A5046034812- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Diabetes and associated disorders
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- RNA and protein synthesis mechanisms
- Immunotherapy and Immune Responses
- Pancreatic function and diabetes
- Biosensors and Analytical Detection
- Epigenetics and DNA Methylation
- vaccines and immunoinformatics approaches
- Cardiac electrophysiology and arrhythmias
- Fibroblast Growth Factor Research
- Ion channel regulation and function
- Tryptophan and brain disorders
- Diabetes Management and Research
- Acute Myeloid Leukemia Research
- Analytical chemistry methods development
- T-cell and B-cell Immunology
- Peptidase Inhibition and Analysis
- Receptor Mechanisms and Signaling
- Galectins and Cancer Biology
- Genomics and Phylogenetic Studies
- Escherichia coli research studies
Washington University in St. Louis
2013-2025
The University of Texas Medical Branch at Galveston
2012-2019
Glenville State College
2017
Virginia Commonwealth University
2017
Texas A&M University at Galveston
2013
Université Nantes Angers Le Mans
2012
Centre National de la Recherche Scientifique
2012
Nantes Université
2012
Inserm
2012
University of Arkansas for Medical Sciences
2005-2010
The genetic alterations responsible for an adverse outcome in most patients with acute myeloid leukemia (AML) are unknown.Using massively parallel DNA sequencing, we identified a somatic mutation DNMT3A, encoding methyltransferase, the genome of cells from patient AML normal karyotype. We sequenced exons DNMT3A 280 additional de novo to define recurring mutations.A total 62 281 (22.1%) had mutations that were predicted affect translation. 18 different missense mutations, common which was...
Tetrahydrocannabinol (Δ<sup>9</sup>-THC), the primary psychoactive ingredient in marijuana, is subject to cytochrome P450 oxidation and subsequent UDP-glucuronosyltransferase (UGT)-dependent glucuronidation. Many studies have shown that CYP2C9 CYP3A4 are enzymes responsible for these P450-dependent oxidations, but little work has been done characterize phase II metabolic pathways. In this study, we test hypothesis there specific human UGTs classic cannabinoid metabolism. The activities of 12...
The pancreatic islet microenvironment is highly oxidative, rendering β cells vulnerable to autoinflammatory insults. Here, we examined the role of resident macrophages in autoimmune attack that initiates type 1 diabetes. Islet expressed CXCL16, a chemokine and scavenger receptor for oxidized low-density lipoproteins (OxLDLs), regardless predisposition. Deletion Cxcl16 nonobese diabetic (NOD) mice suppressed development Mechanistically, deficiency impaired clearance OxLDL by macrophages,...
The Haemophilus influenzae HMW1 adhesin is a high-molecular weight protein that secreted by the bacterial two-partner secretion pathway and mediates adherence to respiratory epithelium, an essential early step in pathogenesis of H. disease. In recent work, we discovered glycoprotein undergoes N-linked glycosylation at multiple asparagine residues with simple hexose units rather than N-acetylated units, revealing unusual N-glycosidic linkage suggesting new glycosyltransferase activity....
Summary Caenorhabditis elegans expresses a glutathione transferase (GST) belonging to the Pi class, for which we propose name CeGSTP2‐2. CeGSTP2‐2 (the product of gst‐10 gene) has ability conjugate lipid peroxidation 4‐hydroxynonenal (4‐HNE). Transgenic C. strains were generated in 5′‐flanking region and promoter placed upstream mGsta4 cDNAs, respectively. encodes murine mGSTA4‐4, an enzyme with particularly high catalytic efficiency 4‐HNE. The localization both transgenes was similar that...
The endogenous signaling molecule S-nitrosoglutathione (GSNO) and other S-nitrosylating agents can cause full maturation of the abnormal gene product ΔF508 cystic fibrosis (CF) transmembrane conductance regulator (CFTR). However, molecular mechanism action is not known. Here we show that Hsp70/Hsp90 organizing protein (Hop) a critical target GSNO, its S-nitrosylation results in CFTR cell surface expression. by GSNO inhibited association Hop with endoplasmic reticulum. This effect was...
Voltage-gated sodium channels (Nav1.1-Nav1.9) are responsible for the initiation and propagation of action potentials in neurons, controlling firing patterns, synaptic transmission plasticity brain circuit. Yet, it is protein-protein interactions macromolecular complex that exert diverse modulatory actions on channel, dictating its ultimate functional outcome. Despite fundamental role Nav brain, information proteome still lacking. Here we used affinity purification from crude membrane...
This paper summarizes the recent activities of Chromosome-Centric Human Proteome Project (C-HPP) consortium, which develops new technologies to identify yet-to-be annotated proteins (termed "missing proteins") in biological samples that lack sufficient experimental evidence at protein level for confident identification. The C-HPP also aims forms may be caused by genetic variability, post-translational modifications, and alternative splicing. Proteogenomic data integration basis C-HPP's...
The lymphatic fluid is the conduit by which part of tissue "omics" transported to draining lymph node for immunosurveillance. Following cannulation pre-nodal cervical and mesenteric afferent lymphatics, herein we investigate proteomic composition, uncovering that its composition varies according origin. Tissue specificity also reflected in dendritic cell-major histocompatibility complex class II-eluted immunopeptidome harvested from nodes. inflammatory disruption gut barrier, antigenic loads...
Resilience and vulnerability to neuropsychiatric disorders are linked molecular changes underlying excitability that still poorly understood. Here, we identify glycogen-synthase kinase 3β (GSK3β) voltage-gated Na+ channel Nav1.6 as regulators of neuroplasticity induced by environmentally enriched (EC) or isolated (IC) conditions-models for resilience vulnerability. Transcriptomic studies in the nucleus accumbens from EC IC rats predicted low levels GSK3β SCN8A mRNA a protective phenotype...
Cardiac voltage-gated Na+ (Nav) channels are key determinants of action potential waveforms, refractoriness and propagation, Nav1.5 is the main Nav pore-forming (α) subunit in mammalian heart. Although direct phosphorylation protein has been suggested to modulate various aspects channel physiology pathophysiology, native sites have not identified. In experiments here, a mass spectrometry (MS)-based proteomic approach was developed identify directly. Using an anti-NavPAN antibody, complexes...
Glioblastoma (GBM) is the most common adult primary brain tumor. Despite aggressive multimodal therapy, survival of patients with GBM remains dismal. However, recent evidence has demonstrated promise bone marrow-derived mesenchymal stem cells (BM-hMSCs) as a therapeutic delivery vehicle for anti-glioma agents due to their ability migrate or home human gliomas. While several studies have feasibility harnessing homing capacity BM-hMSCs targeted cancer therapeutics, it now also evident, based...