A5084050042- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Infectious Diseases and Tuberculosis
- Pneumonia and Respiratory Infections
- Respiratory viral infections research
- Long-Term Effects of COVID-19
- Diagnosis and treatment of tuberculosis
- Influenza Virus Research Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Immune responses and vaccinations
- Viral gastroenteritis research and epidemiology
- Graphene and Nanomaterials Applications
- Immunodeficiency and Autoimmune Disorders
- Antibiotic Resistance in Bacteria
- Head and Neck Cancer Studies
- Kawasaki Disease and Coronary Complications
- COVID-19 Impact on Reproduction
- Bacteriophages and microbial interactions
- Inflammasome and immune disorders
- Immune Response and Inflammation
- SARS-CoV-2 detection and testing
- Sphingolipid Metabolism and Signaling
- Infection Control and Ventilation
Università Cattolica del Sacro Cuore
2016-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2018-2025
Agostino Gemelli University Polyclinic
2012-2025
Corvid Technologies (United States)
2023
University of L'Aquila
2020
Magna Graecia University
2020
Institute of Microbiology
2009-2011
Pontifícia Universidade Católica de São Paulo
2010
Catholic University of America
2010
University of the Sacred Heart
2010
Background: To date, there are no comprehensive data on pediatric COVID-19 from Latin America. This study aims to assess and Multisystem Inflammatory Syndrome (MIS-C) in American children, appropriately plan allocate resources face the pandemic a local international level. Methods: Ambispective multicenter cohort 5 countries. Children 18 years of age or younger with microbiologically confirmed SARS-CoV-2 infection fulfilling MIS-C definition were included. Findings: Four hundred nine...
Although more patients are surviving COVID-19, there emerging data on a substantial proportion of with persisting, and often debilitating, symptoms sequelae for months following acute COVID-19. This scenario is commonly referred to as post-acute SARS-CoV-2 infection (PASC) or long COVID.1Nalbandian A Sehgal K Gupta et al.Post-acute COVID-19 syndrome.Nat Med. 2021; 27: 601-615Crossref PubMed Scopus (2541) Google Scholar Some the most reported fatigue, weakness, breathlessness, chest pain,...
Recent advancements in bidimensional nanoparticles production such as graphene (G) and oxide (GO) have the potential to meet need for highly functional personal protective equipment (PPE) against SARS-CoV-2 infection. The ability of G GO interact with microorganisms provides an opportunity develop engineered textiles use PPE limit spread COVID-19. current high-risk settings COVID transmission only a physical barrier that decreases infection likelihood does not inactivate virus. Here, we show...
The Envelope (E) protein of SARS-CoV-2 is the most enigmatic among four structural ones. Most its current knowledge based on direct comparison to SARS E protein, initially mistakenly undervalued and subsequently proved be a key factor in ER-Golgi localization tight junction disruption. We compared genomic sequences SARS-CoV-2, SARS-CoV closely related genomes bats pangolins obtained from GISAID GenBank databases. When known we observed significant difference amino acid sequence C-terminal...
Interleukin-6 signal blockade showed preliminary beneficial effects in treating inflammatory response against SARS-CoV-2 leading to severe respiratory distress. Herein we describe the outcomes of off-label intravenous use Sarilumab SARS-CoV-2-related pneumonia.53 patients with pneumonia received Sarilumab; pulmonary function improvement or Intensive Care Unit (ICU) admission rate medical wards, live discharge ICU treated and safety profile were recorded. 400 mg was administered intravenously...
We explored the potential link between chronic inflammatory arthritis and COVID-19 pathogenic resolving macrophage pathways their role in pathogenesis. found that bronchoalveolar lavage fluid (BALF) clusters FCN1+ FCN1+SPP1+ predominant severe were transcriptionally related to synovial tissue (STM) CD48hiS100A12+ CD48+SPP1+ drive rheumatoid (RA) synovitis. BALF cluster FABP4+ healthy lung was STM TREM2+ governs resolution of synovitis RA remission. Plasma concentrations SPP1 S100A12 (key...
The long-term outcomes of newborns exposed to SARS-CoV-2 infection in utero or during the first hours life are still unknown. We performed a single-center, prospective, observational study born from mothers with microbiologically confirmed pregnancy at time delivery. Infants were offered multidisciplinary follow-up consisting nasopharyngeal Polymerase Chain Reaction test birth and 48–72 h life, auxological growth neurological development, serologic testing, audiological ophthalmological...
Nutritional status is a critical factor throughout COVID-19 disease course. Malnutrition associated with poor outcomes in hospitalized patients.
Summary The PE family of Mycobacterium tuberculosis includes 98 proteins which share a highly homologous N‐terminus sequence about 110 amino acids (PE domain). Depending on the C‐terminal domain, can be divided in three subfamilies, largest is PE_PGRS with 61 members. In this study, we determined cellular localization by cell fractionation and immunoelectron microscopy expressing chimeric epitope‐tagged recombinant smegmatis . We demonstrate that domain PE_PGRS33 PE11 (a protein constituted...
Extended-spectrum-beta-lactamase (ESBL)-producing strains of Escherichia coli are a significant cause bloodstream infections (BSI) in hospitalized and nonhospitalized patients. We previously showed that delaying effective antimicrobial therapy BSI caused by ESBL producers significantly increases mortality. The aim this retrospective 7-year analysis was to identify risk factors for inadequate initial (IIAT) (i.e., empirical treatment based on drug which the isolate had displayed vitro...
The role and function of PE_PGRS proteins Mycobacterium tuberculosis (Mtb) remains elusive. In this study for the first time, Mtb isogenic mutants missing selected PE_PGRSs were used to investigate their in pathogenesis (TB). We demonstrate that MtbΔPE_PGRS30 mutant was impaired its ability colonize lung tissue cause damage, specifically during chronic steps infection. Inactivation PE_PGRS30 resulted an attenuated phenotype murine human macrophages due inability inhibit phagosome-lysosome...
PE_PGRS represent a large family of proteins typical pathogenic mycobacteria whose members are characterized by an N-terminal PE domain followed Gly-Ala repeat-rich C-terminal domain. Despite the abundance PE_PGRS-coding genes in Mycobacterium tuberculosis (Mtb) genome their role and function biology pathogenesis still remains elusive. In this study, we generated Mtb H37Rv mutant (MtbΔ33) which structural gene PE_PGRS33, prototypical member protein family, was inactivated. We showed that...
Abstract Mycobacterium tuberculosis ( Mtb ), the causative agent of (TB), has infected over 1.7 billion people worldwide and causes 1.4 million deaths annually. Recently, genome sequence analysis allowed reconstruction complex (MTBC) evolution, with identification seven phylogeographic lineages: four referred to as evolutionarily “ancient”, three “modern”. The MTBC strains belonging “modern” lineages appear show enhanced virulence that may have warranted improved transmission in humans...
ABSTRACT The rapid diagnosis of tuberculosis (TB) and the detection drug-resistant Mycobacterium strains are critical for successful public health interventions. Therefore, TB requires availability diagnostic tools that allow M. drug resistance in clinical samples. Here, we performed a multicenter study to evaluate performance Seegene Anyplex MTB/NTM MDR-TB assay, new molecular method based on multiplex real-time PCR system, complex (MTBC), nontuberculous mycobacteria (NTM), genetic...
Weather and the susceptibility of children to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is still a debated question currently hot topic, particularly in view important decisions regarding opening schools. Therefore, we performed this prospective analysis anti-SARS-CoV-2 immunoglobulin G (IgG) antibodies with known household exposure SARS-CoV-2 compared their IgG status other adults exposed index case same household. A total 30 families documented COVID-19 were...
Genotyping of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been instrumental in monitoring viral evolution and transmission during the pandemic. The quality sequence data obtained from these genotyping efforts depends on several factors, including quantity/integrity input material, technology, laboratory-specific implementation. current lack guidelines for SARS-CoV-2 leads to inclusion error-containing genome sequences genomic epidemiology studies. We aimed establish...
Background. The profile of cellular immunological responses children across the spectrum COVID-19, ranging from acute SARS-CoV-2 infection to full recovery or Long COVID, has not yet been fully investigated. Methods. We examined and compared cytokines in sera cell subsets peripheral blood mononuclear cells (B regulatory T lymphocytes) collected four distinct groups children, distributed as follows: younger than 18 years age with either (n = 49); recovered COVID-19 32); persistent symptoms...
Rationale In 2016, a new interferon-gamma release assay (IGRA) was introduced, QuantiFERON-TB Gold Plus (QFT-Plus), claimed to have improved sensitivity in active tuberculosis (TB). Objectives This study aimed determine the performance of QFT-Plus, compared with previous generation IGRAs and tuberculin skin test (TST), children TB Europe. Methods Multicentre, ambispective cohort within Paediatric Tuberculosis Network European Trials Group (ptbnet), dedicated paediatric research network...
ABSTRACT A total of 750 clinical yeast isolates were evaluated by two identification systems, VITEK 2 and RapID Yeast Plus, using sequence analysis the rRNA gene internal transcribed spacer regions as reference method. The systems correctly identified 737 (98.2%) 716 (95.5%) isolates, respectively.
To evaluate the accuracy of QuantiFERON-TB Gold assay (QFT-IT) in children with suspected active or latent TB infection (LTBI).A retrospective study was conducted on 621 (0-14 years old) evaluated for disease. Following clinical assessment, were tested QFT-IT assay.Among 140 suspects, we identified 19 cases The overall sensitivity 87.5%, ranging from 62.5% 25-36 months old to 100% older than 49 months. specificity 93.6%. Among 481 LTBI screening, 38 scored positive and all but 2 had at least...
Compared to its predecessor QuantiFERON-TB Gold In Tube (QFT-IT), Plus (QFT-Plus) contains an additional antigen tube (TB2), stimulating both CD4 + and CD8 T cells. The ability discriminate responses is suggested be useful in differentiating stages of Mycobacterium tuberculosis infection. While QFT-Plus has already been evaluated adults, there are not enough data children for suspected active (TB) or latent TB infection (LTBI).
Abstract There is increasing reporting by patients’ organization and researchers of long covid (or post-acute sequelae SARS-CoV-2 - PASC), characterized symptoms such as fatigue, dyspnea, chest pain, cognitive sleeping disturbances, arthralgia decline in quality life. Immune system dysregulation with a hyperinflammatory state, direct viral toxicity, endothelial damage microvascular injury have been proposed pathologenic mechanisms. Recently, cohorts children PASC reported Italy, Sweden...