A5043592767- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Chronic Lymphocytic Leukemia Research
- Glycosylation and Glycoproteins Research
- Immunodeficiency and Autoimmune Disorders
- Lymphoma Diagnosis and Treatment
- Systemic Lupus Erythematosus Research
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Galectins and Cancer Biology
- Mycobacterium research and diagnosis
- Bacterial Infections and Vaccines
- Streptococcal Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Heat shock proteins research
- Viral-associated cancers and disorders
- Vector-borne infectious diseases
- Rheumatoid Arthritis Research and Therapies
- Microbial infections and disease research
- Fungal Infections and Studies
- Clostridium difficile and Clostridium perfringens research
- Leptospirosis research and findings
- Zoonotic diseases and public health
University of Westminster
2008-2024
University of Georgia
2024
University of Georgia
2021-2024
University College London
1996-2010
The Royal Free Hospital
1999-2010
MRC Human Immunology Unit
2001
Kingston University
1999
Centre Hospitalier Régional Universitaire de Brest
1987-1999
University College Hospital
1993-1996
Middlesex University
1988-1994
Subpopulations of thymus-derived T lymphocytes bearing receptors for either IgM or IgG molecules were isolated from human peripheral blood. Those with (T.M) provided help in a cell dose-dependent fashion the pokeweed mitogen-induced differentiation B vitro, whereas cells (T.G) did not. T.G cells, on hand, efficiently suppressed and proliferation system presence helper T.M cells. This suppressive activity required prior interaction immune complexes. The was relatively radioresistant while...
The initial development and distribution of lymphocytes expressing surface IgM (sIgM) specific antigen-binding cells (ABC) were studied in the chicken an attempt to gain information on process by which B-cell diversity is generated. antigens used sheep erythrocytes (SE), keyhole limpet hemocyanin (KLH), poly-L(Tyr, Glu)poly-D,L-Ala-poly-L-Lys (TGAL). results indicate that generation total sIgM-positive population begins bursa clones ABC develop a fixed sequential pattern not influenced...
Concanavalin A (Con A) stimulated the IgM-binding subpopulation of human T cells (TM) to suppress pokeweed mitogen-induced differentiation B lymphocytes plasma cells. Control TM that had not been Con activated did suppress. The degree suppression was related number A-TM added cultures and it abolished by irradiation either before or after 24-hr culture period with A. Suppression require presence TG cells, whose suppressor potential has previously established. These findings indicate activity...
Autoantigens cross reactive with mycobacteria are implicated in the pathogenesis of adjuvant arthritis rat, and there reports changes immune response to human rheumatoid (RA). We have therefore examined IgM, IgG, IgA antibody levels crude mycobacterial antigens two recombinant heat shock/stress proteins (65 kD 71 kD) sera from patients RA, systemic lupus erythematosus (SLE), Crohn9s disease, healthy controls. binding was significantly raised RA sera, though IgG IgM tended be lower than Both...
Abstract Abnormal proportions of the distinct T cell subpopulations binding Fc portion IgM (T ·M ) cells and those bearing receptors for IgG cells, were observed in blood samples from patients who had congenital or acquired abnormalities thymus, severe combined immunodeficiency, an unexplained primary deficiency cell‐mediated immunity; most too few circulating often overabundance ·G cells. In vitro evaluation lymphocytes one three thymoma with elevated subpopulation, removal abrogated...
Objective Small IgG rheumatoid factor immune complexes may provide the trigger for macrophage-derived tumor necrosis α (TNFα) production in arthritis. Immune bind to any of 3 Fc receptors (FcγR). Therefore, ability monocyte-derived macrophages produce TNFα was examined following ligation each human FcγR, using murine monoclonal antibodies (mAb) receptor as a model small complexes. Methods Adhered monocytes expressing all FcγR were incubated with anti-FcγR mAb directed against FcγRI, FcγRII,...
We have previously demonstrated raised levels of IgG and IgA antibody to the mycobacterial 65‐kDa heat shock protein (hsp) in sera patients with rheumatoid arthritis (RA). now attempted determine whether this phenomenon is specific for RA, it seen only homologue particular hsp gene family. therefore screened Escherichia coli 65, mycobacterial, E. , human 70, from systemic lupus erythematosus (SLE), tuberculosis (TB), ankylosing spondylitis (AS), Crohn's disease, control donors. RA show...
Ideas about possible mechanisms for the generation of B-cell diversity have been deduced almost exclusively from analysis immunoglobulin products mature B cells. A developmental approach to this problem has recently become through discovery unique role bursa Fabricius as a source cells in birds and by development methods model systems which very young mammals can be examined. Sufficient results different approaches are now available begin an integration two basic types information show their...
In search of markers disease activity in patients with SLE we have investigated blood lymphocyte subsets from a large cohort patient. Seventy-one were studied using well-defined panel fluorescent monoclonal antibodies which recognize the major T, B and NK activated cells. Flow cytometry was used standard automated software. Overall, lymphopenic. The proportion T cells increased decreased both absolute numbers ( P < 0.001). This decrease more pronounced active patients. None cell...
antibodies from theresulting lines and clones were examined for their binding to a variety of auto‐antigens micro‐organisms by ELISA fluorescence assays. Auto‐antigens tested included Fc IgG, ssDNA dsDNA, cardiolipin, histones 1‐4, collagens type I II. thyroglobulin, cytoskeletal components, tissue section screen. Of 71 cell tested, all but 19 showed some autoreactivity. All 32 fetal liver reacted seif‐antigens. In cord blood clones, 16 out 26 bound auto‐antigens. Many the with more than one...
The clonality of T lymphocytes isolated from the synovial fluid and peripheral blood patients with rheumatoid arthritis was investigatcd by restriction enzyme fragment mapping rearrangement is β chain gene T‐cell antigen receptor. Three showed a dominant amongst their cells which not seen in population, suggesting prescnce predominating clone. However, most examined (8 out 11) demonstrated polyclonal populations both blood. Of four lines investigated, one evidence
We have examined the frequencies of Tγδ cells in blood, synovial fluids, and membranes patients with rheumatoid arthritis (RA) blood from age‐matched controls Immunocyto‐chemical immunohistochemical techniques were used monoclonal antibodies BB3 A13 to define a major minor subset respectively. Together, these identify majority (if not all) peripheral cells. Significantly lower levels found RA compared controls, whilst higher but significant numbers fluids or paired samples Scattered only...
OBJECTIVES--To determine which heat shock proteins (hsps) are overexpressed in systemic lupus erythematosus (SLE), and to examine the relevance of these findings clinical disease activity. METHODS--Hsp levels peripheral blood mononuclear cells (PBMC) patients with SLE normal controls were measured. Levels analysed respect detailed activity scores. Other hsps also quantified 30-50% samples. RESULTS--There was significant increase 90 kilodalton protein (hsp90) active neuropsychiatric (p <...