A5055108744- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunodeficiency and Autoimmune Disorders
- CAR-T cell therapy research
- Cerebral Venous Sinus Thrombosis
- HIV Research and Treatment
- Acute Ischemic Stroke Management
- Platelet Disorders and Treatments
- Acute Lymphoblastic Leukemia research
- Chronic Myeloid Leukemia Treatments
- Reproductive System and Pregnancy
- Immunotherapy and Immune Responses
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Insect and Pesticide Research
- Insect Utilization and Effects
- Blood disorders and treatments
- Congenital heart defects research
- Coronary Artery Anomalies
- Dermatology and Skin Diseases
- FOXO transcription factor regulation
- Cancer Immunotherapy and Biomarkers
- Congenital Heart Disease Studies
- Cytomegalovirus and herpesvirus research
- Blood groups and transfusion
University of Lisbon
2010-2024
Instituto de Medicina Molecular João Lobo Antunes
2019-2020
Centro Hospitalar Lisboa Norte
2019
<h3>Background</h3> Humoral immunocompetence develops stepwise throughout life and contributes to individual susceptibility infection, immunodeficiency, autoimmunity, neoplasia. Immunoglobulin heavy chain (IgH) isotype serum levels can partly explain such age-related differences, but their relationship with the IgH distribution within memory B-cell (MBC) plasma cell (PCs) compartments remains be investigated. <h3>Objective</h3> We studied of MBCs PCs expressing different isotypes in addition...
Adult B-cell acute lymphoblastic leukemia remains a major therapeutic challenge, requiring better characterization of the molecular determinants underlying disease progression and resistance to treatment. Here, using phospho-flow cytometry approach we show that adult diagnostic specimens display PI3K/Akt pathway hyperactivation, irrespective their BCR-ABL status despite paradoxically high basal expression PTEN, negative regulator pathway. Protein kinase CK2 is known phosphorylate PTEN...
BackgroundPredominantly antibody deficiencies (PADs) are the most prevalent primary immunodeficiencies, but their B-cell defects and underlying genetic alterations remain largely unknown.ObjectiveWe investigated patients with PADs for distribution of 41 blood plasma cell (PC) subsets, including subsets defined by expression distinct immunoglobulin heavy chain subclasses.MethodsBlood samples from 139 PADs, 61 common variable immunodeficiency (CVID), 68 selective IgA deficiency (IgAdef), 10...
// Pedro D. Pereira 1, * , Ana Serra-Caetano Marisa Cabrita 2 Evguenia Bekman 1 José Braga Rino Renè Santus 3 Paulo L. Filipe E. Sousa and João A. Ferreira Instituto de Medicina Molecular, Faculdade da Universidade Lisboa, 1649-028 Portugal Kennedy Institute of Rheumatology, University Oxford, OX3 7FY United Kingdom Muséum National d´Histoire Naturelle, Département RDDM, 75231 Paris, France These authors contributed equally to this work Correspondence to: Ferreira, email: hjoao@fm.ul.pt...
Naïve FoxP3-expressing regulatory T-cells (Tregs) are essential to control immune responses via continuous replenishment of the activated-Treg pool with thymus-committed suppressor cells. The mechanisms underlying naïve-Treg maintenance throughout life in face age-associated thymic involution remain unclear. We found that adults thymectomized early infancy is remarkably well preserved, contrast conventional naïve CD4 T-cells. Naïve-Tregs featured high levels cycling and pro-survival markers,...
Experimental studies suggest inflammation can contribute to blood barrier disruption and brain injury in cerebral venous thrombosis (CVT). We aimed determine whether biomarkers of were associated with the evolution lesions, persistent occlusion or functional outcome patients CVT.Pathophysiology Venous Infarction-Prediction Infarction Recanalization CVT (PRIORITy-CVT) was a multicenter prospective cohort study newly diagnosed CVT. Evaluation neutrophil-to-lymphocyte ratio (NLR) C-reactive...
Abstract Background Elucidating mechanisms of brain damage in cerebral venous thrombosis (CVT) would be instrumental to develop targeted therapies and improve prognosis prediction. Matrix metalloproteinase-9 (MMP-9), a gelatinase that degrades major components the basal lamina, has been associated blood–brain barrier disruption. We aimed assess, patients with CVT, temporal change serum concentrations MMP-9 its association key imaging clinical outcomes. Methods Pathophysiology Venous...
Abstract Follicular helper T cells (Tfh), CD4 lymphocytes critical for efficient antibody responses, have been shown to be key human immunodeficiency virus (HIV)-1 reservoirs. Human virus-2 infection represents a unique naturally occurring model investigating Tfh role in HIV/acquired immune deficiency syndrome, given its slow rate of decline, low undetectable viremia, and high neutralizing titers throughout the disease course. In this study, we investigated, first time, susceptibility HIV-2...
Computational strategies to extract meaningful biological information from multiomics data are in great demand for effective clinical use, particularly complex immune-mediated disorders. Regulatory T cells (Tregs) essential immune homeostasis and self-tolerance, controlling inflammatory autoimmune processes many diseases with a multigenic basis. Here, we quantify the Transcription Factor (TF) differential occupancy landscape uncover Gene Modules governing lineage-committed Tregs human...
Monozygotic twins provide a unique opportunity to better understand complex genetic diseases and the relative contribution of heritable factors in shaping immune system throughout life. Common Variable Immunodeficiency Disorders (CVID) are primary antibody defects displaying wide phenotypic heterogeneity, with monogenic transmission accounting for only minority cases. Here, we report pair monozygotic concordant CVID without family history immunodeficiency. They featured remarkably similar...
The thymus generates a T‐cell lineage dedicated to immune regulation, ‘naturally occurring’ regulatory T cells, best specified by the forkhead family transcription factor Foxp3. Here, we have conducted parallel study in humans and mice where dissected earliest stages of Foxp3 induction during thymocyte development. By analyzing large collection 21 human thymuses show that can be consistently detected CD4 immature single positive thymocytes precede CD4( + )CD8( ) (double positive, DP) stage....
Introdução: A síndrome de DiGeorge/deleção 22q11.2 pode apresentar um grau variável imunodeficiência, condicionando a utilização vacinas vivas. Este estudo teve como objetivo documentar os efeitos adversos vivas e possível relação com alterações imunitárias em crianças parcial.Material Métodos: Foi realizado retrospetivo por revisão dos processos clínicos das deleção do cromossoma fenótipo DiGeorge, seguidos num centro referência imunodeficiências primárias. realizada colheita dados,...
ABTRACT Computational strategies to extract meaningful biological information from multiomics data are in great demand for effective clinical use. This is most relevant immune-mediated disorders, where the combined impact of multiple variants difficult determine. Regulatory T cells (Tregs), particularly those lineage-committed thymus, essential immune homeostasis and self-tolerance, controlling inflammatory autoimmune processes many diseases with a multigenic basis. Here, we quantify...