A5061015160- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Reproductive System and Pregnancy
- IL-33, ST2, and ILC Pathways
- Hematopoietic Stem Cell Transplantation
- Immune cells in cancer
- Lymphoma Diagnosis and Treatment
- Viral-associated cancers and disorders
- Cytomegalovirus and herpesvirus research
- Ovarian cancer diagnosis and treatment
- HIV Research and Treatment
- Chemokine receptors and signaling
- Immune Response and Inflammation
- Bladder and Urothelial Cancer Treatments
- Endometrial and Cervical Cancer Treatments
- SARS-CoV-2 and COVID-19 Research
- Protein Degradation and Inhibitors
- Autoimmune and Inflammatory Disorders Research
- Chronic Lymphocytic Leukemia Research
- COVID-19 and Mental Health
- Physiological and biochemical adaptations
- Oral Health Pathology and Treatment
Ospedale Policlinico San Martino
2021-2025
University of Genoa
2016-2025
German BioImaging – Gesellschaft für Mikroskopie und Bildanalyse
2020
Istituto Giannina Gaslini
2002-2005
National Institute of Allergy and Infectious Diseases
2003-2005
National Institutes of Health
2003-2005
University of Toronto
2005
Inserm
2002
Tecnologie Avanzate (Italy)
1998
Two major receptors involved in human natural cytotoxicity, NKp46 and NKp44, have recently been identified. However, experimental evidence suggested the existence of additional such receptor(s). In this study, by generation monoclonal antibodies (mAbs), we identified NKp30, a novel 30-kD triggering receptor selectively expressed all resting activated killer (NK) cells. Although mAb-mediated cross-linking NKp30 induces strong NK cell activation, masking inhibits cytotoxicity against normal or...
After culture in interleukin (IL)-2, natural killer (NK) cells acquire an increased capability of mediating non–major histocompatibility complex (MHC)–restricted tumor cell lysis. This may reflect, at least part, the de novo expression by NK triggering receptors involved cytolysis. In this study we identified a novel 44-kD surface molecule (NKp44) that is absent freshly isolated peripheral blood lymphocytes but progressively expressed all vitro after IL-2. Different from other markers...
The surface density of the triggering receptors responsible for natural killer (NK)-mediated cytotoxicity is crucial ability NK cells to kill susceptible target cells. In this study, we show that transforming growth factor β1 (TGFβ1) down-regulates expression NKp30 and in part NKG2D but not other such as NKp46. TGFβ1-mediated inhibition reflects gene regulation at transcriptional level. has been shown represent major receptor involved NK-mediated killing dendritic Accordingly,...
Natural killer (NK) cells are an important component of the innate immune response against viral infections. NK cell-mediated cytolytic activity is defective in HIV-infected individuals with high levels replication. In present study, we examined phenotypic and functional characteristics unusual CD56 – /CD16 + (CD56 ) subset that greatly expanded HIV-viremic individuals. The higher level expression inhibitory receptors lower natural cytotoxicity observed fraction compared was associated...
NKp46 is a novel triggering receptor expressed by all human NK cells that involved in natural cytotoxicity. In this study we show the surface density of may vary different and precise correlation exists between phenotype clones their cytotoxicity against HLA-class I-unprotected allogeneic or xenogeneic cells. Thus, NKp46bright efficiently lysed murine tumor while NKp46dull were poorly cytolytic both types target We also correlates with ability to lyse autologous Finally, was found be deeply...
Natural killer (NK) cells play a central role in host defense against various pathogens. Functional defects of NK HIV-1 infection as direct effect abnormal expression or function inhibitory receptors (iNKRs), activating natural cytotoxicity (NCRs), and NKG2D have not yet been described. This study demonstrates an expansion the functionally defective CD56 - /CD16 + population viremic versus aviremic patients. We also demonstrate that HIV-infected patients, iNKRs was well conserved most cases,...
Primary infection with the human oncogenic Epstein-Barr virus (EBV) can result in infectious mononucleosis (IM), a self-limiting disease caused by massive lymphocyte expansion that predisposes for development of distinct EBV-associated lymphomas. Why some individuals experience this symptomatic primary EBV infection, whereas majority acquires asymptomatically, remains unclear. Using mouse model reconstituted immune system components, we show depletion natural killer (NK) cells enhances IM...
Abstract Group 2 innate lymphoid cells (ILC2s) are involved in human diseases, such as allergy, atopic dermatitis and nasal polyposis, but their function cancer remains unclear. Here we show that, acute promyelocytic leukaemia (APL), ILC2s increased hyper-activated through the interaction of CRTH2 NKp30 with elevated tumour-derived PGD2 B7H6, respectively. ILC2s, turn, activate monocytic myeloid-derived suppressor (M-MDSCs) via IL-13 secretion. Upon treating APL all-trans retinoic acid...
Universal CAR T-cell therapies are poised to revolutionize cancer treatment and improve patient outcomes. However, realizing these advantages in an allogeneic setting requires universal T-cells that can kill target tumor cells, avoid depletion by the host immune system, proliferate without attacking tissues. Here, we describe development of a novel immune-evasive scaffold using precise TALEN-mediated gene editing DNA matrices vectorized recombinant adeno-associated virus 6. We simultaneously...
In humans, natural killer (NK) cell function is regulated by a series of receptors and coreceptors with either triggering or inhibitory activity. Here we describe novel 60-kD glycoprotein, termed NTB-A, that expressed all human NK, T, B lymphocytes. Monoclonal antibody (mAb)-mediated cross-linking NTB-A results in the induction NK-mediated cytotoxicity. Similar to 2B4 (CD244) functioning as coreceptor NK activation, also triggers cytolytic activity only cells expressing high surface...
Natural cytotoxicity receptors (NKp46, NKp44 and NKp30) play a predominant role in human NK cell triggering during natural cytotoxicity. Human 2B4 also induced activation redirected killing assays using anti-2B4 monoclonal antibodies (mAb) murine targets. Since this effect was confined to fraction of cells, suggested functional heterogeneity molecules. Here we show that via against targets is strictly dependent upon the engagement NKp46 by ligand (s) on target cells. Thus, clones expressing...
In this study we analyzed the progression of cell surface receptor expression during in vitro -induced human natural killer (NK) maturation from CD34 + Lin − precursors. NKp46 and NKp30, two major triggering receptors that play a central role cytotoxicity, were expressed before HLA class I-specific inhibitory receptors. Moreover, their appearance at correlated with acquisition cytolytic activity by developing NK cells. Although early may provide activating signals required for inducing...
In this study, we demonstrate that the in vitro interactions between a CD56(neg)/CD16(pos) (CD56(neg)) subset of natural killer (NK) cells and autologous dendritic (DCs) from HIV-1-infected viremic but not aviremic individuals are markedly impaired likely interfere with development an effective immune response. Among defective abnormalities process reciprocal NK-DC activation maturation as well defect NK cell-mediated editing or elimination immature DCs (iDCs). Notably, lysis mature (mDCs)...
In this study the phenotype and function of tumor-associated NK cells from peritoneal fluids a selected cohort patients with seropapillary ovarian carcinoma were analyzed. > 50% these patients, expression activating receptor NKp30 in was substantially reduced as compared to autologous peripheral blood (PB) cells. The impaired associated presence one its cellular ligands (B7-H6), which detectable surface/cytosolic molecule tumor soluble fluid. expressing NKp30low displayed an interferon-gamma...
Background: The HIV-1-induced expansion of highly dysfunctional natural killer (NK) cell subsets represents a strategy to evade NK antiviral functions. In this context, the loss NKG2Apos cells in chronic viremic HIV-1-infected individuals has also been associated with dramatic NKG2Cpos cells. viral trigger high frequencies expressing NKG2C is still being debated. Objective: To confirm that human cytomegalovirus (HCMV) infection necessary for and assess whether phenomenon affects NKG2A/NKG2C...