A5102755638- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Multiple Myeloma Research and Treatments
- IL-33, ST2, and ILC Pathways
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Protein Degradation and Inhibitors
- Eosinophilic Esophagitis
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Immune cells in cancer
- MicroRNA in disease regulation
- Peptidase Inhibition and Analysis
- Cytokine Signaling Pathways and Interactions
- FOXO transcription factor regulation
- Phagocytosis and Immune Regulation
- Cancer therapeutics and mechanisms
- Toxic Organic Pollutants Impact
- Toxin Mechanisms and Immunotoxins
- Extracellular vesicles in disease
- Phytochemistry and Bioactivity Studies
- Curcumin's Biomedical Applications
- Viral Infections and Immunology Research
- Bioactive Compounds and Antitumor Agents
- T-cell and Retrovirus Studies
The Ohio State University
2013-2023
The Ohio State University Comprehensive Cancer Center – Arthur G. James Cancer Hospital and Richard J. Solove Research Institute
2010-2021
Comprehensive Blood & Cancer Center
2018
Ohio University
2017
Cancer Genetics (United States)
2004
University of Pittsburgh
2003
NK cells are the dominant population of immune in endometrium secretory phase menstrual cycle and decidua early pregnancy. The possibility that this is a site cell development particular interest because cyclical death regeneration during cycle. To investigate this, we searched for developmental stages 1-4, based on expression CD34, CD117, CD94. In study, report heterogeneous stage 3 precursor (CD34(-)CD117(+)CD94(-)) mature 4 (CD34(-)CD117(-/+)CD94(+)) cells, but not multipotent 1 2...
Bioactive components from dietary supplements such as curcumin may represent attractive agents for cancer prevention or treatment. DNA methylation plays a critical role in acute myeloid leukemia (AML) development, and presents an excellent target treatment of this disease. However, it remains largely unknown how curcumin, component the popular Indian spice turmeric, hypomethylation to reactivate silenced tumor suppressor genes present potential option AML. Here we show that down-regulates...
Our goal is to test whether CS1 could be targeted by chimeric antigen receptor (CAR) T cells treat multiple myeloma (MM).We generated a retroviral construct of CS1-specific CAR and engineered primary human expressing the CAR. We then tested capacity CS1-CAR eradicate MM tumor in vitro, ex vivo, vivo using orthotopic xenograft mouse models.In compared with mock-transduced cells, upon recognizing CS1-positive CS1-CAR-transduced secreted more IFN-γ as well interleukin (IL)-2, expressed higher...
Abstract Purpose: Daratumumab and its use in combination with other agents is becoming a new standard of care for the treatment multiple myeloma. We mechanistically studied how daratumumab acts on natural killer (NK) cells. Experimental Design: Quantities NK cells peripheral blood and/or bone marrow patients myeloma or healthy donors were examined by flow cytometry. NK-cell apoptosis associated mechanism assessed cytometry immunoblotting. Patients' expanded vitro using feeder Combination was...
Human natural killer (NK) cells develop in secondary lymphoid tissues (SLTs) through distinct stages. We identified two SLT lineage (Lin)−CD34−CD117+/−CD94+CD16− "stage 4" subsets according to expression of the C-type lectin-like surface-activating receptor, NKp80: NKp80− (stage "4a") and NKp80+ "4b"). Whereas stage 4b expressed more transcription factors T-BET EOMES, produced interferon-gamma, were cytotoxic, 4a RORγt AHR interleukin-22, similar Lin−CD34−CD117+CD94−CD16− 3" cells, whose...
The metabolic checkpoint kinase mechanistic/mammalian target of rapamycin (mTOR) regulates natural killer (NK) cell development and function, but the exact underlying mechanisms remain unclear. Here, we show, via conditional deletion Raptor (mTORC1) or Rictor (mTORC2), that mTORC1 mTORC2 promote NK maturation in a cooperative non-redundant manner, mainly by controlling expression Tbx21 Eomes. Intriguingly, regulate cytolytic function an opposing way, exhibiting promoting inhibitory effects...
Accumulating evidence indicates that human natural killer (NK) cells develop in secondary lymphoid tissue (SLT) through a so-called "stage 3" developmental intermediate minimally characterized by CD34(-)CD117(+)CD94(-) immunophenotype lacks mature NK cell function. This stage 3 population is heterogeneous, potentially composed of functionally distinct innate (ILC) types include interleukin-1 receptor (IL-1R1)-positive, IL-22-producing ILC3s. Whether ILC3s are developmentally related to...
The development of a broad repertoire T cells, which is essential for effective immune function, occurs in the thymus. Although some data suggest that cell can occur extrathymically, many researchers remain skeptical extrathymic has an important role generating healthy individuals. However, it may be setting poor thymic function or congenital deficit and context autoimmunity, cancer, regenerative medicine. Here, we report evidence stepwise program within human tonsil. We identified 5...
Abstract Group 3 innate lymphoid cells (ILC3s) are important regulators of the immune system, maintaining homeostasis in presence commensal bacteria, but activating defenses response to microbial pathogens. ILC3s a robust source IL-22, cytokine critical for stimulating antimicrobial response. We sought identify cytokines that can promote proliferation and induce or maintain IL-22 production by determine molecular mechanism this process. identified IL-18 as cooperates with an ILC3 survival...
Autologous stem cell transplant (ASCT) is the standard of care for patients with multiple myeloma (MM). The clinical significance peripheral blood T lymphocyte (PBTL) immunologic changes associated ASCT poorly understood. Here we evaluated transcriptional messenger RNA profiles and immunophenotypes to correlate senescence, exhaustion, anergy endpoints in a cohort MM undergoing ASCT. induced global altered molecular levels markers subtypes, activation, exhaustion. These included reduced...
Natural products are a major source for cancer drug development. NK cells critical component of innate immunity with the capacity to destroy cells, cancer-initiating and clear viral infections. However, few reports describe natural product that stimulates cell IFN-γ production unravel mechanism action. In this study, through screening, we found product, phyllanthusmin C (PL-C), alone enhanced by human cells. PL-C also synergized IL-12, even at low cytokine concentration 0.1 ng/ml, stimulated...
Abstract Introduction: Ovarian cancer (OC) remains the most lethal gynecologic malignancy, with poor long-term survival, largely due to its diagnosis at advanced stages and high rate of recurrence resistance platinum therapies. This study evaluates therapeutic efficacy HO-3867, a STAT3 inhibitor, bevacizumab, an anti-VEGF antibody, alone in combination immunocompetent (IC) syngeneic ovarian progression model. Methods: The ability HO-3867 induce macrophage polarization was assessed RAW264.7...