The development of highly sensitive fluorescent probes in combination with innovative optical techniques is a promising strategy for intravital noninvasive quantitative imaging. Cyanine fluorochromes belong to superfamily dyes that have attracted substantial attention probe design molecular We developed novel paradigm introduce Turn-ON mechanism cyanine molecules, based on distinctive change their π-electrons system. Our new fluorochrome synthesized through simple two-step procedure and has...

Smart polymers are special kinds of polymeric molecules that respond to external stimuli. We have developed a novel smart polymer designed sequentially disassemble into its building blocks upon initiation by triggering event at the head. The structure is based on polyurethane backbone disassembles through domino-like, 1,6-elimination and decarboxylation reactions. synthesized self-immolative amplifies single cleavage reaction multiple release fluorogenic confirmed head-to-tail disassembly...

Chemiluminescence probes are considered to be among the most sensitive diagnostic tools that provide high signal-to-noise ratio for various applications such as DNA detection and immunoassays. We have developed a new molecular methodology design foresee light-emission properties of turn-ON chemiluminescence dioxetane suitable use under physiological conditions. The is based on incorporation substituent benzoate species obtained during chemiexcitation pathway Schaap's adamantylidene-dioxetane...

Chemiluminescent luminophores are considered as one of the most sensitive families probes for detection and imaging applications. Due to their high signal-to-noise ratios, with near-infrared (NIR) emission particularly important in vivo use. In addition, light such long wavelength has significantly greater capability penetration through organic tissue. So far, only a few reports have described use chemiluminescence systems imaging. Such always based on an energy-transfer process from...

(Bioiso)steering in a new direction: A modular synthesis of reagents (e.g. sodium difluoroethylsulfinate) that can be used for the direct incorporation difluoroalkyl groups onto heterocycles is reported. The scope and generality exemplified with difluoroethyl group proof principle shown general fluorinated heteroarylether bioisosteres. As service to our authors readers, this journal provides supporting information supplied by authors. Such materials are peer reviewed may re-organized online...

Chemiluminescence is among the most sensitive methods for achieving a high signal-to-noise ratio in various chemical and biological applications. We have developed modular practical synthetic route preparation of turn-ON fluorophore-tethered dioxetane chemiluminescent probes. The emission probes was significantly amplified through an energy-transfer mechanism under physiological conditions. Two were composed with green near-infrared (NIR) fluorescent dyes tethered to Schaap's dioxetane....

Formaldehyde (FA) is a common environmental toxin that also produced naturally in the body through wide range of metabolic and epigenetic processes, motivating development new technologies to monitor this reactive carbonyl species (RCS) living systems. Herein, we report pair first-generation chemiluminescent probes for selective formaldehyde detection. Caging phenoxy-dioxetane scaffolds bearing different electron-withdrawing groups with general 2-aza-Cope trigger provides 540 700 (CFAP540...

Selective and sensitive molecular probes for hydrogen peroxide (H2 O2 ), which plays diverse roles in oxidative stress redox signaling, are urgently needed to investigate the physiological pathological effects of H2 . A lack reliable tools vivo imaging has hampered development mediated therapeutics. By combining a specific tandem Payne/Dakin reaction with chemiluminescent scaffold, -CL-510 was developed as highly selective probe detection both vitro vivo. rapid 430-fold enhancement...

Identification and characterization of bacterial species in clinical industrial settings necessitate the use diverse, labor-intensive, time-consuming protocols as well utilization expensive high-maintenance equipment. Furthermore, while cutting-edge identification technologies such mass spectrometry PCR are highly effective identifying pathogens, they fall short providing additional information for bacteria not present databases upon which these methods rely. In response to challenges, we a...

Docking the tails: The triggering of a single cleavage at core new class dendrimers (see, for example, second generation dendron) results in spontaneous chain fragmentation and release all tail (reporter) units. Such may be applied as general platform prodrugs or sensor molecules enzymatic activity. Dendrimers are perfectly cascade-branched, highly defined, synthetic macromolecules, which characterized by combination high number functional groups compact molecular structure.1 concept...

This paper describes the substrate specificity, synthetic scope, and efficiency of aldolase catalytic antibodies 38C2 33F12. These use enamine mechanism common to natural Class I enzymes. Substrates for these catalysts, 23 donors 16 acceptors, have been identified. The aldol acceptor specificity is expected be much broader than that defined here since all aldehydes tested, with exception polyhydroxylated aldehydes, were substrates antibodies. 33F12 shown catalyze intermolecular...

The voltage-dependent M-type potassium current (M-current) plays a major role in controlling brain excitability by stabilizing the membrane potential and acting as brake for neuronal firing. KCNQ2/Q3 heteromeric channel complex was identified molecular correlate of M-current. Furthermore, KCNQ2 KCNQ3 α subunits are mutated families with benign familial neonatal convulsions, form epilepsy. Enhancement currents may provide an important target antiepileptic drug development. Here, we show that...

Self-immolative dendrimers have recently been developed and introduced as a potential platform for multi-prodrug. These unique structural can release all of their tail units, through self-immolative chain fragmentation, which is initiated by single cleavage at the dendrimer's core. Incorporation drug molecules units an enzyme substrate trigger generate multi-prodrug unit that will be activated with enzymatic cleavage. We synthesized first generation dendritic prodrugs doxorubicin...

The detection of chemical or biological analytes upon molecular reactions relies increasingly on fluorescence methods, and there is a demand for more sensitive, specific, versatile fluorescent molecules. We have designed long wavelength fluorogenic probes with turn-ON mechanism based donor–two-acceptor π-electron system that can undergo an internal charge transfer to form new fluorochromes longer systems. Several latent donors multiple acceptor molecules were incorporated into the probe...

Over the last 30 years, quinone-methide elimination has served as a valuable tool for achieving various important molecular functions. Molecular adaptors based on or aza-quinone-methide reactivity have been designed, synthesized, and used in diagnostic probes, amplifiers, drug delivery systems, self-immolative dendritic/polymeric systems. These unique function stable spacers between an enzyme- reagent-responsive group reporter moiety can undergo 1,4-, 1,6-, 1,8-type reactions upon cleavage...

A polymer therapeutic designed for combination anticancer and antiangiogenic therapy inhibited the proliferation of prostate carcinoma cells proliferation, migration, tube-formation endothelial cells. The nanoconjugate was formed from an N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer, bisphosphonate alendronate (for bone targeting), chemotherapy agent paclitaxel (PTX), which is cleaved by cathepsin B (see scheme).

Abstract Singlet oxygen is among the reactive species (ROS) with shortest life‐times in aqueous media because of its extremely high reactivity. Therefore, designing sensors for detection 1 O 2 perhaps one most challenging tasks field molecular probes. Herein, we report a highly selective and sensitive chemiluminescence probe ( SOCL‐CPP ) living cells. The reacts to form dioxetane that spontaneously decomposes under physiological conditions through chemiexcitation pathway emit green light...

Abstract Until recently, chemiluminescence cell images could only be obtained using luciferase‐activated probes. Moreover, microscopy cell‐imaging has not been demonstrated for natively expressed enzymes like cathepsin B. Herein, we describe the design, synthesis, and evaluation of first probe detection imaging The activation mechanism relies on release a dioxetane intermediate, which undergoes chemiexcitation to emit green light with high efficiency under physiological conditions. Using...

A new prodrug system, assembled using a distinctive coumarin linker, was demonstrated to report real-time activation and drug release in vitro.

Signal amplification techniques are broadly used to improve the detection sensitivity of various analytes for diagnostic purposes. We have developed a novel, non-PCR-based modular technique exponential signals that is conveniently performed in an aqueous environment. The based on distinctive dendritic chain reaction (DCR); signal generated upon disassembly self-immolative dendrimer releases chromogenic molecules. Under ideal conditions, single analyte molecule initiates DCR generates strong...

For the first time, science now have a single-entity chemiluminescent luminophore that can serve to prepare effective diagnostic probes evaluate biological processes<italic>in vitro</italic>and<italic>in vivo</italic>.