A5028750250- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- Protist diversity and phylogeny
- Epigenetics and DNA Methylation
- Genetic and Kidney Cyst Diseases
- Cancer-related Molecular Pathways
- Histone Deacetylase Inhibitors Research
- Chromosomal and Genetic Variations
- Peptidase Inhibition and Analysis
- Agriculture and Rural Development Research
- Plant Gene Expression Analysis
- Reproductive Biology and Fertility
- Genetic Syndromes and Imprinting
- Plant Virus Research Studies
- Signaling Pathways in Disease
- Fungal and yeast genetics research
- Memory and Neural Mechanisms
- Viral-associated cancers and disorders
- Protein Degradation and Inhibitors
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Circadian rhythm and melatonin
University of Basel
2012-2024
Institut de Biochimie et Génétique Cellulaires
2018
Google (United States)
2018
Novartis (Switzerland)
2008-2010
Friedrich Miescher Institute
2006-2010
University of Geneva
2001-2006
Centre National de la Recherche Scientifique
1998-2005
Université de Bordeaux
2005
Université de Rennes
1998-2004
Laboratoire de Biologie du Développement
2000
Posttranslational modifications play important roles in regulating protein structure and function. Histone deacetylase 6 (HDAC6) is a mostly cytoplasmic class II HDAC, which has unique with two catalytic domains domain binding ubiquitin high affinity. This enzyme was recently identified as multisubstrate that can act on acetylated histone tails, α-tubulin Hsp90. To investigate the vivo functions of HDAC6 relevance tubulin acetylation/deacetylation, we targeted gene by homologous...
Histone deacetylases (HDACs) regulate gene expression by deacetylating histones and also modulate the acetylation of a number nonhistone proteins, thus impinging on various cellular processes. Here, we analyzed major class I enzymes HDAC1 HDAC2 in primary mouse fibroblasts B-cell lineage. Fibroblasts lacking both fail to proliferate culture exhibit strong cell cycle block G1 phase that is associated with up-regulation CDK inhibitors p21(WAF1/CIP1) p57(Kip2) corresponding mRNAs. This...
We have previously reported on the cloning of XlEg5, a <i>Xenopus laevis</i> kinesin-related protein from the<i>bimC</i> family (Le Guellec, R., Paris, J., Couturier, A., Roghi, C., and Philippe, M. (1991) <i>Mol. Cell. Biol.</i> 11, 3395–3408) as well pEg2, an Aurora-related serine/threonine kinase (Roghi, Giet, Uzbekov, Morin, N., Chartrain, I., Le Dorée, M., Prigent, C. (1998) <i>J. Cell Sci.</i> 111, 557–572). Inhibition either XlEg5 or pEg2 activity during mitosis in <i>Xenopus</i> egg...
Equal mitotic chromosome segregation is critical for genome integrity and monitored by the spindle assembly checkpoint (SAC). We have previously shown that consensus phosphorylation motif of essential SAC kinase Monopolar 1 (Mps1) very similar to Polo-like (Plk1). This prompted us ask whether human Plk1 cooperates with Mps1 in signaling. Here, we demonstrate promotes signaling at kinetochores through least two substrates, including KNL-1 itself. As a result, activity enhances catalytic as...
Resource18 August 2016Open Access Transparent process Quantitative analysis of human centrosome architecture by targeted proteomics and fluorescence imaging Manuel Bauer Biozentrum, University Basel, Switzerland Search for more papers this author Fabien Cubizolles Alexander Schmidt Erich A Nigg Corresponding Author [email protected] orcid.org/0000-0003-4835-5719 Information Bauer1,2,‡, Cubizolles1,‡, Schmidt1 *,1 1Biozentrum, 2Present address: Tecan Group Ltd., Männedorf, ‡These authors...
The kinase Bub1 functions in the spindle assembly checkpoint (SAC) and chromosome congression, but role of its catalytic activity remains controversial. Here, we use two novel inhibitors, BAY-320 BAY-524, to demonstrate potent inhibition both vitro intact cells. Then, compared cellular phenotypes HeLa RPE1 cells with those protein depletion, indicative or scaffolding functions, respectively. affected association Shugoshin chromosomal passenger complex (CPC), without abolishing global Aurora...
Association of the condensin multiprotein complex with chromatin is required for chromosome condensation at mitosis. What regulates targeting to largely unknown. We previously showed that nuclear A kinase–anchoring protein, AKAP95, implicated in condensation. demonstrate here AKAP95 acts as a protein human chromosome-associated (hCAP)-D2/Eg7, component complex, chromosomes. In HeLa cell mitotic extract, redistributes from matrix chromatin. When association prevented, does not condense....
The putative chromatin remodeling enzyme Plk1-interacting checkpoint helicase (PICH) was discovered as an interaction partner and substrate of the mitotic kinase Plk1. During mitosis PICH associates with centromeres kinetochores and, most interestingly, constitutes a robust marker for ultrafine DNA bridges (UFBs) that connect separating chromatids in anaphase cells. precise roles remain to be clarified. Here, we have used antibody microinjection siRNA-rescue experiments study function...
Deregulation of centriole duplication has been implicated in cancer and primary microcephaly. Accordingly, it is important to understand how key factors are regulated. E3 ubiquitin ligases have controlling the levels several factors, including PLK4, STIL SAS-6, but precise mechanisms ensuring homeostasis remain be fully understood. Here, we combined proteomics approaches with use MLN4924, a generic inhibitor SCF ligases, monitor changes cellular abundance factors. We identified human as...
We have isolated a cDNA, Eg7, corresponding to Xenopus maternal mRNA, which is polyadenylated in mature oocytes and deadenylated early embryos. This mRNA encodes protein, pEg7, whose expression strongly increased during oocyte maturation. The tissue cell pattern of pEg7 indicates that this protein only readily detected cultured cells germ cells. Immunolocalization concentrates onto chromosomes mitosis. A similar localization observed when sperm chromatin allowed form mitotic cytostatic...
Cell cycle dynamics and localization of condensins--multiprotein complexes involved in late stages mitotic chromosome condensation--were studied Xenopus laevis XL2 cell line. Western blot analysis synchronized cells showed that the ratio levels both pEg7 XCAP-E to beta-tubulin remains almost constant from G1 M phase. were localized chromosomes detected interphase nuclei. Immunostaining for condensins nucleolar proteins UBF, fibrillarin B23 revealed are granular component nucleolus. Nucleolar...
The 13 S condensin complex plays a crucial role in the condensation and segregation of two sets chromosomes during mitosis vivo as well cell-free extracts. This complex, conserved from yeast to human, contains heterodimer structural maintenance chromosome (SMC) family proteins three additional non-SMC subunits. We have investigated expression component XCAP-D2 Xenopus laevis oocytes. When studied meiotic maturation, starts accumulate at time germinal vesicle breakdown reaches its maximal...
Abstract The maintenance of correct chromosome number (euploidy) during cell division is ensured by a highly conserved surveillance mechanism termed ‘spindle assembly checkpoint’ which safeguards segregation delaying anaphase onset until all chromosomes are properly bi-oriented on the spindle apparatus. mitotic kinase BUB1 (budding uninhibited benzimidazoles 1) was reported to contribute both congression and checkpoint function, yet role catalytic activity in these processes remains matter...