A5059452462- Minerals Flotation and Separation Techniques
- Metallurgical Processes and Thermodynamics
- Metal Extraction and Bioleaching
- Yersinia bacterium, plague, ectoparasites research
- Mineral Processing and Grinding
- SARS-CoV-2 and COVID-19 Research
- Reproductive tract infections research
- Bacterial Infections and Vaccines
- Syphilis Diagnosis and Treatment
- Vector-borne infectious diseases
- Urinary Tract Infections Management
- SARS-CoV-2 detection and testing
- Pharmacological Effects of Natural Compounds
- Bacterial Identification and Susceptibility Testing
- Genomics and Phylogenetic Studies
- Extraction and Separation Processes
- COVID-19 Clinical Research Studies
- Flame retardant materials and properties
- Geotechnical and Geomechanical Engineering
- Burkholderia infections and melioidosis
- Clostridium difficile and Clostridium perfringens research
- Zoonotic diseases and public health
- Hematopoietic Stem Cell Transplantation
- Coccidia and coccidiosis research
- Antifungal resistance and susceptibility
University of Helsinki
2002-2022
Helsinki University Hospital
2020-2022
Finnish Red Cross
2013
Background Understanding the false negative rates of SARS-CoV-2 RT-PCR testing is pivotal for management COVID-19 pandemic and it has implications patient management. Our aim was to determine real-life clinical sensitivity RT-PCR. Methods This population-based retrospective study conducted in March–April 2020 Helsinki Capital Region, Finland. Adults who were clinically suspected infection underwent testing, with sufficient data their medical records grading suspicion eligible. In addition...
Many pathogens are equipped with factors providing resistance against the bactericidal action of complement. Yersinia enterocolitica, a Gram-negative enteric pathogen invasive properties, efficiently resists deleterious human The major Y. enterocolitica serum determinants include outer membrane proteins YadA and Ail. Lipopolysaccharide (LPS) O-antigen (O-ag) core (OC) do not contribute directly to complement resistance. aim this study was analyze possible mechanism whereby could inhibit...
Abstract Objectives Novel antimicrobials for treatment of gonorrhoea are imperative. The first-in-class spiropyrimidinetrione zoliflodacin is promising and currently in an international Phase 3 randomized controlled clinical trial (RCT) uncomplicated gonorrhoea. We evaluated the vitro activity genetic conservation target (GyrB) other potential resistance determinants among 1209 consecutive Neisseria gonorrhoeae isolates obtained from 25 EU/European Economic Area (EEA) countries 2018 compared...
The European Gonococcal Antimicrobial Surveillance Programme (Euro-GASP) performs annual sentinel surveillance of Neisseria gonorrhoeae susceptibility to therapeutically relevant antimicrobials across the Union/European Economic Area (EU/EEA). We present Euro-GASP results from 2019 (26 countries), linked patient epidemiological data, and compared with data previous years.Agar dilution minimum inhibitory concentration (MIC) gradient strip methodologies were used determine antimicrobial (using...
Abstract Ail is a 17-kDa chromosomally encoded outer membrane protein that mediates serum resistance (complement resistance) in the pathogenic Yersiniae (Yersinia pestis, Y. enterocolitica, and pseudotuberculosis). In this article, we demonstrate pseudotuberculosis from strains PB1, 2812/79, YPIII/pIB1 (serotypes O:1a, O:1b, O:3, respectively) can bind inhibitor of classical lectin pathways complement, C4b-binding (C4BP). Binding was observed irrespective serotype tested independently YadA,...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused millions of infections and fatalities globally since its emergence in late 2019. The virus was first detected Finland January 2020, after which it rapidly spread among the populace spring. However, compared to other European nations, had a low incidence SARS-CoV-2. To gain insight into origins turnover SARS-CoV-2 lineages circulating we investigated phylogeographic -dynamic history virus.The introductions were inferred...
Abstract Background Accurate molecular assays for prediction of antimicrobial resistance (AMR)/susceptibility in Neisseria gonorrhoeae (Ng) can offer individualized treatment gonorrhoea and enhanced AMR surveillance. Objectives We evaluated the new ResistancePlus® GC assay 23S 2611 (beta) (SpeeDx), resistance/susceptibility to ciprofloxacin azithromycin, respectively. Methods Nine hundred sixty-seven whole-genome-sequenced Ng isolates from 20 European countries, 143 Ng-positive (37 with...
Ten UriSed 3 PRO automated microscopes (77 Elektronika, Hungary) were verified for nine HUSLAB laboratories with 160 000 annual urine samples. Particle counting of the primary instrument was against reference visual microscopy 463 specimens, and culture on chromogenic agar plates parallel 396 specimens. Nine secondary instruments compared pairwise instrument. Relative imprecisions to Poisson distribution, R(CV), estimated be 1.0 white blood cell (WBC) 1.5 red (RBC) counts, respectively....
Abstract The value of surveillance cultures in predicting systemic infections and guiding antimicrobial treatment is controversial. We investigated 57 pediatric allo‐ SCT s between 2007 2009. ALL (34), AML (5), severe aplastic anemia (4) were the largest patient groups. Conditioning was TBI ‐based 87% 54% developed GVHD (21% grade III ‐ IV ). Of 2594 weekly colonization samples, 24% positive (fecal bacteria 86%, fecal fungi 16%, Clostridium difficile 16%; throat 17% 4%). Enterobacteria...
Abstract Importance Understanding the false negative rates of SARS-CoV-2 RT-PCR testing is pivotal for management COVID-19 pandemic and it has practical implications patient in healthcare facilities. Objective To determine real-life clinical sensitivity testing. Design A retrospective study on case series from 4 March – 15 April 2020. Setting population-based conducted primary tertiary care Helsinki Capital Region, Finland. Participants Adults who were clinically suspected infection...
Abstract Finland has had a low incidence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) infections as compared to most European countries. Here we report the origins and turnover SARS-CoV-2 lineages circulating in 2020. introduced January 2020 spread rapidly across southern during spring. We observed rapid among Finnish this period. Clade 20C became prevalent sequenced cases was replaced by other strains fall Bayesian phylogeographic reconstructions suggested 42 independent...
We assessed the possibility to rule out negative urine cultures by counting with UriSed 3 PRO (77 Elektronika, Hungary) at Helsinki and Uusimaa Hospital District. Bacteria of automated microscope was verified reference phase contrast microscopy against growth in culture. After acceptance into routine, results bacteria leukocyte from 56 426 specimens eight instruments were compared parallel samples cultured on chromogenic agar. Laboratory data including preanalytical details accessed through...