A5038639464- Advanced Proteomics Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Chronic Lymphocytic Leukemia Research
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Immune Cell Function and Interaction
- Environmental and Ecological Studies
- Single-cell and spatial transcriptomics
- Gene expression and cancer classification
- Immune cells in cancer
- Biosensors and Analytical Detection
- Plant-derived Lignans Synthesis and Bioactivity
- RNA and protein synthesis mechanisms
- Phytochemical compounds biological activities
- Vanadium and Halogenation Chemistry
- Nanofabrication and Lithography Techniques
- Ubiquitin and proteasome pathways
- Mass Spectrometry Techniques and Applications
- Regional Development and Innovation
- Nanoparticles: synthesis and applications
- Metal-Catalyzed Oxygenation Mechanisms
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Lymphoma Diagnosis and Treatment
Universidad de Salamanca
2012-2025
Centro de Investigación del Cáncer
2015-2025
Instituto de Investigación Biomédica de Salamanca
2015-2025
Centro de Investigación Biomédica en Red de Cáncer
2020-2025
Instituto de Salud Carlos III
2025
Central University Hospital of Asturias
2023-2024
Universidad de Oviedo
2023-2024
Leiden University Medical Center
2021-2024
Gobierno del Principado de Asturias
2024
Instituto de Investigación Sanitaria del Principado de Asturias
2023
Ovarian cancer is one of the deadliest cancers in women. The lack specific symptoms, especially at initial stages disease development, together with malignancy heterogeneity, lower life expectancy patients. Aiming to improve survival rates, diagnostic and prognostic biomarkers are increasingly employed clinics, providing gynecologists oncologists new tools guide their treatment decisions. Despite vast number investigations, there still an urgent need discover more ovarian subtype-specific...
Defective FAS (CD95/Apo-1/TNFRSF6) signaling causes autoimmune lymphoproliferative syndrome (ALPS). Hypergammaglobulinemia is a common feature in ALPS with mutations (ALPS-FAS), but paradoxically, fewer conventional memory cells differentiate from FAS-expressing germinal center (GC) B cells. Resistance to FAS-induced apoptosis does not explain this phenotype. We tested the hypothesis that defective non-apoptotic may contribute impaired cell differentiation ALPS. analyzed secondary lymphoid...
This paper summarizes the recent activities of Chromosome-Centric Human Proteome Project (C-HPP) consortium, which develops new technologies to identify yet-to-be annotated proteins (termed "missing proteins") in biological samples that lack sufficient experimental evidence at protein level for confident identification. The C-HPP also aims forms may be caused by genetic variability, post-translational modifications, and alternative splicing. Proteogenomic data integration basis C-HPP's...
Vanadium complexes were studied during recent years and considered as a representative of new class non-platinum metal antitumor agents in combination with their low toxicity. However, few challenges still remain the discovery molecular targets for these novel metal-based drugs. The study cell signaling pathways related to vanadium drugs, which is highly critical identifying specific that play an important role activity compounds, scarce. This research deals alterations intracellular...
The immunocompability of polyelectrolyte capsules synthesized by layer-by-layer deposition has been investigated. Capsules different architecture and composed either non-degradable or biodegradable polymers, with positively negatively charged outer surface, micrometer size, have used, the capsule uptake cell lines studied quantified. Immunocompatibility studies were performed peripheral blood mononuclear cells (PBMCs). Data demonstrate that incubation capsules, at concentrations relevant for...
Introduction Monitoring of innate myeloid cells (IMC) is broadly applied in basic and translational research, as well diagnostic patient care. Due to their immunophenotypic heterogeneity biological plasticity, analysis IMC populations typically requires large panels markers. Currently, two cytometry-based techniques allow for the simultaneous detection ≥40 markers: spectral flow cytometry (SFC) mass (MC). However, little known about comparability SFC MC studying populations. Methods We...
B-cell chronic lymphocytic leukemia (B-CLL) is characterized by highly heterogeneous genomic alterations and altered signaling pathways, with limited studies on its proteome. Our study presents a comprehensive analysis of the proteome phosphoproteome in B-CLL CLL-like monoclonal lymphocytosis (MBL) primary cells. Using high-resolution mass spectrometry, we identified 2970 proteins 316 phosphoproteins across five tumor samples, including 55 newly phosphopeptides (ProteomeXchange-PXD005997)....
Vanadium compounds were studied in recent years by considering them as a representative of new class non-platinum metal anticancer drugs.
Nowadays, massive genomics and transcriptomics data can be generated at the single-cell level. However, proteomics in this setting is still a big challenge. Despite great improvements sensitivity performance of mass spectrometry instruments better knowledge on sample preparation processing, it widely acknowledged that multistep workflows may lead to substantial loss, especially when working with paucicellular samples. Still, clinical fields, frequently limited amounts are available for...
Osteoarthritis (OA) is one of the most prevalent articular diseases. The identification proteins closely associated with diagnosis, progression, prognosis, and treatment response dramatically required for this pathology. In work, differential serum protein profiles have been identified in OA rheumatoid arthritis (RA) by antibody arrays containing 151 antibodies against 121 antigens a cohort 36 samples. Then validated larger 282 overall immunoreactivity higher pathological situations...
A comprehensive study of the molecular active landscape human cells can be undertaken to integrate two different but complementary perspectives: transcriptomics, and proteomics. After genome era, proteomics has emerged as a powerful tool simultaneously identify characterize compendium thousands proteins in cell. Thus, Chromosome-centric Human Proteome Project (C-HPP) is promoting full characterization proteome combining high-throughput with data derived from genome-wide expression profiling...
A first research development progress report of the Chromosome 19 Consortium with members from Sweden, Norway, Spain, United States, China and India, a part Chromosome-centric Human Proteome Project (C-HPP) global initiative, is presented (http://www.c-hpp.org). From chromosome peptide-targeted library constituting 6159 peptides, pilot study was conducted using subset 125 isotope-labeled peptides. We applied an annotation strategy triple quadrupole, ESI-Qtrap, MALDI mass spectrometry...
Chronic lymphocytic leukaemia (CLL) is a malignant B cell disorder characterized by its high heterogeneity. Although genomic alterations have been broadly reported, protein studies are still in their early stages. Herein, 224‐antibody microarray has employed to study the intracellular signalling pathways cohort of 14 newly diagnosed B‐CLL patients as preliminary for further investigations. Several profiles were differentially identified across cytogenetic and molecular presented samples...
Innate myeloid cell (IMC) populations form an essential part of innate immunity. Flow cytometric (FCM) monitoring IMCs in peripheral blood (PB) has great clinical potential for disease due to their role maintenance tissue homeostasis and ability sense micro-environmental changes, such as inflammatory processes damage. However, the lack standardized validated approaches hampered broad implementation. For accurate identification separation IMC populations, 62 antibodies against 44 different...
Monocytes are bone marrow-derived leukocytes that part of the innate immune system. divided into three subsets: classical, intermediate and non-classical, which can be differentiated by their expression some surface antigens, mainly CD14 CD16. These cells key players in inflammation process underlying mechanism many diseases. Thus, molecular characterization these may provide very useful information for understanding biology health disease. We performed a multicentric proteomic study with...
Approximately 18% of all human genes purported to encode proteins have not been directly evidenced at the protein level, according validation criteria established by neXtProt, and are considered be "missing" proteins. One goals Chromosome-Centric Human Proteome Project (C-HPP) is identify as many these missing possible in samples using mass spectrometry-based methods. To further this goal, a consortium C-HPP teams (chromosomes 5, 10, 16, 19) has joined forces devise new strategies use...