A5061836023- Chronic Lymphocytic Leukemia Research
- Immunodeficiency and Autoimmune Disorders
- Lymphoma Diagnosis and Treatment
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- T-cell and Retrovirus Studies
- Blood disorders and treatments
- Reproductive System and Pregnancy
- Metastasis and carcinoma case studies
- Diabetes and associated disorders
- E-Government and Public Services
- Renal Diseases and Glomerulopathies
- Fungal Infections and Studies
- SARS-CoV-2 and COVID-19 Research
- Protein Degradation and Inhibitors
- Immunotherapy and Immune Responses
- Viral-associated cancers and disorders
- COVID-19 Clinical Research Studies
- Blood groups and transfusion
- Cancer Mechanisms and Therapy
- Mast cells and histamine
- Local Government Finance and Decentralization
- Complement system in diseases
Centro de Investigación del Cáncer
2014-2025
Universidad de Salamanca
2014-2025
Instituto de Salud Carlos III
2018-2025
Centro de Investigación Biomédica en Red de Cáncer
2018-2025
Instituto de Investigación Biomédica de Salamanca
2014-2025
University College London
2023-2024
Great Ormond Street Hospital
2023
Consejo Superior de Investigaciones Científicas
2021
Althaia
2021
Centro de Investigación Biomédica en Red
2019
<h3>Background</h3> Humoral immunocompetence develops stepwise throughout life and contributes to individual susceptibility infection, immunodeficiency, autoimmunity, neoplasia. Immunoglobulin heavy chain (IgH) isotype serum levels can partly explain such age-related differences, but their relationship with the IgH distribution within memory B-cell (MBC) plasma cell (PCs) compartments remains be investigated. <h3>Objective</h3> We studied of MBCs PCs expressing different isotypes in addition...
Abstract In the context of relapsed and refractory childhood pre-B cell acute lymphoblastic leukemia (R/R B-ALL), CD19-targeting chimeric antigen receptor (CAR)-T cells often induce durable remissions, which requires persistence CAR-T cells. this study, we systematically analyzed CD19 10 children with R/R B-ALL enrolled in CARPALL trial via high-throughput single-cell gene expression T sequencing infusion products serial blood bone marrow samples up to 5 years after infusion. We show that...
BackgroundPredominantly antibody deficiencies (PADs) are the most prevalent primary immunodeficiencies, but their B-cell defects and underlying genetic alterations remain largely unknown.ObjectiveWe investigated patients with PADs for distribution of 41 blood plasma cell (PC) subsets, including subsets defined by expression distinct immunoglobulin heavy chain subclasses.MethodsBlood samples from 139 PADs, 61 common variable immunodeficiency (CVID), 68 selective IgA deficiency (IgAdef), 10...
Background Chronic lymphocytic leukemia (CLL)-like monoclonal B lymphocytosis (MBL) with (MBLhi) or without (MBLlo) absolute B-lymphocytosis precedes most CLL cases,the specific determinants for malignant progression remaining unknown. Methodology/Principal Findings For this purpose, simultaneous iFISH and molecular analysis of well-established cytogenetic alterations chromosomes 11, 12, 13, 14 17 together the pattern rearrangement IGHV genes were performed in CLL-like cells from MBL cases....
Low-count monoclonal B-cell lymphocytosis is defined by the presence of very low numbers circulating clonal B cells, usually phenotypically similar to chronic lymphocytic leukemia whose biological and clinical significance remains elusive. Herein, we re-evaluated 65/91 low-count cases (54 leukemia-like 11 non-chronic leukemia-like) followed-up for a median seven years, using high-sensitivity flow cytometry interphase fluorescence in situ hybridization. Overall, clone size significantly...
Introduction In monoclonal B cell lymphocytosis (MBL) and chronic lymphocytic leukemia (CLL), the expansion of malignant cells disrupts normal homeostasis interactions between T cells, leading to immune dysregulation. CD20+ are a subpopulation that appear be involved in autoimmune diseases cancer. Methods Here, we quantified phenotypically characterized from MBL subjects CLL patients using flow cytometry correlated our findings with B-cell receptor mutational status other features disease....
The early immune kinetics after SARS-CoV-2 vaccination remain poorly understood, particularly among individuals with low-count monoclonal B-cell lymphocytosis (MBLlo). We investigated the cellular and humoral in blood of 50 non-MBL healthy donors (HD) vs. 16 MBLlo subjects vaccination, who were subclassified according to their history previous exposure into naïve previously infected subjects. Overall, we found decreased neutrophil lymphocyte counts at day +4 following each dose HD, together...
Background/Objectives: Despite the current international prognostic index for chronic lymphocytic leukemia (CLL) being widely accepted and broadly used, it does not consider kinetics of B-cell clone over time. Here, we investigated potential association between distinct features leukemic cells other immune in blood clonal B-cells CLL stage Binet A/Rai 0 (A/0) patients; Methods: Based on cell kinetics, 69 A/0 cases followed a median 105 months were classified as carrying stable (n = 53) vs....
Chronic lymphocytic leukemia (CLL) is the most common adult in Western countries, which characterized by accumulation of mature CD5 + /CD20 lo /CD23 clonal B-cells peripheral blood (PB), bone marrow (BM), and other lymphoid tissues [1].Currently, it well-established that CLL systematically preceded a pre-leukemic stage, known as monoclonal B-cell lymphocytosis (MBL) [2]; MBL includes both low-count (MBL ) high-count hi ), depending on number PB Bcells (<0.5 × 10 9 /L ≥0.5 /L, respectively) detected
Chronic antigen-stimulation has been recurrently involved in the earlier stages of monoclonal B-cell lymphocytosis, chronic lymphocytic leukemia and other lymphoproliferative disorders. The expansion two or more clones frequently reported individuals with these conditions; potentially, such coexisting have a greater probability interaction common immunological determinants. Here, we analyzed receptor repertoire molecular profile, as well phenotypic, cytogenetic hematologic features, 228...
IIdiopathic T-CD4 lymphocytopenia (ICL) is a rare heterogeneous syndrome characterized by opportunistic infections due to reduced CD4 T lymphocytes (<300 cells/uL or <20% of cells) in the absence HIV infection and other primary causes lymphopenia. Molecular testing ICL has revealed defects genes not specific T-cells, with pleiotropic effects on cell types. Here we report for first time an absolute (<0.01 CD4+ T-cells/µL) autosomic recessive gene mutation that completely abrogates protein...
Reference ranges of blood-circulating leukocyte populations by, e.g., age and sex, are required for monitoring immune-cell kinetics. Most previous reports in which flow cytometry has been used to define the reference counts included a limited number donors and/or cell did not consider sex simultaneously. Moreover, other factors previously considered definition normal ranges, such as presence chronic-lymphocytic-leukemia (CLL)-like low-count monoclonal B-cell lymphocytosis (MBLlo), might also...
Patients diagnosed with chronic lymphocytic leukemia (CLL) display a high incidence of infections due to an associated immunodeficiency that includes hypogammaglobulinemia. A higher risk has also been recently reported for high-count monoclonal B-cell lymphocytosis, while no information is available in low-count lymphocytosis. Here, we evaluated the status humoral immune system patients (n=58), as well low- (n=71) and high- (n=29) count lymphocytosis versus healthy donors (n=91). Total free...
A wide variety of immunoglobulins (Ig) is produced by the immune system thanks to different mechanisms (V(D)J recombination, somatic hypermutation, and antigen selection). The profiling Ig sequences (at both DNA peptide levels) are great relevance developing targeted vaccines or treatments for specific diseases infections. Thus, genomics proteomics techniques (such as Next-Generation Sequencing (NGS) mass spectrometry (MS)) have notably increased knowledge in sequencing serum a...
Summary An increasing body of evidence suggests the potential occurrence antigen encounter by cell origin in chronic lymphocytic leukaemia ( CLL ) and ‐like monoclonal B ‐cell lymphocytosis MBL ). However, scenario which this event might occur remains unknown. In order to gain insight into we investigated molecular, cytogenetic haematological features 223 n = 84) 139) clones with stereotyped 32) versus non‐stereotyped 191) immunoglobulin heavy chain variable region IGHV amino acid sequences....
Studies about the social impact of Internet and information communication technologies (ICTs) are usually focused on private sector organizations. However, under rubric e-government, developments in public proceeding at a similar if not greater pace giving rise to range important issues which internal (organisational managerial) external (relations between government citizens clients) dimensions management administration. Despite importance these developments, there is dearth academic...
Abstract Chimeric antigen receptor (CAR)-modified T-cells have become established as an effective treatment of haematological cancers. In the context relapsed and refractory childhood pre-B cell acute lymphoblastic leukaemia (B ALL), CD19 targeting CAR often induce durable remissions. One most critical determinants achieving a response is persistence T-cells. Here, we systematically analysed T cells ten children with or B ALL enrolled in CARPALL trial (NCT02443831). We performed high...
The HCDR3 sequences of the B-cell receptor (BCR) undergo constraints in length, amino acid use, and charge during maturation precursors after antigen encounter, leading to BCR antibodies with high affinity specific antigens. Chronic lymphocytic leukemia consists an expansion B-cells a mixed immature “antigen-experienced” phenotype, either mutated (M-CLL) or unmutated (U-CLL) tumor BCR, associated distinct patient outcomes. Here, we investigated hydropathy index 138 CLL patients its...