A5054844397- Nicotinic Acetylcholine Receptors Study
- Ion channel regulation and function
- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Family and Matrimonial Law
- Cholinesterase and Neurodegenerative Diseases
- Theology and Canon Law Studies
- Photoreceptor and optogenetics research
- Comparative constitutional jurisprudence studies
- Human Rights and Immigration
- Genetic and rare skin diseases.
- Cutaneous lymphoproliferative disorders research
- Drug-Induced Adverse Reactions
- Comparative International Legal Studies
- Autoimmune Bullous Skin Diseases
- Plant tissue culture and regeneration
- Legal processes and jurisprudence
- Skin and Cellular Biology Research
- Dermatologic Treatments and Research
- Eosinophilic Disorders and Syndromes
- Nail Diseases and Treatments
- Skin Diseases and Diabetes
- Chronic Lymphocytic Leukemia Research
- Oral and gingival health research
- Insect and Pesticide Research
University of Alicante
1984-2023
Universitat de Miguel Hernández d'Elx
2004-2020
Instituto de Neurociencias
1996-2019
Universidad de Navarra
2001-2019
Complejo Hospitalario de Navarra
2012-2018
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2017
University of Milan
1986-2014
Hospital Universitari de Vic
2012-2013
Baylor College of Medicine
2012
Azienda Ospedaliera San Gerardo
2009
Confocal laser-scanned microscopy and long-wavelength calcium (Ca 2+ ) indicators were combined to monitor both sustained rapidly dissipating Ca gradients in voltage-clamped sympathetic neurons isolated from the bullfrog. After a brief activation of voltage-dependent channels, spreads inwardly, reaches center these spherical cells about 300 milliseconds. Although redistribution bulk cytosol could be accounted for with radial diffusion model, local nonlinearities, suggesting either nonuniform...
In Caenorhabditis elegans, the ric-3 gene is required for maturation of multiple nicotinic acetylcholine receptors (nAChRs), whereas other neurotransmittergated channels expressed within same cells are unaffected by presence RIC-3. Here we show that RIC-3 a member conserved family with representatives in both vertebrates and invertebrates. All members this have two transmembrane domains followed coiled-coil domain. Expression human homolog, hric3, like C. elegans ric-3, enhances DEG-3/DES-2,...
Sixty-seven out of 100 kidney transplant recipients treated with cyclosporin and methylprednisolone were evaluated for the presence dermatological manifestations. Only 2 patients had no lesions; 80% iatrogenic lesions, 38% infectious, 13% miscellaneous, 3% cancerous while 28% cutaneous manifestations related to previous uremic state. Most lesions concerned pilosebaceous unit: hypertrichosis (60%), epidermal cysts (28%), pilar keratosis (21%), acne (15%), folliculitis (12%) sebaceous...
Abstract: Neuronal nicotinic acetylcholine receptors from bovine adrenomedullary chromaffin cells play a primary role in triggering catecholamine secretion. In the present study, their constituent subunits were characterized. addition to α3 subunit, which we have previously cloned, presence of α5 and β4 but not β2 was detected by reverse transcription‐PCR analysis mRNA adrenal medulla. situ hybridization indicated that α3, α5, are coexpressed all cells. The structure determined functional...
BackgroundPredominantly antibody deficiencies (PADs) are the most prevalent primary immunodeficiencies, but their B-cell defects and underlying genetic alterations remain largely unknown.ObjectiveWe investigated patients with PADs for distribution of 41 blood plasma cell (PC) subsets, including subsets defined by expression distinct immunoglobulin heavy chain subclasses.MethodsBlood samples from 139 PADs, 61 common variable immunodeficiency (CVID), 68 selective IgA deficiency (IgAdef), 10...
The ric-3 gene is required for maturation of nicotinic acetylcholine receptors in Caenorhabditis elegans. human homolog RIC-3, hRIC-3, enhances expression alpha7 Xenopus laevis oocytes, whereas it totally abolishes alpha4beta2 and 5-HT3 serotonergic receptors. Both the N-terminal region which contains two transmembrane segments, C-terminal are needed these differential effects. hRIC-3 inhibits receptor by hindering export mature to cell membrane. By using chimeric proteins made receptors, we...
Abstract Chromaffin cells from the bovine adrenal medulla express α‐bungarotoxin‐sensitive acetylcholine receptors whose subunit composition is unknown. Northern blot analysis showed that α7 subunit, a main component of these in avian and rat brain, expressed chromaffin cells. The cDNA this was cloned by polymerase chain reaction amplification RNA for detailed characterization structure function. protein‐coding region revealed 92% amino acid sequence identity to 89% chicken subunits....
Binding of agonists to nicotinic acetylcholine receptors generates a sequence changes that activate cation-selective conductance. By measuring electrophysiological responses in chimeric alpha7/alpha3 expressed Xenopus oocytes, we have showed the involvement M2-M3 loop coupling agonist binding channel gate. An aspartate residue therein, Asp-266 alpha7 subunit, was identified by site-directed mutagenesis as crucial, since mutants at this position exhibited very poor functional three different...
Abstract A simple procedure has been worked out for the freeze‐preservation of rice ( Oryza sativa L.) cells grown in suspension culture. The protocol differs some interesting aspects from those established other organisms. peculiar feature this is that growth freeze‐recovered resumes after an extremely short lag period 2–8 days and proceeds with a rate identical to untreated cells. This, together data obtained viability tests, rules possibility selection freeze‐resistant mutant may occur,...
Ginseng saponins, major active components of ginseng root used by folk medicine in the treatment various diseases, produce multiple pharmacological responses having many effects on central and peripheral nervous system. Specifically, ginsenoside Rg<sub>2</sub>has been shown to block nicotinic acetylcholine receptors bovine chromaffin cells. We have studied effect Rg<sub>2</sub> different types human neuronal (nAChRs), both homomeric heteromeric, expressed in<i>Xenopus</i> oocytes. did not...
Acquired tufted angioma is a benign, slowly progressive with typical histologic pattern that was first described by Wilson Jones. The clinical, histopathologic and transmission electron microscopic features of case this rare condition are presented. Angioblastoma Nagakawa clinically histopathologically the same as acquired angioma.
Among 186 patients with multiple myeloma (MM), five women were diagnosed as having MM without M-component in serum and or urine at the diagnosis along evolution. Bone marrow plasmacytosis greater than 30% was found all bone x-rays showed lytic lesions but one case, osteoporosis all, pathologic fractures two. Serum electrophoresis a striking hypogammaglobulinemia polyclonal immunoglobulin levels markedly reduced. The immunofluorescence of plasma cells positive for monoclonal light chain...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRole of Two Acetylcholine Receptor Subunit Domains in Homomer Formation and Intersubunit Recognition, as Revealed by .alpha.3 .alpha.7 ChimerasMiguel Garcia-Guzman, Francisco Sala, Salvador Antonio Campos-Caro, Manuel CriadoCite this: Biochemistry 1994, 33, 50, 15198–15203Publication Date (Print):December 1, 1994Publication History Published online1 May 2002Published inissue 1 December...
Abstract Rice blast disease, caused by the fungus Pyricularia grisea (Cooke) Sacc., is responsible for considerable damages in rice crops Italy and other parts of world. This study was conducted order to investigate genetic structure a P. population Po area, largest area Italy. leaves showing symptoms were collected three successive years (1998–2000) 43 monoconidial culture samples isolated from infected leaves. Fungal DNAs obtained mycelia. Moreover, six additional DNA representative five...