A5035434845- DNA Repair Mechanisms
- Analytical Chemistry and Chromatography
- Genomics and Chromatin Dynamics
- Fungal and yeast genetics research
- Metabolism and Genetic Disorders
- Urological Disorders and Treatments
- Pharmacological Effects and Toxicity Studies
- Microtubule and mitosis dynamics
- Pediatric Urology and Nephrology Studies
- Analytical Methods in Pharmaceuticals
- Electrochemical sensors and biosensors
- Epilepsy research and treatment
- Amino Acid Enzymes and Metabolism
- Pharmacological Effects and Assays
- Circadian rhythm and melatonin
- Biotin and Related Studies
- Mass Spectrometry Techniques and Applications
- Receptor Mechanisms and Signaling
- Chemical Reactions and Isotopes
- Ureteral procedures and complications
- Carcinogens and Genotoxicity Assessment
- Sulfur Compounds in Biology
- Fatty Acid Research and Health
- RNA Research and Splicing
- Folate and B Vitamins Research
Friedrich Miescher Institute
2013-2024
Sumitomo Riko (Japan)
1995-2023
Hitachi (Japan)
1985-2019
Carnegie Mellon University
2017
Ryukoku University
2015
Hyogo Medical University
1983-2014
Niigata University of Pharmacy and Applied Life Sciences
1982-2006
Osaka Medical Center for Cancer and Cardiovascular Diseases
1992-2005
Niigata College of Technology
1989-2004
Nagano Red Cross Hospital
2004
Little is known about the dynamics of chromosomes in interphase nuclei. By tagging four chromosomal regions with a green fluorescent protein fusion to lac repressor, we monitored movement and subnuclear position specific sites yeast genome, sampling at short time intervals. We found that early late origins replication are highly mobile G 1 phase, frequently moving or faster than 0.5 micrometers/10 seconds, an energy-dependent fashion. The rapid diffusive chromatin detected becomes...
The intra-S-phase checkpoint in yeast responds to stalled replication forks by activating the ATM-like kinase Mec1 and CHK2-related Rad53, which turn inhibit spindle elongation late origin firing lead a stabilization of DNA polymerases at arrested forks. A mutation that destabilizes second subunit Origin Recognition Complex, orc2-1, reduces number functional 30% severely compromises activation Rad53 stress or damage S phase. We show restoration response correlates dose-dependent manner with...
Little is known about how cells ensure DNA replication in the face of RNA polymerase II (RNAPII)-mediated transcription, especially under conditions replicative stress. Here we present genetic and proteomic analyses from budding yeast that uncover links between checkpoint sensor Mec1–Ddc2 (ATR–ATRIP), chromatin remodeling complex INO80C (INO80 complex), transcription PAF1C (PAF1 complex). We found a subset chromatin-bound RNAPII degraded manner dependent on Mec1, INO80, PAF1 complexes...
The experiments were designed to study the well-known pigment-dependent mydriatic effect of atropine in eye. In vitro, relative accumulation 3H-atropine by nonpigmented rabbit iris, pigmented iris accumulated high amounts drug. A tissue, stomch fundus strip, obtained from either albino or nonalbino animals, relatively low 3H-atropine. On repeated washings, drug tissues was rapidly lost, T 1/2 14 minutes, while that retained much longer. Although vitro aqueous humor serum-atropinesterase...
The Dbf4/Cdc7 kinase acts at the level of individual origins to promote initiation DNA replication. We demonstrate through both immunoprecipitation and two-hybrid assays that a domain comprising first 296 aa Dbf4p interacts with Orc2p Orc3p subunits origin recognition complex (ORC). Given activation Rad53 in response replication checkpoint leads release from an ORC-containing chromatin fraction, we also examined interaction between Rad53p. This same binds specifically forkhead...
Checkpoints monitor critical cell cycle events such as chromosome duplication and segregation. They are highly conserved mechanisms that prevent progression into the next phase of when cells unable to accomplish previous event properly. During S phase, also provide a surveillance mechanism called DNA replication checkpoint, which consists kinase cascade is provoked by insults block or slow down forks. The checkpoint crucial for maintaining genome stability, because forks become vulnerable...
Bacteria are able to repair ultraviolet light (UV)-induced damage their DNA in the absence of visible light. This process is called dark repair, and it has been established that some these processes an enzymatic nature. Boyce Howard-Flanders (1964), Setlow Carrier Pettijohn Hanawalt (1964) postulated may proceed as illustrated Fig. 1: (1) a single-strand fragment containing photoproducts such thymine dimers released from UV-irradiated DNA; (2) new nucleotides complementary those intact...
The encoding and evolution of specificity affinity in protein-protein interactions is poorly understood. Here, we address this question by quantifying how all mutations one protein, JUN, alter binding to other members a protein family, the 54 human basic leucine zipper transcription factors. We fit global thermodynamic model data reveal that most changing equally affect JUN's its interaction partners. Mutations are relatively rare but distributed throughout interface. Specificity determined...
Actin-related proteins are ubiquitous components of chromatin remodelers and conserved from yeast to man. We have examined the role budding actin-related protein Arp6 in gene expression, both as a component SWR1 complex (SWR-C) its absence. mapped binding sites along four chromosomes using immunoprecipitation wild-type swr1 deleted (swr1Delta) cells. find that majority coincide with Swr1, catalytic subunit SWR-C, histone H2A variant Htz1 (H2A.Z) deposited by SWR-C. However, detected at...