A5027155102- Genomics and Chromatin Dynamics
- Genetics, Aging, and Longevity in Model Organisms
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Nuclear Structure and Function
- DNA Repair Mechanisms
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Microtubule and mitosis dynamics
- Fungal and yeast genetics research
- Medieval European History and Architecture
- Historical Influence and Diplomacy
- Historical, Literary, and Cultural Studies
- Single-cell and spatial transcriptomics
- RNA and protein synthesis mechanisms
- Protist diversity and phylogeny
- Pluripotent Stem Cells Research
- Medieval Literature and History
- Heat shock proteins research
- Evolution and Genetic Dynamics
- German Colonialism and Identity Studies
- Carcinogens and Genotoxicity Assessment
- Gene Regulatory Network Analysis
- Health, psychology, and well-being
University of Bern
2015-2024
Institute of Cell Biology
2015-2021
Universidad Pablo de Olavide
2014
Friedrich Miescher Institute
2006-2013
Institut Curie
2003-2007
Centre National de la Recherche Scientifique
2003-2007
Gemeinschaftskrankenhaus Herdecke
2007
Abstract Chromosome structure in mammals is thought to regulate transcription by modulating three-dimensional interactions between enhancers and promoters, notably through CTCF-mediated loops topologically associating domains (TADs) 1–4 . However, how chromosome are actually translated into transcriptional outputs remains unclear. Here, address this question, we use an assay position enhancer at large numbers of densely spaced chromosomal locations relative a fixed promoter, measure promoter...
To understand whether the spatial organization of genome reflects cell's differentiated state, we examined genes assume specific subnuclear positions during Caenorhabditis elegans development. Monitoring radial position developmentally controlled promoters in embryos and larval tissues, found that small integrated arrays bearing three different tissue-specific have no preferential nuclei undifferentiated embryos. However, cells, they shifted stably toward nuclear lumen when activated, or to...
Some inducible yeast genes relocate to nuclear pores upon activation, but the general relevance of this phenomenon has remained largely unexplored. Here we show that bidirectional hsp-16.2/41 promoter interacts with pore complex activation by heat shock in nematode Caenorhabditis elegans. Direct association was confirmed both super-resolution microscopy and chromatin immunoprecipitation. The hsp-16.2 sufficient mediate perinuclear positioning under basal level conditions expression,...
Abstract Chromosome structure in mammals is thought to regulate transcription by modulating the three-dimensional interactions between enhancers and promoters, notably through CTCF-mediated topologically associating domains (TADs) 1–4 . However, how chromosome are actually translated into transcriptional outputs remains unclear. To address this question we use a novel assay position an enhancer at large number of densely spaced chromosomal locations relative fixed promoter, measure promoter...
In response to DNA damage and replication pausing, eukaryotes activate checkpoint pathways that prevent genomic instability by coordinating cell cycle progression with repair. The intra-S-phase has been proposed protect stalled forks from pathological rearrangements could result unscheduled recombination. On the other hand, recombination may be needed cope either or double-strand breaks resulting hydroxyurea treatment. We have exploited fission yeast elucidate relationship between fork...
We describe a straightforward two-step PCR-based method to insert arrays of lac or tet operators (lacO tetO) at specific loci in the budding yeast genome. The entails insertion marker generated by PCR with classical long primers recognizing locus interest, followed replacement this linearized plasmid bearing an array lacI- tetR-binding motifs. Using technique, located either genome on artificial chromosomes can be efficiently tagged. provide set plasmids different markers for cloning-free...
B.D. Towbin, P. Meister, B.L. Pike and S.M. Gasser⇓ Friedrich Miescher Institute for Biomedical Research, CH-4058 Basel, Switzerland Correspondence: susan.gasser{at}fmi.ch
Heterochromatin protein 1 (HP1) family proteins have a well-characterized role in heterochromatin packaging and gene regulation. Their function organismal development, however, is less well understood. Here we used genome-wide expression profiling to assess novel functions of the Caenorhabditis elegans HP1 homolog HPL-2 at specific developmental stages.We show that regulates germline genes, extracellular matrix components genes involved lipid metabolism. Comparison our data with ChIP-on-chip...
The adjustment of X-linked gene expression to the X chromosome copy number (dosage compensation [DC]) has been widely studied as a model chromosome-wide regulation. In Caenorhabditis elegans , DC is achieved by twofold down-regulation from both Xs in hermaphrodites. We show that males, single interacts with nuclear pore proteins, while hermaphrodites, complex (DCC) impairs this interaction and alters localization. Our results put forward structural which X-specific sequences locate...
Abstract Transcriptional enhancers must find their target genes both efficiently and specifically. Chromatin conformation capture revealed the critical function of three-dimensional chromosome segmentation by topologically associated domains (TADs) to limit search space for promoters in mammals. In nematodes, although more than 30’000 sequences with characteristic enhancer chromatin features have been identified, autosomal genome is not segmented TADs, raising question mechanism directing...
In mammalian and budding yeast cells treated with genotoxic agents, different proteins implicated in detecting, signalling or repairing DNA lesions form nuclear foci. We studied foci formed by involved these processes living fission cells, which is amenable to genetic molecular analysis. Using fluorescent tags, we analysed subnuclear localisations of the damage checkpoint protein Rad9, homologous recombination Rad22 PCNA, are many aspects metabolism. After inducing double strand breaks...
Parkinson's disease (PD) is the most common neurodegenerative movement disorder characterized by progressive loss of dopaminergic (DA) neurons. Both environmental and genetic factors are thought to contribute pathogenesis PD. Although several genes linked rare familial PD have been identified, endogenous risk for sporadic PD, which account majority cases, remain largely unknown. Genome-wide association studies identified many single nucleotide polymorphisms associated with in...
We report the reproductive strategy of nematode Mesorhabditis belari This species produces only 9% males, whose sperm is necessary to fertilize and activate eggs. However, most fertilized eggs develop without using DNA produce female individuals. Only in male utilized, producing sons. found that mixing parental genomes gives rise males because Y-bearing are much more competent than X-bearing for penetrating In this previously unrecognized strategy, asexual females few sexual genes never...
Differential gene expression across cell types underlies development and physiology in multicellular organisms. Caenorhabditis elegans is a powerful, extensively used model to address these biological questions. A remaining bottleneck relates the difficulty obtain comprehensive tissue-specific transcription data, since available methods are still challenging execute and/or require large worm populations. Here, we introduce RNA Polymerase DamID (RAPID) approach, which Dam methyltransferase...