NG resistance obtained a network sexually...
The emergence of Neisseria gonorrhoeae isolates displaying resistance to antimicrobial agents is a major public health concern and serious issue related the occurrence further untreatable gonorrhea infections. A retrospective analysis on 1,430 N. isolates, collected from 2003 through 2012, for susceptibility by Etest molecular characterization multiantigen sequence typing (NG-MAST) was carried out in Italy. Azithromycin-resistant gonococci decreased 14% 2007 2.2% 2012. Similarly, with high...
Factor H-Binding Protein (fHbp) is a key component of meningococcal vaccines such as MenB-fHbp, licensed in the EU, UK, and other countries. Sufficient expression fHbp on bacterial surface necessary for vaccine-induced antibodies to bind exert bactericidal activity. The flow cytometric MEASURE assay quantifies vitro, previous studies have shown that strains with Mean Fluorescence Intensity (MFI) >1000 are likely be killed by MenB-fHbp-induced antibodies. This study assessed peptide...
Pelvic inflammatory disease (PID), a serious infection in sexually active women, is one of the reasons for which females seek care emergency departments and therefore represents an important public health problem. PID result endocervical with different microorganisms, then ascend to endometrium fallopian tubes. Symptoms may be mild aspecific, making its diagnosis difficult. However, this clinical condition requires effective antibiotic treatment reduce incidence complications late sequelae....
The structure of the complex mixture core oligosaccharide components lipooligosaccharide (LOS) fraction Agrobacterium tumefaciens strain TT111 was determined directly on deacetylated products by means spectroscopical methods. rhamnan elongating inner Kdo residue shares structural features with other polysaccharides from well-known plant pathogenic bacteria. Its conformation through extensive molecular dynamic (MD) analysis and presents an epitope similar to that recognized defense system.
A strictly defined subset of gonococci (n = 65) isolated in Italy from 2011 to 2014 was characterized by antimicrobial susceptibility for cefixime (CFM) and ceftriaxone (CRO) sequencing resistance determinant genes (penA, mtrR, porB1b, ponA) extended-spectrum cephalosporins Neisseria gonorrhoeae multiantigen sequence typing (NG-MAST). The penA mosaic alleles XXXIV XXXV were found all resistant (R) decreased (DS) CFM, except one. They associated with an adenine deletion the mtrR promoter plus...
The emergence of antibiotic resistant strains poses a great concern for gonorrhea treatment. aim this study was to characterize penicillinase-producing Neisseria gonorrhoeae (PPNG) isolates collected in Italy two time frames, 2003-2004 and 2014-2015. A total 80 PPNG were characterized the blaTEM gene variant plasmid type. Furthermore, gonococci typed using multiantigen sequence typing. Antibiotic susceptibility assay performed penicillin, ciprofloxacin, ceftriaxone, spectinomycin by Etest...
In recent decades, Neisseria gonorrhoeae has developed resistance to several antimicrobial classes. Molecular epidemiology approaches are useful for detecting emerging, often resistant, gonococcal clones. this study, 67 N. isolates from different anatomic sites, collected over 8 years in Italy, were analyzed by whole genome sequencing (WGS). WGS was performed using the Illumina NextSeq 500 platform. Phylogenetic analysis based on core single nucleotide polymorphism (SNP) and multilocus...
We report pertussis cases in 4 infants less than 6 months admitted with symptoms compatible to the intensive care unit of Università Cattolica del Sacro Cuore Rome, April 2014. Realtime PCR confirmed diagnosis for infants, 2 them were cousins, and household contacts 1 them. Analysis toxin, its promoter pertactin was also performed. First all, this emphasizes need investigate contact pertussis; secondly, evaluate selective vaccination members newborns as an effective program reduce infants.
Background Typing of Neisseria meningitidis isolates is crucial for the surveillance invasive meningococcal disease (IMD). We performed a molecular epidemiology study N . serogroup B (MenB) causing IMD in Italy between 2014 and 2017 to describe circulating strains belonging this serogroup, with particular regards two factor H-binding protein (FHbp) subfamilies present bivalent MenB vaccine. Materials methods A total 109 culture positive 46 negative samples were collected within National...