A5069444674- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Nuclear Structure and Function
- DNA Repair Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Sarcoma Diagnosis and Treatment
- Soft tissue tumor case studies
- Congenital heart defects research
- Plant Reproductive Biology
- CRISPR and Genetic Engineering
- Cellular Mechanics and Interactions
- Chromatin Remodeling and Cancer
- Developmental Biology and Gene Regulation
- Protein Degradation and Inhibitors
- Oral and Maxillofacial Pathology
- Cancer Genomics and Diagnostics
- RNA regulation and disease
- Vascular Tumors and Angiosarcomas
- Ubiquitin and proteasome pathways
- Telomeres, Telomerase, and Senescence
Centro Andaluz de Biología Molecular y Medicina Regenerativa
2008-2023
Universidad Pablo de Olavide
2012-2023
Universidad de Sevilla
2008-2023
University of Copenhagen
2014-2020
National Research Council
2020
Instituto de Biomedicina de Sevilla
2020
Consejo Superior de Investigaciones Científicas
2011-2014
Centro Andaluz de Biología del Desarrollo
2012
University of Massachusetts Chan Medical School
2012
Salk Institute for Biological Studies
2012
The eukaryotic THO/TREX complex, involved in mRNP biogenesis, plays a key role the maintenance of genome integrity yeast. mRNA export factors such as Thp1-Sac3 also affect integrity, but their mutations have other phenotypes different from those THO/TREX. Sus1 is novel component SAGA transcription factor that associates with Thp1-Sac3, little known about its effect on instability and transcription. Here we show Thp1, Sac3, form functional unit biogenesis independent SAGA. Importantly,...
The evolutionarily conserved protein Sem1/Dss1 is a subunit of the regulatory particle (RP) proteasome, and, in mammalian cells, binds tumor suppressor BRCA2. Here, we describe new function for yeast Sem1. We show that sem1 mutants are impaired messenger RNA (mRNA) export and transcription elongation, induce strong transcription-associated hyper-recombination phenotypes. Importantly, Sem1, independent RP, functionally linked to mRNA pathway. Biochemical analyses revealed that, addition Sem1...
RNA polymerases frequently deal with a number of obstacles during transcription elongation that need to be removed for resumption. One important type hindrance consists DNA lesions, which are by transcription-coupled repair (TC-NER), specific sub-pathway nucleotide excision repair. To improve our knowledge and its coupling TC-NER, we used the yeast library non-essential knock-out mutations screen genes conferring resistance transcription-elongation inhibitor mycophenolic acid DNA-damaging...
Laminopathies are diseases characterized by defects in nuclear envelope structure. A well-known example is Emery-Dreifuss muscular dystrophy, which caused mutations the human lamin A/C and emerin genes. While most proteins ubiquitously expressed, laminopathies often affect only a subset of tissues. The molecular mechanisms underlying these tissue-specific manifestations remain elusive. We hypothesize that different functional subclasses genes might be differentially affected specific...
The complex relationship between ontogeny and phylogeny has been the subject of attention controversy since von Baer’s formulations in 19th century. classic concept that embryogenesis progresses from clade general features to species-specific characters often revisited. It become accepted embryos a show maximum morphological similarity at so-called phylotypic period (i.e., during mid-embryogenesis). According hourglass model, body plan conservation would depend on constrained molecular...
Nuclear pore complexes consist of several subcomplexes. The NUP107 complex is important for nucleocytoplasmic transport, nuclear envelope assembly, and kinetochore function. However, the underlying molecular mechanisms roles individual members remain elusive. We report first description a genetic disruption in metazoan. Caenorhabditis elegans NUP107/npp-5 mutants display temperature-dependent lethality. Surprisingly, NPP-5 dispensable incorporation most nucleoporins into pores protein...
SUMMARY Chromatin Immunoprecipitation (ChIP) and Co-Immunoprecipitation (CoIP) assays are common approaches to characterize the genomic localization protein interactors, respectively, for a of interest. However, these require use specific antibodies, which often face sensitivity specificity issues. Based on TurboID, we developed PLAMseq (Proximity Labelled Affinity-purified Mass spectrometry plus sequencing), enables, in same workflow, identify loci interacting proteome Moreover, can also be...
Gene expression in eukaryotes is an essential process that includes transcription, RNA processing, and export. One important player this interface the poly(A)(+)-RNA-binding protein Nab2, which regulates mRNA poly(A)(+)-tail length Here we show Nab2 has additional roles during tRNA metabolism, ribosomal subunit associated with entire open reading frame of actively transcribed polymerase (RNAP) II III genes. As a consequence, nab2 mutations confer translation defects are detected by polysome...
DNA replication produces a global disorganization of chromatin structure that takes hours to be restored. However, how these rearrangements affect the regulation gene expression and maintenance cell identity is not clear. Here, we use ChOR-seq ChrRNA-seq experiments analyze RNA polymerase II (RNAPII) activity nascent synthesis during first after in human cells. We observe transcription elongation rapidly reactivated but RNAPII abundance distribution are altered, producing heterogeneous...
The THSC/TREX-2 complex of Saccharomyces cerevisiae mediates the anchoring transcribed genes to nuclear pore, linking transcription elongation with mRNA export and genome stability, as shown for specific reporters. However, it is still unknown whether function TREX-2 global reason its relevant role in integrity. Here, by studying two representative subunits, Thp1 Sac3, we show that has a genome-wide gene expression. Both proteins similar distributions along genome, gradient disposition at...
Formation of a ribonucleoprotein particle (mRNP) competent for export requires the coupling transcription with mRNA processing and RNA export. A key link between these processes is provided by THO complex. To progress in our understanding this coupling, we have performed search suppressors defect caused hpr1Δ mutation. This has permitted us to identify mutations genes polymerase II mediator component Med10, Sch9 protein kinase, Ypr045c protein. We report role elongation (Thp3) Csn12 COP9...
Noncoding RNA has a proven ability to direct and regulate chromatin modifications by acting as scaffolds between DNA histone-modifying complexes. However, it is unknown if ncRNA plays any role in replication epigenome maintenance, including histone eviction reinstallment of after genome duplication. Isolation nascent identified large number RNA-binding proteins addition components the epigenetic maintenance machinery. Here, we isolated characterized long short RNAs associated with at active...