A5071910835- Genomics and Chromatin Dynamics
- Microtubule and mitosis dynamics
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- DNA Repair Mechanisms
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Fungal and yeast genetics research
- Plant Virus Research Studies
- Plant nutrient uptake and metabolism
- Protein Degradation and Inhibitors
- Plant and Fungal Interactions Research
- Cellular transport and secretion
- DNA and Nucleic Acid Chemistry
- Plant-Microbe Interactions and Immunity
- Polysaccharides and Plant Cell Walls
- Chromosomal and Genetic Variations
- RNA modifications and cancer
- Plant and animal studies
- Bacterial Genetics and Biotechnology
Université Claude Bernard Lyon 1
2011-2022
Centre National de la Recherche Scientifique
2011-2022
École Normale Supérieure de Lyon
2011-2022
Laboratoire de Biologie et Modélisation de la Cellule
2001-2021
Clinique Claude-Bernard
2019
Inserm
2016
Wellcome Centre for Cell Biology
2004-2014
University of Edinburgh
2003-2014
Laboratoire de Reproduction et Développement des Plantes
2002-2003
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2002
The spindle checkpoint is a surveillance system acting in mitosis to delay anaphase onset until all chromosomes are properly attached the mitotic spindle. When activated, Mad2 and Mad3 proteins directly bind inhibit Cdc20, which an essential activator of E3 ubiquitin ligase known as anaphase-promoting complex (APC). satisfied, Cdc20-APC activated polyubiquitinates securin cyclin, leading dissolution sister chromatid cohesion progression. Several protein kinases play critical roles signaling,...
Recognition of self-pollen during the self-incompatibility response in Brassica oleracea is mediated by binding a secreted peptide (the S locus cysteine-rich protein) to receptor kinase (SRK), member plant (PRK) superfamily. Here, we describe characterization three proteins that interact with cytosolic domain SRK. A B. homolog Arabidopsis kinase-associated protein phosphatase was shown and dephosphorylate SRK itself phosphorylated Yeast (Saccharomyces cerevisiae) two-hybrid screens...
Fission yeast has two members of the Shugoshin family, Sgo1 and Sgo2. Although clearly been established as a protector centromere cohesion in meiosis I, roles Sgo2 remain elusive. Here we show that is required to ensure proper chromosome biorientation upon recovery from prolonged spindle checkpoint arrest. Consistent with this, essential for maintaining Passenger proteins on centromeres activation. Interestingly, lack more penetrant effect localization Survivin than other INCENP Aurora B,...
Functional links connecting gene transcription and condensin-mediated chromosome condensation have been established in species ranging from prokaryotes to vertebrates. However, the exact nature of these remains misunderstood. Here we show fission yeast that 3' end RNA processing factor Swd2.2, a component Cleavage Polyadenylation Factor (CPF), is negative regulator condensation. Lack Swd2.2 does not affect assembly CPF but reduces its association with chromatin. This causes only limited,...
The over-expression of RNase H1 enables the progression replication forks under stress and safeguards genome stability. most common interpretation these observations is that removes R-loops, whose formation would hinder fork progression. Here we challenge this model using an innovative strategy to rapidly deliver ready-made Escherichia coli (RnhA) in live cells. We show RnhA enriched vicinity active it rescues different conditions. However, had no impact on R-loops mapped by DRIP or...
Several lines of evidence suggest that kinetochores are organizing centers for the spindle checkpoint response and synthesis a "wait anaphase" signal in cases incomplete or improper kinetochore-microtubule attachment. Here we characterize Schizosaccharomyces pombe Bub3p study recruitment components to kinetochores. We demonstrate by chromatin immunoprecipitation they all interact with central domain centromeres, consistent their role monitoring interactions. Bub1p dependent upon one another,...
Abstract Three-stranded R-loop structures have been associated with genomic instability phenotypes. What underlies their wide-ranging effects on genome stability remains poorly understood. Here we combined biochemical and atomic force microscopy approaches single molecule footprinting to demonstrate that R-loops formed at the model Airn locus in vitro adopt a defined set of three-dimensional conformations characterized by distinct shapes volumes, which call objects. Interestingly, show these...
The mechanisms leading to the accumulation of SMC complexes condensins around specific transcription units remain unclear. Observations made in bacteria suggested that RNA polymerases (RNAPs) constitute an obstacle translocation, particularly when RNAP and travel opposite directions. Here we show fission yeast gene termini harbour intrinsic condensin-accumulating features whatever orientation transcription, which attribute frequent backtracking at ends. Consistent with this, relocate...
Although critical for spindle checkpoint signaling, the role kinetochores play in anaphase promoting complex (APC) inhibition remains unclear. Here we show that proteins are severely depleted from unattached fission yeast cells lacking Bub3p. Surprisingly, a robust mitotic arrest is maintained majority of bub3 Delta cells, yet they die, suggesting Bub3p essential successful recovery. During recovery, two defects observed: (1) mis-segregate chromosomes and (2) onset significantly delayed. We...
Condensin-mediated chromosome condensation is essential for genome stability upon cell division. Genetic studies have indicated that the association of condensin with chromatin intimately linked to gene transcription, but what transcription-associated feature(s) direct(s) accumulation remains unclear. Here we show in fission yeast becomes strikingly enriched at RNA Pol III-transcribed genes when Swd2.2 and Sen1, two factors involved transcription process, are simultaneously deleted. Sen1 an...
Condensin is a central regulator of mitotic genome structure with mutants showing poorly condensed chromosomes and profound segregation defects. Here, we identify NCT, complex comprising the Nrc1 BET-family tandem bromodomain protein (SPAC631.02), casein kinase II (CKII), several TAFs, as condensin function. We show that NCT bind similar genomic regions but only briefly colocalize during periods chromosome condensation decondensation. This pattern binding at core centromere, region maximal...
During mitosis, the spindle assembly checkpoint (SAC) responds to faulty attachments between kinetochores and mitotic by imposing a metaphase arrest until defect is corrected, thereby preventing chromosome missegregation. A genetic screen isolate SAC mutants in fission yeast yielded point mutations three genes: mad1, bub3, bub1. The bub1-A78V mutant of particular interest because it produces wild-type amount protein that mutated conserved but uncharacterized Mad3-like region Bub1p....