A5031848923- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Histone Deacetylase Inhibitors Research
- Galectins and Cancer Biology
- Chemical Synthesis and Analysis
- RNA Research and Splicing
- interferon and immune responses
- Carbohydrate Chemistry and Synthesis
- Toxin Mechanisms and Immunotoxins
- Peptidase Inhibition and Analysis
- RNA regulation and disease
- Influenza Virus Research Studies
- Cancer, Hypoxia, and Metabolism
- Plant Reproductive Biology
- Signaling Pathways in Disease
- ATP Synthase and ATPases Research
- Fungal and yeast genetics research
- S100 Proteins and Annexins
- Escherichia coli research studies
- Cytokine Signaling Pathways and Interactions
- Pancreatic function and diabetes
- Drug Transport and Resistance Mechanisms
- T-cell and B-cell Immunology
- Fungal Biology and Applications
- bioluminescence and chemiluminescence research
Friedrich Miescher Institute
2006-2019
Frequency Management International (United States)
2015
Ames National Laboratory
2006
Washington University in St. Louis
2000
University College London
1995-1999
Copper chelators could help to reduce metastasis from breast tumors.
The mechanisms controlling cell fate determination and reprogramming are fundamental for development. A profound reprogramming, allowing the production of pluripotent cells in early embryos, takes place during oocyte-to-embryo transition. To understand how oocyte potential is controlled, we sought Caenorhabditis elegans mutants which embryonic transcription initiated precociously germ cells. This screen identified LIN-41, a TRIM-NHL protein component somatic heterochronic pathway, as...
We have cloned Gb<sub>3</sub>synthase, the key α1,4-galactosyltransferase in globo-series glycosphingolipid (GSL) synthesis, via a phenotypic screen, which previously yielded iGb<sub>3</sub> synthase, α1,3-galactosyltransferase required isoglobo-series GSL (Keusch, J. J., Manzella, S. M., Nyame, K. A., Cummings, R. D., and Baenziger, U. (2000) <i>J. Biol. Chem.</i>33). Both transferases act on lactosylceramide, Galβ1,4Glcβ1Cer (LacCer), to produce Gb<sub>3</sub>(Galα1,4LacCer) or...
Thrombospondin type 1 repeats (TSRs) are biologically important domains of extracellular proteins. They modified with a unique Glcβ1,3Fucα1-O-linked disaccharide on either serine or threonine residues. Here we identify the putative glycosyltransferase, B3GTL, as β1,3-glucosyltransferase involved in biosynthesis this disaccharide. This enzyme is conserved from Caenorhabditis elegans to man and shares 28% sequence identity Fringe, β1,3-N-acetylglucosaminyltransferase that modifies O-linked...
Peters Plus syndrome is an autosomal recessive disorder characterized by anterior eye chamber defects, disproportionate short stature, developmental delay, and cleft lip and/or palate. It caused splice site mutations in what was thought to be a beta1,3-galactosyltransferase-like gene (B3GALTL). Recently, we others found this encode beta1,3-glucosyltransferase involved the synthesis of disaccharide Glc-beta1,3-Fuc-Omicron-that occurs on thrombospondin type 1 repeats many biologically...
Acyl coenzyme A (acyl-CoA) thioesterases hydrolyze thioester bonds in acyl-CoA metabolites. The majority of mammalian are α/β-hydrolases and have been studied extensively. second class Hotdog-fold enzymes has less well described. Here, we present a structural functional analysis new mitochondrial thioesterase, Them5. Them5 its paralog, Them4, adopt the classical structure form homodimers crystals. In vitro, shows strong thioesterase activity with long-chain acyl-CoAs. Loss specifically...
RNA-binding proteins regulate all aspects of RNA metabolism. Their association with is mediated by domains, which many remain uncharacterized. A recently reported example the NHL domain, found in prominent regulators cellular plasticity like C. elegans LIN-41. Here we employ an integrative approach to dissect specificity Using computational analysis, structural biology, and vivo studies worms human cells, find that a positively charged pocket, specific domain LIN-41 its homologs...
An absence of galactose on the N-linked oligosaccharides immunoglobulin G (IgG) has been shown to affect functional activity antibody molecule. In patients with rheumatoid arthritis there is an increased proportion IgG which lacks and correspondingly lower levels β1,4-galactosyltransferase (β4Gal-T) activity. The recent demonstration several expressed β4Gal-T genes in man raises possibility that enzyme responsible for decreased not "classical" (β4Gal-T1). To directly address question whether...
Lymphocytic β1,4-galactosyltransferase (β1,4-GalTase, EC 2.4.1.38) activity was measured in B cells using a neoglycoprotein, N-acetylglucosamine-phenylisothlocyanate-bovine serum albumin (GlcNAc-pITC-BSA), as an acceptor substrate novel enzyme-linked immunosorbent assay (ELISA)-based method. This proved to be much simpler use than the lengthy and expensive radiochemical assays commonly used, has additional advantage that it specifically detects enzyme mediating transfer via Galβ1,4GlcNAc...
The effect on murine immunoglobulin G (IgG) glycosylation of altering IgG production in vivo was assessed interleukin (IL)-6 transgenic and CD4 knockout mice. C57BL/6 mice carrying the IL-6 transgene showed increased levels circulating IgG. This associated with decreased galactose oligosaccharides. No decrease beta4-galactosyltransferase mRNA or enzyme activity seen MRL-lpr/lpr normally have elevated IgG, again accompanied by galactose. Disruption gene led to a substantial concentration but...