A5064083413- Immunodeficiency and Autoimmune Disorders
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Allergic Rhinitis and Sensitization
- Blood disorders and treatments
- Epigenetics and DNA Methylation
- Asthma and respiratory diseases
- SARS-CoV-2 and COVID-19 Research
- Cytomegalovirus and herpesvirus research
- Monoclonal and Polyclonal Antibodies Research
- Chronic Lymphocytic Leukemia Research
- Food Allergy and Anaphylaxis Research
- Platelet Disorders and Treatments
- CAR-T cell therapy research
- Dermatology and Skin Diseases
- Diabetes and associated disorders
- COVID-19 Clinical Research Studies
- Celiac Disease Research and Management
- Cancer Immunotherapy and Biomarkers
- Immune responses and vaccinations
- Blood groups and transfusion
- Microscopic Colitis
- Gut microbiota and health
- Cell Adhesion Molecules Research
Monash University
2016-2025
Alfred Health
2018-2025
Jeffrey Modell Foundation
2018-2025
Erasmus MC
2014-2025
Erasmus University Rotterdam
2013-2025
The Alfred Hospital
2018-2024
Australian Regenerative Medicine Institute
2020-2024
Amsterdam University Medical Centers
2021-2024
University of Amsterdam
2021
Henry M. Jackson Foundation
2021
The CD19 protein forms a complex with CD21, CD81, and CD225 in the membrane of mature B cells. Together B-cell antigen receptor, this signals cell to decrease its threshold for activation by antigen.We evaluated four patients from two unrelated families who had increased susceptibility infection, hypogammaglobulinemia, normal numbers cells blood. We found mutation gene all patients. their first-degree relatives was sequenced, flow-cytometric immunophenotyping cells, immunohistochemical...
Antibody deficiencies constitute the largest group of symptomatic primary immunodeficiency diseases. In several patients, mutations in CD19 have been found to underlie disease, demonstrating critical role for protein encoded by this gene antibody responses; functions a complex with CD21, CD81, and CD225 signal B cell receptor upon antigen recognition. We report here patient severe nephropathy profound hypogammaglobulinemia. The was characterized decreased memory numbers, impaired specific...
Memory B cells specific for SARS-CoV-2 spike and nucleocapsid proteins persist in peripheral blood after recovery from COVID-19.
<h3>Background</h3> Humoral immunocompetence develops stepwise throughout life and contributes to individual susceptibility infection, immunodeficiency, autoimmunity, neoplasia. Immunoglobulin heavy chain (IgH) isotype serum levels can partly explain such age-related differences, but their relationship with the IgH distribution within memory B-cell (MBC) plasma cell (PCs) compartments remains be investigated. <h3>Objective</h3> We studied of MBCs PCs expressing different isotypes in addition...
Secretory immunoglobulin A (SIgA) enhances host-microbiota symbiosis, whereas SIgM remains poorly understood. We found that gut IgM+ plasma cells (PCs) were more abundant in humans than mice and clonally related to a large repertoire of memory B disseminated throughout the intestine but rare systemic lymphoid organs. In addition sharing gut-specific gene signature with IgA+ cells, some clonotypes switched IgA response T cell-independent or cell-dependent signals. These signals induced IgM...
Umbilical cord blood (UCB) is commonly used in epigenome-wide association studies of prenatal exposures. Accounting for cell type composition critical such as it reduces confounding due to the specificity DNA methylation (DNAm). In absence sorting information, statistical methods can be applied deconvolve heterogeneous mixtures. Among these methods, reference-based approaches leverage age-appropriate cell-specific DNAm profiles estimate cellular composition. UCB, four reference datasets...
The contribution of proliferation to B lymphocyte homeostasis and antigen responses is largely unknown. We quantified the replication history mouse human subsets by calculating ratio between genomic coding joints signal on kappa-deleting recombination excision circles (KREC) IGK-deleting rearrangement. This approach was validated with in vitro studies. demonstrate that naive mature lymphocytes, but not transitional undergo vivo homeostatic absence somatic mutations periphery. T...
The role of specific transcription factors in the initiation and regulation Ig gene rearrangements has been studied extensively mouse models, but data on normal human precursor B cell differentiation are limited. We purified five subsets, assessed quantified their IGH, IGK, IGL rearrangement patterns expression profiles. Pro-B cells already massively initiate D(H)-J(H) rearrangements, which completed with V(H)-DJ(H) pre-B-I cells. Large cycling pre-B-II selected for in-frame IGH...
Primary immunodeficiency disorders enable identification of genes with crucial roles in the human immune system. Here we study patients suffering from recurrent bacterial, viral and Cryptosporidium infections, identify a biallelic mutation MAP3K14 gene encoding NIK (NF-κB-inducing kinase). Loss kinase activity mutant NIK, predicted by silico analysis confirmed functional assays, leads to defective activation both canonical non-canonical NF-κB signalling. Patients mutated exhibit B-cell...
CD (cluster of differentiation) Ags are cell surface molecules expressed on leukocytes and other cells relevant for the immune system. nomenclature has been universally adopted by scientific community is officially approved International Union Immunological Societies sanctioned World Health Organization. It provides a unified designation system mAbs, as well that they recognize. This was established Human Leukocyte Differentiation Antigens Workshops. In addition to defining nomenclature,...
Abstract Measles causes a transient immune suppression, leading to increased susceptibility opportunistic infections. In experimentally infected non-human primates (NHPs) measles virus (MV) infects and depletes pre-existing memory lymphocytes, causing amnesia. A outbreak in the Dutch Orthodox Protestant community provided unique opportunity study pathogenesis of suppression unvaccinated children. peripheral blood mononuclear cells (PBMC) prodromal patients, we detected MV-infected CD4 + CD8...
The composition and function of the immune system in African children is linked to age, location, anemia.