A5047782310- Herpesvirus Infections and Treatments
- Immune Cell Function and Interaction
- Immunodeficiency and Autoimmune Disorders
- T-cell and B-cell Immunology
- Cytomegalovirus and herpesvirus research
- Diabetes and associated disorders
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Animal Virus Infections Studies
- Immune Response and Inflammation
- Health and Medical Research Impacts
- Poxvirus research and outbreaks
- Parasitic Infections and Diagnostics
- Pancreatic function and diabetes
- Blood disorders and treatments
- Monoclonal and Polyclonal Antibodies Research
- Diabetes Management and Research
- Infant Nutrition and Health
- Dermatology and Skin Diseases
- Health Sciences Research and Education
- Theater, Performance, and Music History
- CAR-T cell therapy research
- Neonatal Respiratory Health Research
- Bartonella species infections research
- Toxoplasma gondii Research Studies
Rhode Island Hospital
2016-2025
Brown University
2016-2025
Providence College
2016-2021
John Brown University
2019-2021
Hasbro Children's Hospital
2016-2021
University of Colorado Denver
1994-2010
National Institutes of Health
2002-2009
Weatherford College
2009
Singer (United States)
2009
Child Trends
2009
The incidence and severity of herpes zoster postherpetic neuralgia increase with age in association a progressive decline cell-mediated immunity to varicella-zoster virus (VZV). We tested the hypothesis that vaccination against VZV would decrease incidence, severity, or both among older adults.We enrolled 38,546 adults 60 years randomized, double-blind, placebo-controlled trial an investigational live attenuated Oka/Merck vaccine ("zoster vaccine"). Herpes was diagnosed according clinical...
BackgroundA double-blind, placebo-controlled trial that involved 38,546 subjects ⩾60 years old demonstrated efficacy of a high-potency live-attenuated Oka/Merck varicella-zoster virus (VZV) vaccine. The included an immunology substudy to determine the relationship VZV-specific immune responses vaccination and clinical outcome MethodsThe enrolled 1395 at 2 sites where blood samples obtained prior vaccination, 6 weeks after 1, 2, 3 thereafter were tested for cell-mediated immunity (VZV-CMI) by...
Human recombination-activating gene (RAG) deficiency can manifest with distinct clinical and immunological phenotypes. By applying a multiomics approach to large group of RAG-mutated patients, we aimed at characterizing the immunopathology associated each phenotype. Although defective T B cell development is common all phenotypes, patients hypomorphic RAG variants generate cells signatures immune dysregulation produce autoantibodies broad range self-antigens, including type I interferons....
We report an association between X-linked immunodeficiency with hyper-IgM (XHIM) and carcinomas affecting the liver, pancreas, biliary tree, associated neuroectodermal endocrine cells. The tumors were fatal in eight of nine cases most instances preceded by chronic cholangiopathy and/or cirrhosis. An additional group subjects XHIM had inflammation liver or bile ducts but no malignancy. Many patients infected cryptosporidia. CD40 is normally expressed on regenerating inflammed duct epithelium....
The safety and immunogenecity of a booster dose live attenuated varicella-zoster virus (VZV) vaccine was evaluated in 196 healthy subjects, >or=60 years old, who had already received VZV >5 before. This repeat well tolerated. Cell-mediated immunity (CMI) to measured by an interferon-gamma (IFN-gamma) enzyme-linked immunosorbent spot-forming cell (ELISPOT) assay limiting dilution responder frequency (RCF) assay. Prevaccination responses decreased as function increasing age but were detectable...
BackgroundThe objectives of this study were to evaluate the association between varicella-zoster virus (VZV)–specific humoral and cell-mediated immunity (CMI) herpes zoster (HZ) protection against HZ morbidity compare immune responses vaccine MethodsIn 981 elderly persons who developed during a efficacy trial (321 vaccinees 660 placebo recipients) 1362 without (682 680 recipients), CMI was measured by VZV responder cell frequency interferon-γ enzyme-linked immunospot, antibodies...
Abstract Resembling the in vitro antibody response of newborn, cultures cord blood lymphocytes stimulated with pokeweed mitogen (PWM) generated fewer plasma cells (PC) than comparable adult lymphocyte and was almost exclusively IgM class. We investigated cellular basis this difference by preparing mixed newborn T or B cells. Substitution for enhanced cells, particularly IgG IgA classes. The second trimester fetal spleen also increased although no PC appeared. Conversely, classes when...
Concanavalin A (Con A) stimulated the IgM-binding subpopulation of human T cells (TM) to suppress pokeweed mitogen-induced differentiation B lymphocytes plasma cells. Control TM that had not been Con activated did suppress. The degree suppression was related number A-TM added cultures and it abolished by irradiation either before or after 24-hr culture period with A. Suppression require presence TG cells, whose suppressor potential has previously established. These findings indicate activity...
Journal Article Immune Response of Elderly Individuals to a Live Attenuated Varicella Vaccine Get access Myron J. Levin, Levin Reprints or correspondence: Dr. University Colorado School Medicine, 4200 E. 9th Ave.-Campus Box C-227, Denver, CO, 80262 Search for other works by this author on: Oxford Academic PubMed Google Scholar Martha Murray, Murray Harley A. Rotbart, Rotbart Gary O. Zerbe, Zerbe C. Jo White, White Anthony R. Hayward The Infectious Diseases, Volume 166, Issue 2, August 1992,...
Varicella-zoster virus (VZV)-specific T cell immunity was measured in 130 persons > or = 55 years of age 6 after they received a live attenuated VZV vaccine. Circulating cells, which proliferated vitro response to antigen, were enumerated (VZV responder frequency assay). Six the booster vaccination, VZV-responding (1/61,000 circulating cells) still significantly (P < .05) improved over baseline measurements (1/70,000) and appears have diminished expected decline as these vaccinees aged (to...
Abstract Human T lymphocytes have been tested for cell surface p 28,33 “Ia‐like” heteroantigen and DRw alloantigens. Small numbers (1–5%) of sheep (E) rosette or antigen‐positive, immunoglobulin‐negative (E + , smIg − ) cells were Ia ; these appeared to be restricted the G subset. Following activation by allogeneic sperm, purified protein derivative tuberculin (PPD), proportion positive increased substantially. typing indicated that specificities on activated not acquired passively from...
Background. Varicella-zoster virus (VZV)-specific cell-mediated immunity is important for protection against VZV disease. We studied the relationship between and age after varicella or vaccination in healthy human immunodeficiency (HIV)-infected individuals. Methods. responder cell frequency (RCF) determinations from 752 200 HIV-infected subjects were used to identify group-specific regression curves on age. Results. In individuals with past varicella, RCF peaked at 34 years of Similarly,...
Abstract The frequency of blood mononuclear cells expressing the 180‐kDa splice variant common leukocyte antigen identified by UCHL1 monoclonal antibody (a characteristic post‐thymic which have been through a cycle stimulation) rose in logarithmic relationship to age between birth and 16 years. At only low CD4 + (negative for CD 25) no CD8 T expressed UCHL1. subsequent increase expression occurred parallel on α/β receptors γ/δ receptors, suggesting that these subsets participated postthymic...
The incidence of herpes zoster increases markedly with advancing age, and this appears to be causally related an age-dependent decline in varicella-zoster virus (VZV)—specific cellular immunity. Psychologic stress has also been linked the occurrence zoster, but mechanism involved not investigated. This study examined relationship between major depression VZV-specific immunity by comparing responder cell frequency (RCF) adults (n = 11) that age- sex-matched nondepressed controls a larger...
Over 80% of nonobese diabetic (NOD) mice develop lymphocytic infiltrates their pancreatic islets (insulitis) by 6 wk age and 50% the females are mo age. The incidence insulitis in NOD injected once as neonates with 250 micrograms CD3 antibody, 145.2C11, was 8% at 10 age, increasing to 25% 32 Fewer than 10% these animals developed diabetes 8 Neonatal administration 145.2C11 reduced proliferative responses spleen cells mitogen stimulation 2 4 postinjection expression TCR 1 5 postinjection....
ABSTRACT Mice with disrupted genes for CD40 and ligand (CD40L) are unable to clear infection Cryptosporidium parvum develop cholangitis. Parasites present in the gut, gall bladder, biliary tree, epithelial cells express on cell surface. SCID mice infected C. >1 month can after reconstitution spleen from CD40, but not CD40L, knockout mice. In an vitro model, -infected HepG2 were triggered apoptosis when incubated a CD40L-CD8 fusion protein. The requirement CD40-CD40L interactions immunity...