A5053411065- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Electrostatics and Colloid Interactions
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Innovative Microfluidic and Catalytic Techniques Innovation
- Nanopore and Nanochannel Transport Studies
- Electrochemical sensors and biosensors
- 3D Printing in Biomedical Research
- Silicon Nanostructures and Photoluminescence
- Extracellular vesicles in disease
- Electrical and Bioimpedance Tomography
- Analytical Chemistry and Sensors
- Neuroscience and Neural Engineering
- Electrohydrodynamics and Fluid Dynamics
- Nanofabrication and Lithography Techniques
- Ion-surface interactions and analysis
- Electrowetting and Microfluidic Technologies
- Nanowire Synthesis and Applications
- Analytical Chemistry and Chromatography
- Microbial Inactivation Methods
- Molecular Biology Techniques and Applications
Lund University
2016-2025
Dongguk University
2010-2017
Halo Labs (United States)
2017
The University of Tokyo
2017
Government of the Republic of Korea
2015
Institute for Biomedicine
2014
Medicon Village
2013
Memorial Sloan Kettering Cancer Center
2009-2012
Sungkyunkwan University
2010
University of Virginia
2009
A novel method, free flow acoustophoresis (FFA), capable of continuous separation mixed particle suspensions into multiple outlet fractions is presented. Acoustic forces are utilized to separate particles based on their size and density. The method shown be suitable for both biological nonbiological suspended particles. microfluidic chips were fabricated using conventional microfabrication methods. Particle was accomplished by combining laminar with the axial acoustic primary radiation force...
Circulating tumor cells (CTC) are shed in peripheral blood at advanced metastatic stages of solid cancers. Surface-marker-based detection CTC predicts recurrence and survival colorectal, breast, prostate cancer. However, scarcity variation size, morphology, expression profile, antigen exposure impairs reliable characterization CTC. We have developed a noncontact, label-free microfluidic acoustophoresis method to separate cancer from white (WBC) through forces generated by ultrasonic...
This acoustofluidics tutorial focuses on continuous flow-based half wavelength resonator systems operated in the transversal mode, where direction of primary acoustic force acts plane with microchip. The actuation mode facilitates integration up- and downstream microchannel networks as well visual control focusing experiment. Applications particle enrichment an are discussed clarification carrier fluid from undesired particles. Binary separation particle/vesicle/cell mixtures into two...
A new method is reported on how to measure the local pressure amplitude and Q factor of ultrasound resonances in microfluidic chips designed for acoustophoresis particle suspensions. The relies tracking individual polystyrene tracer microbeads straight water-filled silicon/glass microchannels. system actuated by a PZT piezo transducer attached beneath chip driven an applied ac voltage near its eigenfrequency 2 MHz. For given frequency number tracks are recorded CCD camera fitted theoretical...
We present microparticle image velocimetry measurements of suspended microparticles diameters from $0.6$ to $10\phantom{\rule{0.28em}{0ex}}\ensuremath{\mu}\text{m}$ undergoing acoustophoresis in an ultrasound symmetry plane a microchannel. The motion the smallest particles is dominated by Stokes drag induced acoustic streaming flow, while largest radiation force. For all particle sizes we predict theoretically how much velocity due and streaming, respectively. These predictions include...
A method to separate suspended particles from their medium in a continuous mode at microchip level is described. The combines an ultrasonic standing wave field with the extreme laminar flow properties obtained silicon micro channel. channel was 750 microm wide and 250 deep vertical side walls defined by anisotropic wet etching. suspension comprised "Orgasol 5 microm" polyamide spheres distilled water. perfused applying under pressure (suction) outlets. ultrasonically actuated back of chip...
Improved continuous acoustic particle separation (separation efficiency close to 100%) and of erythrocytes (red blood cells) from lipid microemboli in whole is reported.
Silicon microchips with immobilized antibodies were used to develop microfluidic enzyme immunoassays using chemiluminescence detection and horseradish peroxidase (HRP) as the label. Polyclonal anti-atrazine coupled silicon microchip surface an overall dimension of 13.1 × 3.2 mm, comprising 42 porous flow channels 235-μm depth 25-μm width. Different immobilization protocols based on covalent or noncovalent modification silica 3-aminopropyltriethoxysilane (APTES)...
A method to continuously separate different particle types in a suspension is reported. Acoustic forces standing wave field were utilized discriminate lipid particles from erythrocytes whole blood. The presented technology proposes new of cleaning, i.e. removing emboli from, shed blood recovered during cardiac surgery. Blood contaminated with enter laminar flow micro channel. Erythrocytes and suspended plasma are exposed half wavelength orthogonal the direction as they pass through Because...
Techniques for manipulating, separating, and trapping particles cells are highly desired in today's bioanalytical biomedical field. The microfluidic chip-based acoustic noncontact method earlier developed within the group now provides a flexible platform performing cell- particle-based assays continuous flow microsystems. An standing wave is generated etched glass channels (600 × 61 μm2) by miniature ultrasonic transducers (550 550 200 μm3). Particles or passing transducer will be retained...
Protein identification through peptide mass mapping by matrix-assisted laser desorption/ionization time-of-flight spectrometry (MALDI-TOF MS) has become a standard technique, used in many laboratories around the world. The traditional methodology often includes long incubations (6−24 h) and extensive manual steps. In an effort to address this, integrated microanalytical platform been developed for automated of proteins. silicon micromachined analytical tools, i.e., microchip immobilized...
The generation of high quality plasma from whole blood is major interest for many biomedical analyses and clinical diagnostic methods. However, it has proven to be a challenge make use microfluidic separation devices process fluids with cell content, such as blood. Here, we report on an acoustophoresis based chip that prepares linked application. This acoustic separator the capacity sequentially remove enriched cells in multiple steps yield low cellular content. generated fulfills standard...
Acoustic trapping of sub-micron particles can allow enrichment and purification small-sized low-abundance microorganisms. In this paper, we investigate the dependency ability to capture on particle concentration. Based findings, it is demonstrated that seed be introduced acoustic trapping, enable particles. Without using particles, continuous 490 nm polystyrene in a rectangular capillary with locally generated field at high concentrations, i.e. above 1% wt. Trapping significantly lower...
We derive analytical expressions for the three-dimensional (3D) acoustophoretic motion of spherical microparticles in rectangular microchannels. The is generated by acoustic radiation force and streaming-induced drag force. In contrast to classical theory Rayleigh streaming shallow, infinite, parallel-plate channels, our does include effect microchannel side walls. resulting predictions agree well with numerics experimental measurements polystyrene spheres nominal diameters 0.537 5.33 μm. 3D...
We present a platform for micro particle image velocimetry (μPIV), capable of carrying out full-channel, temperature-controlled, long-term-stable, and automated μPIV-measurement microchannel acoustophoresis with uncertainties below 5% spatial resolution in the order 20 μm. A method to determine optimal μPIV-settings obtaining high-quality results spatially inhomogeneous acoustophoretic velocity fields large dynamical range is presented. In particular we study dependence on μPIV interrogation...
Non-contact trapping using acoustic standing waves has shown promising results in cell-based research lately. However, the devices demonstrated are normally fabricated microfabrication or precision machining methods leading to a high unit cost. In e.g. clinical forensic applications avoiding cross-contamination, carryover infection is of outmost importance. these disposable key elements, thus making cost per critical factor. A solution presented here where low-cost off-the-shelf glass...
Enrichment of rare cells from peripheral blood has emerged as a means to enable noninvasive diagnostics and development personalized drugs, commonly associated with prerequisite concentrate the enriched cell population prior molecular analysis or culture. However, common concentration by centrifugation important limitations when processing low numbers. Here, we report on an integrated acoustophoresis-based enrichment system combined concentration. Polystyrene 7 μm microparticles could be...
Handling of sub-micrometer bioparticles <italic>e.g.</italic> bacteria is becoming increasingly important in the biomedical field and environmental food analysis.