A5069195667- DNA Repair Mechanisms
- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Fungal and yeast genetics research
- Nuclear Structure and Function
- Cancer-related molecular mechanisms research
- Plant Reproductive Biology
- Carcinogens and Genotoxicity Assessment
- Ovarian cancer diagnosis and treatment
- Endometriosis Research and Treatment
- Advanced Electron Microscopy Techniques and Applications
- RNA Research and Splicing
- Chromosomal and Genetic Variations
- DNA and Nucleic Acid Chemistry
- Photosynthetic Processes and Mechanisms
- CRISPR and Genetic Engineering
- Cellular transport and secretion
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Plant Virus Research Studies
- Technology Use by Older Adults
- Ubiquitin and proteasome pathways
- Reproductive System and Pregnancy
- Metalloenzymes and iron-sulfur proteins
- Epigenetics and DNA Methylation
Friedrich Miescher Institute
2019-2024
Paul Scherrer Institute
2021-2024
Protein Research Foundation
2018-2023
Osaka University
2014-2023
Osmania University
2014
Chromosome 18 Registry & Research Society
2014
University of Hyderabad
2013
Centre for Cellular and Molecular Biology
2012
Sister chromatid cohesion on chromosome arms is essential for the segregation of homologous chromosomes during meiosis I while it dispensable sister separation mitosis. It was assumed that, unlike situation in mitosis, retain prior to onset anaphase-I. Paradoxically, reduced immunostaining signals meiosis-specific cohesin, including kleisin Rec8, were observed late prophase-I budding yeast. This decrease seen absence Rec8 cleavage and depends condensin-mediated recruitment Polo-like kinase...
Meiosis-specific cohesin, required for the linking of sister chromatids, plays a critical role in various chromosomal events during meiotic prophase I, such as chromosome morphogenesis and dynamics, well recombination. Rad61/Wpl1 (Wapl other organisms) negatively regulates cohesin functions. In this study, we show that axes are shortened budding yeast rad61/wpl1 mutant, suggesting axis compaction. is efficient resolution telomere clustering meiosis indicating positive effect on function...
Upon replication stress, budding yeast checkpoint kinase Mec1 ATR triggers the downregulation of transcription, thereby reducing level RNA polymerase (RNAP) on chromatin to facilitate fork progression.Here, we identify a hydroxyurea-induced phosphorylation site Mec1, Mec1-S1991, that contributes eviction RNAPII and RNAPIII during stress.The expression non-phosphorylatable mec1-S1991A mutant reduces progression genome-wide compromises survival hydroxyurea.This defect can be suppressed by...
DNA2 is an essential nuclease-helicase implicated in DNA repair, lagging-strand synthesis, and the recovery of stalled replication forks (RFs). In Saccharomyces cerevisiae, dna2Δ inviability reversed by deletion conserved helicase PIF1 and/or damage checkpoint-mediator RAD9. It has been suggested that Pif1 drives formation long 5'-flaps during Okazaki fragment maturation, function Dna2 to remove these intermediates. absence Dna2, are thought accumulate on lagging strand, resulting...
On induction of DNA damage with 405-nm laser light, proteins involved in base excision repair (BER) are recruited to lesions. We find that the dynamics factors typical either short-patch (XRCC1) or long-patch (PCNA) BER altered by chemicals perturb actin tubulin polymerization human cells. Whereas destabilization filaments latrunculin B, cytochalasin Jasplakinolide decreases factor accumulation at laser-induced damage, inhibition nocodazole increases it. detect no recruitment sites yet...
Abstract Loss of cytosolic actin filaments upon TORC2 inhibition triggers chromosome fragmentation in yeast, which results from altered base excision repair Zeocin-induced lesions. To find the link between kinase and this yeast shattering (YCS) we performed phosphoproteomics. YCS-relevant phospho-targets included plasma membrane-associated regulators polymerization, such as Las17, Wiscott-Aldrich Syndrome protein. Induced degradation Las17 was sufficient to trigger YCS presence Zeocin,...
During meiosis, protein ensembles in the nuclear envelope (NE) containing SUN- and KASH-domain proteins, called linker nucleocytoskeleton cytoskeleton (LINC) complex, promote chromosome motion. Yeast SUN-domain protein, Mps3, forms multiple meiosis-specific on NE, which show dynamic localisation for motion; however, mechanism by these Mps3 are formed during meiosis remains largely unknown. Here, we showed that cyclin-dependent kinase (CDK) Dbf4-dependent Cdc7 (DDK) regulate dynamics of...
Abstract Multiple genomic and proteomic studies have suggested that peripheral blood mononuclear cells (PBMCs) respond to tumor secretomes thus could provide possible avenues for prognosis treatment evaluation. We hypothesized the chromatin organization of PBMCs obtained from liquid biopsies, which integrates secretome signals with gene expression programs, provides efficient biomarkers characterize efficacy proton therapy in patients. Here, we show imaging combined machine learning methods...
Proteins in the nuclear envelope (NE) play a role dynamics and functions of nucleus chromosomes during mitosis meiosis. Mps3, yeast NE protein with conserved SUN domain, predominantly localizes on centrosome equivalent, spindle pole body (SPB), mitotic cells. During meiosis, together SPB, forms distinct multiple ensemble NE. How meiosis-specific localization Mps3 is regulated remains largely unknown. In this study, we found that component complex essential for sister chromatid cohesion,...
The mitotic cohesin complex necessary for sister chromatid cohesion and chromatin loop formation shows local global association to chromosomes in response DNA double-strand breaks (DSBs). Here, by genome-wide binding analysis of the meiotic with Rec8, we found that Rec8-localization profile along is altered from middle late prophase I cleavage-independent dissociation. Each Rec8-binding site on chromosome axis follows a unique alternation pattern dissociation probably association....
Abstract Sister chromatid cohesion on chromosome arms is essential for the segregation of homologous chromosomes during meiosis I while it dispensable sister separation mitosis. It was assumed that, unlike situation in mitosis, retain prior to onset anaphase-I. Paradoxically, reduced immunostaining signals meiosis-specific cohesin, including kleisin Rec8, from were observed late prophase-I budding yeast. This decrease seen absence Rec8 cleavage and depends condensin-mediated recruitment...
Abstract Proper repair of double-strand breaks (DSBs) is key to ensure proper chromosome segregation. In this study, we found that the deletion SRS2 gene, which encodes a DNA helicase necessary for control homologous recombination, induces aberrant segregation during budding yeast meiosis. This abnormal in srs2 cells accompanies formation novel damage induced late meiotic prophase-I. The may contain long stretches single-stranded DNAs (ssDNAs), lead aggregate ssDNA binding protein, RPA, and...
Abstract During meiosis, protein ensembles in the nuclear envelope (NE) containing SUN- and KASH-domain proteins, called linker nucleocytoskeleton cytoskeleton (LINC) complex, promote chromosome motion. Yeast SUN-domain protein, Mps3, forms multiple meiosis-specific on NE, which show dynamic localisation for motion; however, mechanism by these Mps3 are formed during meiosis remains largely unknown. Here, we showed that cyclin-dependent kinase (CDK) Dbf4-dependent Cdc7 (DDK) regulate dynamics...