A5033903010- Osteoarthritis Treatment and Mechanisms
- Knee injuries and reconstruction techniques
- Total Knee Arthroplasty Outcomes
- Periodontal Regeneration and Treatments
- Proteoglycans and glycosaminoglycans research
- Orthopaedic implants and arthroplasty
- Silk-based biomaterials and applications
- Cell Adhesion Molecules Research
- Erythrocyte Function and Pathophysiology
- Fungal and yeast genetics research
- Wnt/β-catenin signaling in development and cancer
- Heat shock proteins research
- Telomeres, Telomerase, and Senescence
- bioluminescence and chemiluminescence research
- Nutrition and Health in Aging
- SARS-CoV-2 detection and testing
- Alzheimer's disease research and treatments
- Skin and Cellular Biology Research
- Marine Invertebrate Physiology and Ecology
- Inflammatory mediators and NSAID effects
- Nanoplatforms for cancer theranostics
- Frailty in Older Adults
- Injection Molding Process and Properties
- Semantic Web and Ontologies
- Advanced materials and composites
Universität für Weiterbildung Krems
2015-2025
NanoTecCenter Weiz Forschungsgesellschaft (Austria)
2024
Universitätsklinikum Krems
2013-2023
University of Geneva
2009-2020
Klinikum Fulda
2019
John Wiley & Sons (United States)
2019
Österreichischer Rundfunk
2012
NCCR Chemical Biology - Visualisation and Control of Biological Processes Using Chemistry
2004-2010
University of Bonn
1997-2001
Abstract Until recently, chemiluminescence cell images could only be obtained using luciferase‐activated probes. Moreover, microscopy cell‐imaging has not been demonstrated for natively expressed enzymes like cathepsin B. Herein, we describe the design, synthesis, and evaluation of first probe detection imaging The activation mechanism relies on release a dioxetane intermediate, which undergoes chemiexcitation to emit green light with high efficiency under physiological conditions. Using...
Abstract Hop/Stip1/Sti1 is thought to be essential as a co-chaperone facilitate substrate transfer between the Hsp70 and Hsp90 molecular chaperones. Despite this proposed key function for protein folding maturation, it not in number of eukaryotes bacteria lack an ortholog. We set out identify characterize its eukaryote-specific function. Human cell lines budding yeast with deletions Hop/Sti1 gene display reduced proteasome activity due inefficient capping core particle regulatory particles....
Chemiluminescence offers advantages over fluorescence for bioimaging, since an external light source is unnecessary with chemiluminescent agents.
Abstract Until recently, chemiluminescence cell images could only be obtained using luciferase‐activated probes. Moreover, microscopy cell‐imaging has not been demonstrated for natively expressed enzymes like cathepsin B. Herein, we describe the design, synthesis, and evaluation of first probe detection imaging The activation mechanism relies on release a dioxetane intermediate, which undergoes chemiexcitation to emit green light with high efficiency under physiological conditions. Using...
The pleckstrin-homology-domain-containing protein PLEKHA7 was recently identified as a linking the E-cadherin-p120 ctn complex to microtubule cytoskeleton. Here we characterize expression, tissue distribution and subcellular localization of by immunoblotting, immunofluorescence microscopy, immunoelectron northern blotting in mammalian tissues. Anti-PLEKHA7 antibodies label junctional regions cultured kidney epithelial cells major polypeptides M(r) approximately 135 kDa 145 immunoblotting...
Osteoarthritis (OA) is described by an imbalance between anabolic and catabolic processes in the affected joint. This dysregulation of metabolism affects not only chondrocytes within cartilage tissue but also cells synovial membrane across border An important factor OA low viscosity fluid. High-molecular-weight hyaluronic acid (HA) can be used to increase reduce inflammatory processes. The purpose was establish vitro inflammation model evaluate effects high-molecular-weight HA a...
Abstract Background: Minced Cartilage is increasingly used for treating focal acetabular and femoral cartilage defects. In this procedure, autologous harvested from the neck or chondral flap, minced into small pieces re-implanted defect. The question whether already a standardized procedure. Methods: A systematic literature review was conducted in PubMed, Embase, Google Scholar to include all studies (clinical trials, animal basic research) investigating treatment of Subsequently, following...
In various species, thyrotropin (TSH) is known to stimulate both differentiation and proliferation of thyroid follicle cells. This cell type has also been shown express members the Alzheimer amyloid precursor (APP) protein family release secretory N-terminal domain APP (sAPP) in a TSH-dependent fashion. this study on binding surfaces, exogenously added recombinant sAPP stimulated phosphorylation mediated by mitogen-activated kinase effectively evoked rat epithelial line FRTL-5. To see...
Articular cartilage regeneration is insufficient to restore sports injuries or defects that can occur from trauma. Treatment options for repair include autologous chondrocyte implantation (ACI) by isolation, expansion, and reimplantation of healthy donor chondrocytes. Chondrocyte expansion onto 2D substrates leads dedifferentiation loss the cellular phenotype. We aimed overcome state biochemical stimuli with platelet derivatives such as platelet-rich plasma (PRP) hyperacute serum (HAS)...
Partial knee replacement and hemiarthroplasty are some of the orthopedic procedures resulting in a metal on cartilage interface. As implant material, CoCrMo based alloys commonly used. The aim present study is to assess role biotribocorrosion CoCrMo-cartilage interface with an emphasis release during sliding contact. experiments were performed under controlled electrochemical conditions using floating cell three electrode set up coupled microtribometer. Throughout experiment coefficient...
The study aimed to compare the effect of mincing bovine articular cartilage with different shaver blades on chondrocyte viability. Bovine was harvested either a scalpel or three (2.5 mm, 3.5 4.2 mm) from commercially available shaver. then minced into fragments smaller than 1 mm3 scalpel. All four conditions were cultivated in culture medium for seven days. After Day and 7, following measurements performed: metabolic activity, RNA isolation, gene expression anabolic (COL2A1 ACAN) catabolic...
Objective Matrix-assisted autologous chondrocyte implantation is frequently applied to replace damaged cartilage in order support tissue regeneration or repair and prevent progressive degradation osteoarthritis. Its application, however, limited primary defects contraindicated the case of osteoarthritis that partially ascribed dedifferentiation phenotype alterations chondrocytes obtainable from patients’ biopsies. The differentiation state reflected at level structural gene (COL2A1, ACAN,...
Abstract Hybrid components of wood-based materials offer a high potential for automotive lightweight applications. To investigate the bending behavior hybrid aluminum-wood plates, commercial 1-mm-thick sheets EN AW-6016-T4 aluminum alloy were adhesive-bonded with 4.2-mm-thick plates birch wood. Orientations wood fibers parallel (longitudinal) as well perpendicular (transverse) to rolling direction sheet and three different moisture contents plate considered. The subjected three-point at room...
Yeast cells represent a powerful model system in cell biology mainly due to their amenability genetic manipulations. Increasingly, studies focus on mutant genes resulting alterations of cellular structures and organelles. To ascertain the phenotypic changes involved, it is often desirable use resolving power electron microscopy. In contrast higher eukaryotic cells, yeast are particularly difficult preserve presence thick wall that acts as barrier against diffusion fixatives. Although several...
ABSTRACT Recently we generated keratin 10 knockout mice which provided a valuable model for the dominantly inherited skin disorder epidermolytic hyperkeratosis. Here investigated molecular basis their phenotype. Hetero- and homozygotes expressed truncated peptide has been identified directly by microsequencing. Epitope mapping of monoclonal antibodies to 10T enabled us study its distribution relative 6, is highly in mice, double-immunogold electron microscopy. This revealed that was...
Matrix-assisted autologous chondrocyte transplantation (MACT) for focal articular cartilage defects often fails to produce adequate cartilage-specific extracellular matrix in vitro and upon results fibrocartilage due dedifferentiation during cell expansion. This study aimed redifferentiate the chondrocytes through supplementation of blood-products, such as hyperacute serum (HAS) platelet-rich plasma (PRP) vitro. Dedifferentiated monolayer embedded onto collagen type I hydrogels were...