Centromeres are heterochromatic in many organisms, but the mitotic function of this silent chromatin remains unknown. During cell division, newly replicated sister chromatids must cohere until anaphase when Scc1/Rad21-mediated cohesion is destroyed. In metazoans, chromosome arm cohesins dissociate during prophase, leaving centromeres as only linkage before anaphase. It not known what distinguishes centromere from cohesion. Fission yeast Swi6 (a Heterochromatin protein 1 counterpart) a...

The ura4+ gene displays phenotypes consistent with variegated expression when inserted at 11 sites throughout fission yeast centromere 1. An abrupt transition occurs between the zone of centromeric repression and two adjacent expressed sites. Mutations in six genes alleviate silent-mating type loci from a site to silent locus, mat3-M. Defects all affect telomeres three tested. clr4-S5 rik1-304 mutations cause most dramatic derepression out within cen1. All had only slight or intermediate...

Centromeres attach chromosomes to the spindle during mitosis, thereby ensuring equal distribution of into daughter cells. Transcriptionally silent heterochromatin unknown function is associated with centromeres in many organisms. In fission yeast Schizosaccharomyces pombe , mating-type loci, centromeres, and telomeres are assembled heterochromatin-like domains. The Swi6 chromodomain protein affects this silencing, now it shown that Swi6p localizes these three chromosomal regions. cells...

ABSTRACT Transcriptional silencing is known to occur at centromeres, telomeres and the mating type region in nucleus of fission yeast, Schizosaccharomyces pombe. Mating-type factors have previously been shown also affect transcriptional repression within centromeres some extent telomeres. Mutations clr4+, rik1+ swi6+ genes dramatically reduce certain centromeric regions cause elevated chromosome loss rates. Recently, Swi6p was found co-localise with three silent chromosomal regions. Here...

The spindle checkpoint ensures proper chromosome segregation by delaying anaphase until all chromosomes are correctly attached to the mitotic spindle. We investigated role of fission yeast bub1 gene in function and unperturbed mitoses. find that bub1+ is essential for response damage defects centromere function. Activation results recruitment Bub1 centromeres a delay completion mitosis. show also has crucial normal, Loss causes lag on an increased frequency loss. Such genomic instability...

In budding yeast and humans, cohesion establishment during S phase requires the acetyltransferase Eco1/Esco1-2, which acetylates cohesin subunit Smc3 on two conserved lysine residues. Whether is sole Eco1/Esco1-2 effector how acetylation promotes are unknown. fission (Schizosaccharomyces pombe), as in binding to G(1) chromosomes dynamic unloading reaction stimulated by Wpl1 (human ortholog, Wapl). During phase, a subpopulation of becomes stably bound chromatin an Eso1 (fission...

Several lines of evidence suggest that kinetochores are organizing centers for the spindle checkpoint response and synthesis a "wait anaphase" signal in cases incomplete or improper kinetochore-microtubule attachment. Here we characterize Schizosaccharomyces pombe Bub3p study recruitment components to kinetochores. We demonstrate by chromatin immunoprecipitation they all interact with central domain centromeres, consistent their role monitoring interactions. Bub1p dependent upon one another,...

An interference assay has been devised in Schizosaccharomyces pombe to rapidly identify and clone genes involved chromosome segregation. Random S.pombe cDNAs were overexpressed from an inducible promoter a strain carrying additional, non-essential minichromosome. Overexpression of derived four genes, two known (nda3+and ubc4+, encoding beta-tubulin ubiquitin conjugating enzyme, respectively) unknown, named mlo2+ mlo3+ (missegregation & lethal when over expressed) caused phenotypes consistent...

Increasing evidence implicates cohesin in the control of gene expression. Here we report first analysis cohesin-dependent regulation fission yeast. Global expression profiling mis4-367 loader mutant identified a small number upregulated and downregulated genes within subtelomeric domains (SD). These 20- to 40-kb regions between chromosome arm euchromatin telomere-proximal heterochromatin are characterized by combination (methylated lysine 4 on histone H3/methylated Tysine 9 H3 [H3K4me])...

We have isolated a fission yeast mutant,<i>mts5-1</i>, in screen for mutations that confer both methyl 2-benzimidazolecarbamate resistance (MBC^R) and temperature sensitivity (ts) on <i>Schizosaccharomyces pombe</i>. This has previously the 26 S proteasome subunits Mts2, Mts3, Mts4. show mutation <i>mts5-1</i>strain occurs <i>pad1</i> ⁺ gene.<i>pad1</i> was originally multicopy plasmid capable of conferring staurosporine wild type strain. <i>mts5-1/pad1-1</i> similar...

Journal Article Isolation of telomeric DNA from the filamentous fungus Podospora anserina and construction a self-replicating linear plasmid showing high transformation frequency Get access Jean-Paul Javerzat, Javerzat Search for other works by this author on: Oxford Academic PubMed Google Scholar Vasker Bhattacherjee, Bhattacherjee 1MRC Human Genetics Unit, Western General HospitalCrewe Road, Edinburgh EH4 2XU, UK Christian Barreau * To whom correspondence should be addressed Nucleic Acids...

Heterochromatin assembly in fission yeast relies on the processing of cognate noncoding RNAs by both RNA interference and exosome degradation pathways. Recent evidence indicates that splicing factors facilitate cotranscriptional centromeric transcripts into small interfering (siRNAs). In contrast, how contributes to heterochromatin whether it also upon were unknown. We provide here Spf30 is a factor involved pathway silencing. Dis3, main RNase, colocalize at euchromatic genes. At...

Fission yeast centromeres are transcriptionally silent and form a heterochromatin-like structure essential for normal centromere function; this appears analogous to heterochromatin position effect variegation in other eukaryotes. Conditional mutations three genes designated cep (centromere enhancer of effect) were found enhance transcriptional silencing within centromeres. Cloning the cep1+ andcep2 + by functional complementation revealed that they identical previously described genespad1...