A5035867136- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Coastal wetland ecosystem dynamics
- Coastal and Marine Dynamics
- Immunotherapy and Immune Responses
- HIV Research and Treatment
- CAR-T cell therapy research
- IL-33, ST2, and ILC Pathways
- HIV/AIDS Research and Interventions
- RNA and protein synthesis mechanisms
- Chromosomal and Genetic Variations
- Molecular Biology Techniques and Applications
- Cytomegalovirus and herpesvirus research
- Flood Risk Assessment and Management
- Tropical and Extratropical Cyclones Research
- Whipple's Disease and Interleukins
- HIV-related health complications and treatments
- Lymphoma Diagnosis and Treatment
- Viral Infections and Immunology Research
- Geological formations and processes
Instituto de Medicina Molecular João Lobo Antunes
2023
University of Lisbon
2012-2022
Germinal center (GC) responses are controlled by T follicular helper (Tfh) and regulatory (Tfr) cells crucial for the generation of high-affinity antibodies. Although biology human circulating tissue Tfh has been established, relationship between blood Tfr defined as CXCR5+Foxp3+ remains elusive. We found that increased in Sjögren syndrome, an autoimmune disease with ongoing GC reactions, especially patients high autoantibody titers, well healthy individuals upon influenza vaccination....
Naïve FoxP3-expressing regulatory T-cells (Tregs) are essential to control immune responses via continuous replenishment of the activated-Treg pool with thymus-committed suppressor cells. The mechanisms underlying naïve-Treg maintenance throughout life in face age-associated thymic involution remain unclear. We found that adults thymectomized early infancy is remarkably well preserved, contrast conventional naïve CD4 T-cells. Naïve-Tregs featured high levels cycling and pro-survival markers,...
Background. Interleukin 22 (IL-22) is emerging as a key cytokine for gut epithelial homeostasis and mucosal repair. Gut disruption hallmark of human immunodeficiency virus (HIV) infection. Here, we investigated IL-22 production integrity in HIV type 1 (HIV-1)–infected individuals receiving long-term antiretroviral therapy (ART). Methods. Biopsy specimens from 37 who underwent colonoscopy primarily cancer screening 17 HIV-1–infected 20 healthy age-matched controls were assessed. Results. We...
Abstract FOXP3-expressing regulatory T cells (Treg) are essential for the prevention of autoimmunity and were shown to be reduced and/or dysfunctional in several autoimmune diseases. Although Treg-based adoptive transfer represents a promising therapy, large cell number required achieve clinical efficacy constitutes an important limitation. Therefore, novel strategies generate bona fide vitro–induced Treg (iTreg) critical. In this study, we report that human memory CD4 can efficiently...
ABSTRACT A unique HIV-host equilibrium exists in untreated HIV-2-infected individuals. This is characterized by low to undetectable levels of viremia throughout the disease course, despite establishment disseminated HIV-2 reservoirs at comparable those observed HIV-1 infection. Although clinical spectrum similar two infections, infection associated with a much lower rate CD4 T-cell decline and has limited impact on mortality infected adults. Here we investigated human thymus, primary organ...
Commitment to the CD4+ or CD8+ T cell lineages is linked acquisition of a functional program broadly defined by helper and cytotoxic properties, respectively. The mechanisms underlying these processes in human thymus remain largely unclear. Moreover, recent thymic emigrants are thought have some degree plasticity, which may be important for shaping immune system adjustment specific peripheral needs. We show here that IL-4 induces proliferation-independent
Human Endogenous Retroviruses (HERVs) constitute up to 8% of the human genome and have been emerging as important modulators immune system, being associated with cancer, autoimmunity infectious diseases. Here, we investigated expression three HERV families in thymus. HERV-K, -W, -R envelope ( env ) HERV-K gag transcriptional levels were quantified main thymocyte subsets, thymic epithelial cells (TECs), B myeloid populations, Env protein was studied tissue. We found that mRNA decreased T cell...
La présente étude examine le comportement morphologique d'une embouchure tidale éphémère, depuis deux semaines après son ouverture artificielle jusqu'à comblement naturel huit mois plus tard.Des levés topo-hydrographiques mensuels permettent de discuter l'évolution au cours cette période la position longitudinale du chenal principal, l'aire section mouillée principal et des chenaux internes deltas flot jusant.Le calcul dérive littorale en face l'embouchure (grâce un modèle houle régional à...
The immunohistochemistry (IHC) protocol is used to showcase the location of specific proteins in relation arquitecture tissue. In this method, we use a primary antibody which binds antigen protein interest. This has host and can be ready or undiluted. secondary an anti-host IgG will bind 1. It's also conjugated with Horseradish Peroxidase (HRP). HRP react Hydrogen Peroxide substract DAB solution, oxidizing DAB, creating brown deposit on Depending protein, have staining nuclei, cytoplasm...