A5082924650- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- Fungal and yeast genetics research
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Nuclear Structure and Function
- Microtubule and mitosis dynamics
- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- DNA and Nucleic Acid Chemistry
- Plant Molecular Biology Research
- Cancer therapeutics and mechanisms
- Telomeres, Telomerase, and Senescence
- Genetics, Aging, and Longevity in Model Organisms
- Mitochondrial Function and Pathology
- RNA modifications and cancer
- Carcinogens and Genotoxicity Assessment
- PARP inhibition in cancer therapy
- Bacterial Genetics and Biotechnology
- Ubiquitin and proteasome pathways
- Plant Disease Resistance and Genetics
- Plant Reproductive Biology
- Genetic Neurodegenerative Diseases
- Pluripotent Stem Cells Research
Friedrich Miescher Institute
2015-2024
University of Lausanne
2023-2024
Swiss Cancer Center Léman
2022-2023
University of Basel
2013-2022
Child Trends
2016-2022
Swiss Institute of Comparative Law
1998-2022
History of Science Society
2022
University of Nottingham
2020
Cold Spring Harbor Laboratory
2019
Cornell University
2018
Little is known about the dynamics of chromosomes in interphase nuclei. By tagging four chromosomal regions with a green fluorescent protein fusion to lac repressor, we monitored movement and subnuclear position specific sites yeast genome, sampling at short time intervals. We found that early late origins replication are highly mobile G 1 phase, frequently moving or faster than 0.5 micrometers/10 seconds, an energy-dependent fashion. The rapid diffusive chromatin detected becomes...
We present a new, robust, computational procedure for tracking fluorescent markers in time-lapse microscopy. The algorithm is optimized finding the time-trajectory of single particles very noisy dynamic (two- or three-dimensional) image sequences. It proceeds three steps. First, images are aligned to compensate movement biological structure under investigation. Second, particle's signature enhanced by applying Mexican hat filter, which we show be optimal detector Gaussian-like spot 1/omega2...
We have developed a novel technique for combined immunofluorescence/in situ hybridization on fixed budding yeast cells that maintains the three-dimensional structure of nucleus as monitored by focal sections labeled with fluorescent probes and staining nuclear pore antibody. Within resolution these immunodetection techniques, we show proteins encoded SIR3, SIR4, RAP1 genes colocalize in statistically significant manner Y' telomere-associated DNA sequences. In wild-type signals can be...
Recent findings suggest important roles for nuclear organization in gene expression. In contrast, little is known about how contributes to genome stability. Epistasis analysis (E-MAP) using DNA repair factors yeast indicated a functional relationship between pore subcomplex and Slx5/Slx8, small ubiquitin-like modifier (SUMO)-dependent ubiquitin ligase, which we show physically interact. Real-time imaging chromatin immunoprecipitation confirmed stable recruitment of damaged pores. Relocation...