A5001063223- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- Cell Adhesion Molecules Research
- Immunotherapy and Immune Responses
- Complement system in diseases
- HIV Research and Treatment
- Malaria Research and Control
- COVID-19 Clinical Research Studies
- Immunodeficiency and Autoimmune Disorders
- Immune Response and Inflammation
- Protein purification and stability
- Influenza Virus Research Studies
- Galectins and Cancer Biology
- Platelet Disorders and Treatments
- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Mast cells and histamine
- Asthma and respiratory diseases
- Receptor Mechanisms and Signaling
- Diabetes and associated disorders
- Respiratory viral infections research
- HER2/EGFR in Cancer Research
The University of Melbourne
2016-2025
Burnet Institute
2016-2025
Monash University
2016-2025
Alfred Health
2020-2021
Seattle University
2021
Box Hill Hospital
2020
Eastern Health
2020
Melbourne Clinic
2020
Melbourne Sexual Health Centre
2020
The University of Sydney
2018
Memory B cells specific for SARS-CoV-2 spike and nucleocapsid proteins persist in peripheral blood after recovery from COVID-19.
Abstract The hallmarks of COVID-19 are higher pathogenicity and mortality in the elderly compared to children. Examining baseline SARS-CoV-2 cross-reactive immunological responses, induced by circulating human coronaviruses (hCoVs), is needed understand such divergent clinical outcomes. Here we show analysis coronavirus antibody responses pre-pandemic healthy children ( n = 89), adults 98), 57), patients 50) systems serology. Moderate levels cross-reactive, but non-neutralizing, antibodies...
The Ly phenotype of cells mediating skin graft rejection was determined using monoclonal anti-Lyt-1.1 and Lyt-2.1 antibodies in CBA mice that received lymphoid from sensitized to C57BL/6; i.e., alloantigenic differences arising the H-2 non-H-2 loci. It clear due wholly presence Lyt-1 inoculum Lyt-123 or Lyt-23 had no effect. Furthermore, synergism noted between Lyt-2 cells. In this model, both cytotoxic T cell lymphocyte precursors were shown be these could depleted populations mediated...
Abstract The interaction of Abs with their specific FcRs is primary importance in host immune effector systems involved infection and inflammation, are the target for evasion by pathogens. FcγRIIa a unique most widespread activating FcR humans that through avid binding complexes potently triggers inflammation. Polymorphisms (high responder/low responder [HR/LR]) linked to susceptibility infections, autoimmune diseases, efficacy therapeutic Abs. In this article, we define three-dimensional...
Ab-dependent cellular cytotoxicity, phagocytosis, and Ag presentation are key mechanisms of action Abs arising in vaccine or naturally acquired immunity, as well therapeutic mAbs. Cells expressing the low-affinity FcγRs (FcγRII CD32 FcγRIII CD16) activated for these functions when receptors aggregated following binding IgG-opsonized targets. Despite diversity Fc receptor proteins, IgG ligands, potential responding cell types, induction all FcγR-mediated responses by opsonized targets...
Abstract A highly protective vaccine will greatly facilitate achieving and sustaining malaria elimination. Understanding mechanisms of antibody-mediated immunity is crucial for developing vaccines with high efficacy. Here, we identify key roles in humoral Fcγ-receptor (FcγR) interactions opsonic phagocytosis sporozoites. We a major role neutrophils mediating phagocytic clearance sporozoites peripheral blood, whereas monocytes contribute minor role. Antibodies also promote natural killer cell...
The CH2-CH3 interface of the IgG Fc domain contains binding sites for a number receptors including Staphylococcal protein A and neonatal receptor (FcRn). It has recently been proposed that also principal site an isoform low affinity II (Fc gamma RIIb). RI RII have previously mapped to lower hinge adjacent surface CH2 although contributions suggested. This study addresses question whether plays role in interaction with RIIa. We demonstrate recombinant soluble murine human RIIa did not compete...
Human IgG Fc receptor (Fc gamma R) cDNA clones were isolated by cross-species hybridization probing libraries with the low-affinity R beta 1 clone from mouse as well a pool of oligonucleotides constructed nucleotide sequence this R. Three and analysis predicted amino acid indicated that human protein is synthesized 34-amino leader mature composed 281 acids. The extracellular region was divided into two domains, which very similar to each other corresponding regions both alpha Rs showed clear...
Immune complex (IC)-induced inflammation is integral to the pathogenesis of several autoimmune diseases. ICs activate complement system and interact with IgG FcgammaR. In this study, we demonstrate that activation system, specifically generation C5a, initiates neutrophilic in IC peritonitis. We show ablation C5a receptor signaling abrogates neutrophil recruitment wild-type mice prevents enhancement migration seen FcgammaRIIB(-/-) mice, suggesting C5aR crucial initial event upstream FcgammaR...
Infection by Staphylococcus aureus can result in severe conditions such as septicemia, toxic shock, pneumonia, and endocarditis with antibiotic resistance persistent nasal carriage normal individuals being key drivers of the medical impact this virulent pathogen. In both infection colonization, S. encounters host immune system produces a wide array factors that frustrate immunity. One particular, prototypical staphylococcal superantigen-like protein SSL7, potently binds IgA C5, thereby...
A considerable body of evidence suggests that Fc-dependent functions improve the capacity broadly neutralizing antibodies (BnAbs) to protect against and control HIV-1 infection. This phenomenon, however, has not been formally tested in robust cell-associated macaque simian-human immunodeficiency virus (SHIV) models with newer-generation BnAbs. We studied both WT BnAb PGT121 a LALA mutant (which impaired functions) for their ability pigtail macaques from an i.v. high-dose SHIVSF162P3...
Studying HIV-infected individuals who control HIV replication (elite controllers [ECs]) enables exploration of effective anti-HIV immunity. Env-specific and non-Env-specific antibody-dependent cellular cytotoxicity (ADCC) may contribute to protection from progressive infection, but the evidence is limited. We recruited 22 ECs matched them with 44 viremic subjects. Env- Vpu-specific ADCC responses in sera were studied using a novel enzyme-linked immunosorbent assay (ELISA)-based dimeric...
Abstract Background RTS,S is the leading malaria vaccine candidate but only confers partial efficacy against in children. based on major Plasmodium falciparum sporozoite surface antigen, circumsporozoite protein (CSP). The induction of anti-CSP antibodies important for protection; however, it unclear how these protective function. Methods We quantified functional antibody responses healthy malaria-naive adults (N = 45) vaccinated with RTS,S/AS01. This included ability to mediate effector...
Abstract Background Sublingual immunotherapy (SLIT) for grass pollen allergy can modify the natural history of allergic rhinitis and is associated with increased allergen‐specific IgG 4 . competitively inhibits functional IgE on surface effector cells, such as mast cells basophils, from binding to allergens. To further understand important role memory B‐cell (Bmem) responses play in mediating beneficial effects SLIT, we assessed changes Bmem subsets induced by SLIT allergy. Methods Blood...