A5066812910- Immune cells in cancer
- Advanced Proteomics Techniques and Applications
- Single-cell and spatial transcriptomics
- Immune Cell Function and Interaction
- Mass Spectrometry Techniques and Applications
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Metabolomics and Mass Spectrometry Studies
Leiden University Medical Center
2021-2023
Introduction Monitoring of innate myeloid cells (IMC) is broadly applied in basic and translational research, as well diagnostic patient care. Due to their immunophenotypic heterogeneity biological plasticity, analysis IMC populations typically requires large panels markers. Currently, two cytometry-based techniques allow for the simultaneous detection ≥40 markers: spectral flow cytometry (SFC) mass (MC). However, little known about comparability SFC MC studying populations. Methods We...
Nowadays, massive genomics and transcriptomics data can be generated at the single-cell level. However, proteomics in this setting is still a big challenge. Despite great improvements sensitivity performance of mass spectrometry instruments better knowledge on sample preparation processing, it widely acknowledged that multistep workflows may lead to substantial loss, especially when working with paucicellular samples. Still, clinical fields, frequently limited amounts are available for...
Innate myeloid cell (IMC) populations form an essential part of innate immunity. Flow cytometric (FCM) monitoring IMCs in peripheral blood (PB) has great clinical potential for disease due to their role maintenance tissue homeostasis and ability sense micro-environmental changes, such as inflammatory processes damage. However, the lack standardized validated approaches hampered broad implementation. For accurate identification separation IMC populations, 62 antibodies against 44 different...
Mass spectrometry (MS)-based proteomics profiling has undoubtedly increased the knowledge about cellular processes and functions. However, its applicability for paucicellular sample analyses is currently limited. Although new approaches have been developed single-cell studies, most of them not (yet) standardized and/or require highly specific (often home-built) devices, thereby limiting their broad implementation, particularly in non-specialized settings. To select an optimal MS-oriented...