Emily M. Mace
A5016163533- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunodeficiency and Autoimmune Disorders
- Immune cells in cancer
- CAR-T cell therapy research
- IL-33, ST2, and ILC Pathways
- Cell Adhesion Molecules Research
- Cytomegalovirus and herpesvirus research
- Immunotherapy and Immune Responses
- Reproductive System and Pregnancy
- Advanced Fluorescence Microscopy Techniques
- Lymphoma Diagnosis and Treatment
- Autoimmune and Inflammatory Disorders Research
- Parvovirus B19 Infection Studies
- Cancer Immunotherapy and Biomarkers
- DNA Repair Mechanisms
- HIV Research and Treatment
- Pneumocystis jirovecii pneumonia detection and treatment
- Tuberculosis Research and Epidemiology
- Peptidase Inhibition and Analysis
- Bladder and Urothelial Cancer Treatments
- Cellular Mechanics and Interactions
- Acute Myeloid Leukemia Research
- Advanced Electron Microscopy Techniques and Applications
- Hematopoietic Stem Cell Transplantation
Columbia University Irving Medical Center
2018-2025
Columbia University
2018-2025
Georgetown University Medical Center
2024
Georgetown University
2024
Baylor College of Medicine
2012-2023
Texas Children's Hospital
2013-2023
EIA University
2023
Morgan Stanley Children's Hospital
2019-2021
Royal College of Physicians
2020
Barnard College
2020
Accumulation of filamentous actin (F-actin) at the immunological synapse (IS) is a prerequisite for cytotoxic function natural killer (NK) cells. Subsequent to reorganization network, lytic granules polarize IS where their contents are secreted directly toward target cell, providing critical access host defense. There has been limited investigation into relationship between network and degranulation. Thus, we have evaluated secretion using microscopy techniques that provide unprecedented...
Abstract Human natural killer (NK) cells in peripheral blood perform many functions, and classification of specific subsets has been a longstanding goal. We report single-cell RNA sequencing NK cells, comparing gene expression unstimulated interleukin (IL)-2–activated from healthy cytomegalovirus (CMV)-negative donors. Three cell resembled well-described populations; CD56brightCD16−, CD56dimCD16+CD57−, CD56dimCD16+CD57+. CD56dimCD16+CD57− subdivided to include population with higher...
Hemophagocytic lymphohistiocytosis (HLH) is characterized by immune dysregulation due to inadequate restraint of overactivated cells and associated with a variable clinical spectrum having overlap more common pathophysiologies. HLH difficult diagnose can be part inflammatory syndromes. Here, we identify novel hematological/autoinflammatory condition (NOCARH syndrome) in four unrelated patients superimposable features, including neonatal-onset cytopenia dyshematopoiesis, autoinflammation,...
Primary immunodeficiency disorders enable identification of genes with crucial roles in the human immune system. Here we study patients suffering from recurrent bacterial, viral and Cryptosporidium infections, identify a biallelic mutation MAP3K14 gene encoding NIK (NF-κB-inducing kinase). Loss kinase activity mutant NIK, predicted by silico analysis confirmed functional assays, leads to defective activation both canonical non-canonical NF-κB signalling. Patients mutated exhibit B-cell...
Natural killer (NK) cells are innate immune effectors that lyse virally infected and tumorigenic through the formation of an immunological synapse. Actin remodeling at lytic synapse is a critical requirement for multiple facets cytotoxic function. Activating receptor integrin signaling leads to regulated turnover actin, which required adhesion, sustained signaling, ultimately exocytosis. NK undergo granule exocytosis in hypodense regions pervasive actin network. Although these requirements...
Lytic immune effector function depends upon directed secretion of cytolytic granules at the immunological synapse (IS) and requires dynamic rearrangement filamentous (F)-actin. Coronin 1A (Coro1A) is hematopoietic-specific member family actin regulators that promote F-actin disassembly. Here, we show Coro1A required for natural killer (NK) cell cytotoxic in two human NK lines ex vivo cells from a Coro1A-deficient patient. Using superresolution nanoscopy to probe IS, demonstrate promotes...
NK cell activation has been shown to be metabolically regulated in vitro; however, the role of metabolism during vivo responses infection is unknown. We examined glycolysis function murine cytomegalovirus (MCMV) and ability IL-15 prime cells CMV infection. The glucose inhibitor 2-deoxy-ᴅ-glucose (2DG) impaired both mouse human cytotoxicity following priming vitro. Similarly, MCMV-infected mice treated with 2DG had clearance NK-specific targets vivo, which was associated higher viral burden...