A5069601930- Chronic Lymphocytic Leukemia Research
- Pancreatic function and diabetes
- Acute Lymphoblastic Leukemia research
- Acute Myeloid Leukemia Research
- Lymphoma Diagnosis and Treatment
- Immunodeficiency and Autoimmune Disorders
- Diabetes and associated disorders
- Neuroblastoma Research and Treatments
- Immune Cell Function and Interaction
- Chronic Myeloid Leukemia Treatments
- Drug Transport and Resistance Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Diabetes Treatment and Management
- Metabolism, Diabetes, and Cancer
- Hemoglobinopathies and Related Disorders
- Peripheral Artery Disease Management
- Diabetes Management and Research
- Neuroendocrine Tumor Research Advances
- Blood disorders and treatments
- Immune Response and Inflammation
- Cell death mechanisms and regulation
- Iron Metabolism and Disorders
- Platelet Disorders and Treatments
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
Ghent University Hospital
2015-2025
Ghent University
2016-2025
Cancer Research Institute Ghent
2017-2025
Leiden University Medical Center
2020
École Centrale de Lille
2016
Institut d'électronique de microélectronique et de nanotechnologie
2016
Centre National de la Recherche Scientifique
2016
Université de Lille
2013-2016
University Hospital of Geneva
2002-2015
Université Catholique de Bukavu
2015
Insulin secretion is controlled by a complex set of factors. Although blood glucose levels serve as the major stimulus insulin in mammals, release also modulated amino acids, catecholamines, glucagon, and other, intestinal hormones. The identification factors that modulate production has engendered much interest because their potential importance altered dynamics response to characteristic maturity-onset diabetes mellitus. Decoding glucagon gene uncovered two additional glucagon-like...
Measurable residual disease (MRD) is an important biomarker in acute myeloid leukemia (AML) that used for prognostic, predictive, monitoring, and efficacy-response assessments. The European LeukemiaNet (ELN) MRD Working Party evaluated standardization harmonization of ongoing manner has updated the 2018 ELN recommendations based on significant developments field. New revised were established during in-person online meetings, a 2-stage Delphi poll was conducted to optimize consensus. All are...
Abstract. A potential application of the continuous renal replacement therapies is extracorporeal removal inflammatory mediators in septic patients. Cytokine elimination with has been demonstrated several clinical studies, but so far without important effects on their serum concentrations. Improved knowledge cytokine mechanisms could lead to development more efficient treatment strategies. In present study, 15 patients shock and acute failure were observed during first 24 h venovenous...
Obtaining reliable and reproducible high quality data in multicenter clinical research settings requires design of optimal standard operating procedures. While the need for standardization sample processing analysis is well-recognized, impact handling pre-analytical phase remains underestimated. We evaluated storage time (≈transport time) temperature, type anticoagulant, limited blood volume on reproducibility flow cytometric studies. EDTA Na-Heparin samples processed with EuroFlow bulk...
Measurable residual disease (MRD) quantified by multiparameter flow cytometry (MFC) is a strong and independent prognostic factor in acute myeloid leukemia (AML). However, several technical factors may affect the final read-out of assay. Experts from MRD Working Party European LeukemiaNet evaluated which aspects are crucial for accurate MFC-MRD measurement. Here, we report on agreement, obtained via combination cross-sectional questionnaire, live discussions, Delphi poll. The recommendations...
Truncated glucagon-like peptide (GLP)-1 is a potent incretin. Its synthesis and secretion are modulated by food, but the influence of individual nutrients remains to be established. The hypothesis that protein hydrolysates (peptones) can directly regulate both GLP-1 proglucagon (PG) gene transcription was tested in this study, ex vivo isolated vascularly perfused rat intestine vitro murine enteroendocrine cell line STC-1. Peptones were albumin egg hydrolysate (AEH) meat (MH). We demonstrate...
Circumvention of the p53 tumor suppressor barrier in neuroblastoma is rarely caused by TP53 mutation but might arise from inappropriately increased activity its principal negative regulator MDM2. We show here that targeted disruption p53-MDM2 interaction small-molecule MDM2 antagonist nutlin-3 stabilizes and selectively activates pathway cells with wild-type p53, resulting a pronounced antiproliferative cytotoxic effect through induction G(1) cell cycle arrest apoptosis. A response was...
Ontogenic relationships between the different types of endocrine cells in islets Langerhans were explored by generating transgenic mouse embryos which transcribing glucagon, insulin, or pancreatic polypeptide genes destroyed through promoter-targeted expression diphtheria toxin A chain. Embryos lacking glucagon- insulin-containing did not exhibit alterations development nontargeted islet cell types, whereas gene-expressing also lacked insulin- and somatostatin-containing cells. These results...
The glucagon gene is expressed specifically in the alpha cells of pancreatic islets. We show here that 300 base pairs 5'-flanking region rat gene, linked to a chloramphenicol acetyltransferase reporter plasmid transfected into islet cell lines different hormone-producing phenotypes, directs transcription only glucagon-producing cells. Deletional and linker-scanning mutations DNase I footprinting assays identify three transcriptional control elements within these pairs. Two (G2 G3)...
The developmental origin of the four phenotypically distinct hormone-producing islet cells (insulin, glucagon, somatostatin, pancreatic polypeptide) is unclear. To investigate potential for phenotypic differentiation cells, we prepared several clonal cell lines from a radiation-induced rat tumor and analyzed them insulin, somatostatin gene expression by cDNA hybridization, immunocytochemistry, radioimmunoassay. We found all three genes in parental line mixed variable phenotypes derived line....
BackgroundPredominantly antibody deficiencies (PADs) are the most prevalent primary immunodeficiencies, but their B-cell defects and underlying genetic alterations remain largely unknown.ObjectiveWe investigated patients with PADs for distribution of 41 blood plasma cell (PC) subsets, including subsets defined by expression distinct immunoglobulin heavy chain subclasses.MethodsBlood samples from 139 PADs, 61 common variable immunodeficiency (CVID), 68 selective IgA deficiency (IgAdef), 10...
Guidelines for screening primary immunodeficiencies (PID) are well-defined and several consensus diagnostic strategies have been proposed. These proposals only partially implemented due to lack of standardization in laboratory procedures, particularly flowcytometry. The main objectives the EuroFlow Consortium were innovate thoroughly standardize flowcytometric techniques reliable reproducible diagnosis classification PID lymphoid system. proposed antibody panels comprise one orientation tube...
To assess whether connexin (Cx) expression contributes to insulin secretion, we have investigated normal and tumoral insulin-producing cells for connexins, gap junctions, coupling. We found that the glucose-sensitive of pancreatic islets a rat insulinoma are functionally coupled by junctions made Cx43. In contrast, several lines secreting abnormally do not express Cx43, After correction these defects stable transfection Cx43 cDNA, expressing modest levels coupling, as observed in native...
Analogues of cAMP have been reported to increase insulin mRNA levels in normal rat beta-cells and hamster insulinoma cells (HIT). To define the mechanisms by which modulates gene expression, we first investigated its effects on transcriptional rate HIT cells. Nuclear run-on assays revealed a 4-fold transcription observed as early 1 h after stimulation. characterize cis-acting sequences I promoter trans-acting factors mediating effect transcription, constructed DNA plasmids containing various...
Complex interrelationships exist between the four pancreatic islet cell types and their respective secretory products, insulin, glucagon, somatostatin, polypeptide. These hormones are known to interact with different cells modulate functions. Insulin inhibits glucagon secretion from A both in vivo vitro and, states of insulin deficiency, high levels observed that normalized by replacement. To determine if also regulates biosynthesis, we studied its effects on gene expression. Our studies...
Results of islet Langerhans transplantation have markedly improved in recent years, but most patients still lose insulin independence the long-term. We report herein longest (over 11 years) case after allogeneic transplantation. The subject had a 27-year history type 1 diabetes and received single islet-after-kidney graft 8800 equivalents (IEQ)/kg, pooled from 2 donors. Insulin was discontinued by 3 months posttransplant patient has remained off ever since. Yearly follow-up studies revealed...