A5021698864- Marine Invertebrate Physiology and Ecology
- Hippo pathway signaling and YAP/TAZ
- Planarian Biology and Electrostimulation
- Air Quality and Health Impacts
- Marine Ecology and Invasive Species
- Toxic Organic Pollutants Impact
- Risk and Safety Analysis
- Probabilistic and Robust Engineering Design
- SARS-CoV-2 and COVID-19 Research
- Effects and risks of endocrine disrupting chemicals
- Marine Toxins and Detection Methods
- Nanoparticle-Based Drug Delivery
- Indoor Air Quality and Microbial Exposure
- Protein Interaction Studies and Fluorescence Analysis
- Autophagy in Disease and Therapy
- Erythrocyte Function and Pathophysiology
- SARS-CoV-2 detection and testing
- Invertebrate Immune Response Mechanisms
- Advanced Clustering Algorithms Research
- Potato Plant Research
- Natural Language Processing Techniques
- Data-Driven Disease Surveillance
- Classical Antiquity Studies
- Computational Drug Discovery Methods
- Machine Learning in Bioinformatics
University of Geneva
2011-2025
Sophia Genetics (Switzerland)
2020-2024
University of Lausanne
2024
University of Alabama at Birmingham
2017
Google (United States)
2015
University of Michigan
2007-2012
École Polytechnique Fédérale de Lausanne
2005
University Hospital of Geneva
2003
Abstract Polyps of the cnidarian Hydra maintain their adult anatomy through two developmental organizers, head organizer located apically and foot basally. The is made antagonistic cross-reacting components, an activator, driving apical differentiation inhibitor, preventing ectopic formation. Here we characterize inhibitor by comparing planarian genes down-regulated when β-catenin silenced to displaying a graded apical-to-basal expression up-regulation during regeneration. We identify Sp5 as...
After bisection, Hydra polyps regenerate their head from the lower half thanks to a head-organizer activity that is rapidly established at tip. Head regeneration also highly plastic as both wild-type and epithelial (that lack interstitial cell lineage) can head. In context, we previously showed after mid-gastric large subset of cells undergo apoptosis, inducing compensatory proliferation surrounding progenitors. This asymmetric process necessary sufficient launch regeneration. The apoptotic...
Evolutionary studies benefit from deep sequencing technologies that generate genomic and transcriptomic sequences a variety of organisms. Genome RNAseq have complementary strengths. In this study, we present the assembly most complete Hydra transcriptome to date along with comparative analysis specific features genome-predicted transcriptomes currently available in freshwater hydrozoan vulgaris. To produce an accurate extensive transcriptome, combined Illumina 454 Titanium reads, giving...
ABSTRACT Hydra possesses three distinct stem cell populations that continuously self-renew and prevent aging in vulgaris. However, sexual animals from the H. oligactis cold-sensitive strain Ho_CS develop an phenotype upon gametogenesis induction, initiated by loss of interstitial cells. Animals stop regenerating, lose their active behaviors die within 3 months. This is not observed cold-resistant Ho_CR. To dissect mechanisms aging, we compared self-renewal epithelial cells these two strains...
Genotyping of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been instrumental in monitoring viral evolution and transmission during the pandemic. The quality sequence data obtained from these genotyping efforts depends on several factors, including quantity/integrity input material, technology, laboratory-specific implementation. current lack guidelines for SARS-CoV-2 leads to inclusion error-containing genome sequences genomic epidemiology studies. We aimed establish...
Hydra continuously differentiates a sophisticated nervous system made of mechanosensory cells (nematocytes) and sensory-motor ganglionic neurons from interstitial stem cells. However, this dynamic adult neurogenesis is dispensable for morphogenesis. Indeed animals depleted their progenitors lose active behaviours but maintain developmental fitness, regenerate bud when force-fed. To characterize the impact loss in Hydra, we first performed transcriptomic profiling at five positions along body...
Abstract Hydrophobic proteins are difficult to analyze by two‐dimensional electrophoresis (2‐DE) because of their intrinsic tendency self‐aggregate during the first dimension (isoelectric focusing, IEF) or equilibration steps. This aggregation renders redissolution for second uncertain and results in reduction number intensity protein spots, undesirable vertical horizontal streaks across gels. Trifluoroethanol (TFE) is traditionally used at high concentration solubilize peptides NMR studies....
Abstract The cnidarian Hydra is a classical model of whole-body regeneration. Historically, apical regeneration has received more attention than its basal counterpart, most studies considering these two regenerative processes independently. We present here transcriptome-wide comparative analysis and after decapitation mid-gastric bisection, augmented with characterization positional cell-type expression patterns in non-regenerating animals. profiles 25’637 transcripts are available on...
Hydra tissues are made from three distinct populations of stem cells that continuously cycle and pause in G2 instead G1. To characterize the role cell proliferation after mid-gastric bisection, we have (i) used flow cytometry classical markers to monitor modulations, (ii) quantified transcriptomic regulations 202 genes associated with during head foot regeneration, (iii) compared impact anti-proliferative treatments on regeneration efficiency. We confirm two previously reported events: an...
Phenotypic traits derive from the selective recruitment of genetic materials over macroevolutionary times, and protein-coding genes constitute an essential component these materials. We took advantage recent production genomic scale data sponges cnidarians, sister groups eumetazoans bilaterians, respectively, to date emergence human proteins infer timing acquisition novel through metazoan evolution. Comparing proteomes 23 eukaryotes, we find that 33% have ortholog in nonmetazoan species....
ContextFor the general population, dominant source of exposure to dioxin-like compounds is food. As part University Michigan Dioxin Exposure Study (UMDES), we measured selected polychlorinated dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs), and biphenyls (PCBs) in serum 946 subjects who were a representative sample population five counties.Case presentationThe total toxic equivalency (TEQ; based on 2005 World Health Organization factors) from index case was 211 ppt lipid-adjusted basis,...
An essential dimension of 3D regeneration in adult animals is developmental, with the formation organizers from somatic tissues. These produce signals that recruit surrounding cells and drive restoration missing structures (organs, appendages, body parts). However, even a high regenerative potential, this developmental potential not sufficient to achieve as homeostatic conditions at time injury need be “pro-regenerative”. In Hydra, we identified four distinct properties provide...
ABSTRACT The freshwater cnidarian polyp named Hydra , which can be mass-cultured in the laboratory, is characterized by a highly dynamic homeostasis with continuous self-renewal of its three adult stem cell populations, epithelial cells from epidermis, gastrodermis, and multipotent interstitial cells, provide nervous system, gland germ cells. Two unusual features characterize these that cannot replace each other, they all avoid G1 to pause G2, two populations are concomitantly...
Abstract Hydra exhibits a negligible senescence as its epithelial and interstitial stem cell populations continuously divide. Here we identified two H. oligactis strains that respond differently to loss. Cold-resistant (Ho_CR) animals adapt remain healthy while cold-sensitive (Ho_CS) ones die within three months, after their cells lose selfrenewal potential. In Ho_CS but not in Ho_CR animals, the autophagy flux is deficient, characterized by low induction upon starvation, proteasome...
e15063 Background: Blood plasma cell-free DNA (cfDNA) testing of cancer patients allows for genomic profiling when tissue sampling is too invasive or insufficient quality. This approach has demonstrated clinical utility to identify treatment targets, screen resistance and monitor disease burden. MSK-ACCESS (Memorial Sloan Kettering – Analysis Circulating cfDNA Evaluate Somatic Status) a validated, high sensitivity assay employed at MSK biologically actionable tumor alterations in care....
Olfactory sensitivity to odorant molecules is a complex biological function influenced by both endogenous factors, such as genetic background and physiological state, exogenous environmental conditions. In animals, this vital ability mediated olfactory sensory neurons (OSNs), which are distributed across several specialized subsystems depending on the species. Using phosphorylation of ribosomal protein S6 (rpS6) in OSNs following stimulation, we developed an ex vivo assay allowing...